Job ID = 14520907 SRX = SRX9431244 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 4664305 spots for SRR12979538/SRR12979538.sra Written 4664305 spots for SRR12979538/SRR12979538.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:52 4664305 reads; of these: 4664305 (100.00%) were unpaired; of these: 490278 (10.51%) aligned 0 times 3578629 (76.72%) aligned exactly 1 time 595398 (12.76%) aligned >1 times 89.49% overall alignment rate Time searching: 00:00:52 Overall time: 00:00:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 711336 / 4174027 = 0.1704 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:08:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:08:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:08:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:08:43: 1000000 INFO @ Sat, 15 Jan 2022 20:08:50: 2000000 INFO @ Sat, 15 Jan 2022 20:08:57: 3000000 INFO @ Sat, 15 Jan 2022 20:09:00: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:09:00: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:09:00: #1 total tags in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:00: #1 tags after filtering in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:09:00: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:00: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:09:00: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:12: 1000000 INFO @ Sat, 15 Jan 2022 20:09:20: 2000000 INFO @ Sat, 15 Jan 2022 20:09:27: 3000000 INFO @ Sat, 15 Jan 2022 20:09:30: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:09:30: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:09:30: #1 total tags in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:30: #1 tags after filtering in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:09:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:30: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:09:30: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:09:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:09:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:09:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:09:41: 1000000 INFO @ Sat, 15 Jan 2022 20:09:47: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:09:53: 3000000 INFO @ Sat, 15 Jan 2022 20:09:55: #1 tag size is determined as 68 bps INFO @ Sat, 15 Jan 2022 20:09:55: #1 tag size = 68 INFO @ Sat, 15 Jan 2022 20:09:55: #1 total tags in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:09:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:09:55: #1 tags after filtering in treatment: 3462691 INFO @ Sat, 15 Jan 2022 20:09:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:09:55: #1 finished! INFO @ Sat, 15 Jan 2022 20:09:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:09:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:09:55: #2 number of paired peaks: 32 WARNING @ Sat, 15 Jan 2022 20:09:55: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:09:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9431244/SRX9431244.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。