Job ID = 14520909 SRX = SRX9431246 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6030766 spots for SRR12979540/SRR12979540.sra Written 6030766 spots for SRR12979540/SRR12979540.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:11 6030766 reads; of these: 6030766 (100.00%) were unpaired; of these: 652368 (10.82%) aligned 0 times 4634004 (76.84%) aligned exactly 1 time 744394 (12.34%) aligned >1 times 89.18% overall alignment rate Time searching: 00:01:11 Overall time: 00:01:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1012470 / 5378398 = 0.1882 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:23: 1000000 INFO @ Sat, 15 Jan 2022 20:09:30: 2000000 INFO @ Sat, 15 Jan 2022 20:09:36: 3000000 INFO @ Sat, 15 Jan 2022 20:09:42: 4000000 INFO @ Sat, 15 Jan 2022 20:09:44: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:09:44: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:09:44: #1 total tags in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:09:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:44: #1 tags after filtering in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:09:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:09:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:44: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:09:44: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:53: 1000000 INFO @ Sat, 15 Jan 2022 20:09:58: 2000000 INFO @ Sat, 15 Jan 2022 20:10:04: 3000000 INFO @ Sat, 15 Jan 2022 20:10:09: 4000000 INFO @ Sat, 15 Jan 2022 20:10:11: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:10:11: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:10:11: #1 total tags in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:10:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:11: #1 tags after filtering in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:10:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:10:11: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:11: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:10:11: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:10:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:10:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:10:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:10:23: 1000000 INFO @ Sat, 15 Jan 2022 20:10:28: 2000000 INFO @ Sat, 15 Jan 2022 20:10:33: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:10:38: 4000000 INFO @ Sat, 15 Jan 2022 20:10:40: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:10:40: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:10:40: #1 total tags in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:10:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:10:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:10:40: #1 tags after filtering in treatment: 4365928 INFO @ Sat, 15 Jan 2022 20:10:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:10:40: #1 finished! INFO @ Sat, 15 Jan 2022 20:10:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:10:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:10:40: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 20:10:40: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:10:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431246/SRX9431246.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。