Job ID = 14520952 SRX = SRX9431258 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6124334 spots for SRR12979552/SRR12979552.sra Written 6124334 spots for SRR12979552/SRR12979552.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:38 6124334 reads; of these: 6124334 (100.00%) were unpaired; of these: 580529 (9.48%) aligned 0 times 4788668 (78.19%) aligned exactly 1 time 755137 (12.33%) aligned >1 times 90.52% overall alignment rate Time searching: 00:03:38 Overall time: 00:03:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 966392 / 5543805 = 0.1743 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:15: 1000000 INFO @ Sat, 15 Jan 2022 20:16:22: 2000000 INFO @ Sat, 15 Jan 2022 20:16:28: 3000000 INFO @ Sat, 15 Jan 2022 20:16:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:38: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:16:38: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:16:38: #1 total tags in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:16:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:16:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:16:38: #1 tags after filtering in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:16:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:16:38: #1 finished! INFO @ Sat, 15 Jan 2022 20:16:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:16:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:16:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:38: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:16:38: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:16:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:16:44: 1000000 INFO @ Sat, 15 Jan 2022 20:16:50: 2000000 INFO @ Sat, 15 Jan 2022 20:16:56: 3000000 INFO @ Sat, 15 Jan 2022 20:17:02: 4000000 INFO @ Sat, 15 Jan 2022 20:17:06: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:17:06: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:17:06: #1 total tags in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:17:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:06: #1 tags after filtering in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:17:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:06: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:06: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:17:06: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:16: 1000000 INFO @ Sat, 15 Jan 2022 20:17:22: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:17:29: 3000000 INFO @ Sat, 15 Jan 2022 20:17:36: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:17:40: #1 tag size is determined as 82 bps INFO @ Sat, 15 Jan 2022 20:17:40: #1 tag size = 82 INFO @ Sat, 15 Jan 2022 20:17:40: #1 total tags in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:17:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:17:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:17:40: #1 tags after filtering in treatment: 4577413 INFO @ Sat, 15 Jan 2022 20:17:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:17:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:17:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:17:40: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 20:17:40: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:17:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431258/SRX9431258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling