Job ID = 14521801 SRX = SRX9399181 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9111679 spots for SRR12935462/SRR12935462.sra Written 9111679 spots for SRR12935462/SRR12935462.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:49 9111679 reads; of these: 9111679 (100.00%) were paired; of these: 1022773 (11.22%) aligned concordantly 0 times 6597088 (72.40%) aligned concordantly exactly 1 time 1491818 (16.37%) aligned concordantly >1 times ---- 1022773 pairs aligned concordantly 0 times; of these: 83787 (8.19%) aligned discordantly 1 time ---- 938986 pairs aligned 0 times concordantly or discordantly; of these: 1877972 mates make up the pairs; of these: 1626740 (86.62%) aligned 0 times 178157 (9.49%) aligned exactly 1 time 73075 (3.89%) aligned >1 times 91.07% overall alignment rate Time searching: 00:06:49 Overall time: 00:06:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2176504 / 8158355 = 0.2668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:48:32: 1000000 INFO @ Sat, 15 Jan 2022 21:48:38: 2000000 INFO @ Sat, 15 Jan 2022 21:48:44: 3000000 INFO @ Sat, 15 Jan 2022 21:48:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:56: 5000000 INFO @ Sat, 15 Jan 2022 21:48:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:02: 6000000 INFO @ Sat, 15 Jan 2022 21:49:02: 1000000 INFO @ Sat, 15 Jan 2022 21:49:08: 7000000 INFO @ Sat, 15 Jan 2022 21:49:08: 2000000 INFO @ Sat, 15 Jan 2022 21:49:14: 8000000 INFO @ Sat, 15 Jan 2022 21:49:14: 3000000 INFO @ Sat, 15 Jan 2022 21:49:20: 9000000 INFO @ Sat, 15 Jan 2022 21:49:20: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:26: 10000000 INFO @ Sat, 15 Jan 2022 21:49:26: 5000000 INFO @ Sat, 15 Jan 2022 21:49:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:32: 11000000 INFO @ Sat, 15 Jan 2022 21:49:32: 6000000 INFO @ Sat, 15 Jan 2022 21:49:32: 1000000 INFO @ Sat, 15 Jan 2022 21:49:38: 12000000 INFO @ Sat, 15 Jan 2022 21:49:38: 7000000 INFO @ Sat, 15 Jan 2022 21:49:39: 2000000 INFO @ Sat, 15 Jan 2022 21:49:39: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:49:39: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:49:39: #1 total tags in treatment: 5924095 INFO @ Sat, 15 Jan 2022 21:49:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:49:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:49:39: #1 tags after filtering in treatment: 3699044 INFO @ Sat, 15 Jan 2022 21:49:39: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 21:49:39: #1 finished! INFO @ Sat, 15 Jan 2022 21:49:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:49:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:49:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:49:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:49:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:49:45: 8000000 INFO @ Sat, 15 Jan 2022 21:49:45: 3000000 INFO @ Sat, 15 Jan 2022 21:49:50: 9000000 INFO @ Sat, 15 Jan 2022 21:49:51: 4000000 INFO @ Sat, 15 Jan 2022 21:49:56: 10000000 INFO @ Sat, 15 Jan 2022 21:49:57: 5000000 INFO @ Sat, 15 Jan 2022 21:50:02: 11000000 INFO @ Sat, 15 Jan 2022 21:50:03: 6000000 INFO @ Sat, 15 Jan 2022 21:50:08: 12000000 INFO @ Sat, 15 Jan 2022 21:50:09: 7000000 INFO @ Sat, 15 Jan 2022 21:50:10: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:50:10: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:50:10: #1 total tags in treatment: 5924095 INFO @ Sat, 15 Jan 2022 21:50:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:50:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:50:10: #1 tags after filtering in treatment: 3699044 INFO @ Sat, 15 Jan 2022 21:50:10: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 21:50:10: #1 finished! INFO @ Sat, 15 Jan 2022 21:50:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:50:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:50:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:50:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:50:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:50:15: 8000000 INFO @ Sat, 15 Jan 2022 21:50:21: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:50:26: 10000000 INFO @ Sat, 15 Jan 2022 21:50:32: 11000000 INFO @ Sat, 15 Jan 2022 21:50:38: 12000000 INFO @ Sat, 15 Jan 2022 21:50:40: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:50:40: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:50:40: #1 total tags in treatment: 5924095 INFO @ Sat, 15 Jan 2022 21:50:40: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:50:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:50:40: #1 tags after filtering in treatment: 3699044 INFO @ Sat, 15 Jan 2022 21:50:40: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 21:50:40: #1 finished! INFO @ Sat, 15 Jan 2022 21:50:40: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:50:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:50:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:50:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:50:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399181/SRX9399181.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling