Job ID = 14521766 SRX = SRX9399176 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10834619 spots for SRR12935457/SRR12935457.sra Written 10834619 spots for SRR12935457/SRR12935457.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:36 10834619 reads; of these: 10834619 (100.00%) were paired; of these: 1753663 (16.19%) aligned concordantly 0 times 7897113 (72.89%) aligned concordantly exactly 1 time 1183843 (10.93%) aligned concordantly >1 times ---- 1753663 pairs aligned concordantly 0 times; of these: 434888 (24.80%) aligned discordantly 1 time ---- 1318775 pairs aligned 0 times concordantly or discordantly; of these: 2637550 mates make up the pairs; of these: 2163802 (82.04%) aligned 0 times 314118 (11.91%) aligned exactly 1 time 159630 (6.05%) aligned >1 times 90.01% overall alignment rate Time searching: 00:08:36 Overall time: 00:08:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1539729 / 9310544 = 0.1654 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:48:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:48:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:48:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:07: 1000000 INFO @ Sat, 15 Jan 2022 21:49:16: 2000000 INFO @ Sat, 15 Jan 2022 21:49:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:28: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:28: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:49:33: 4000000 INFO @ Sat, 15 Jan 2022 21:49:35: 1000000 INFO @ Sat, 15 Jan 2022 21:49:41: 2000000 INFO @ Sat, 15 Jan 2022 21:49:42: 5000000 INFO @ Sat, 15 Jan 2022 21:49:48: 3000000 INFO @ Sat, 15 Jan 2022 21:49:51: 6000000 INFO @ Sat, 15 Jan 2022 21:49:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:49:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:49:58: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:49:58: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:50:00: 7000000 INFO @ Sat, 15 Jan 2022 21:50:01: 5000000 INFO @ Sat, 15 Jan 2022 21:50:07: 6000000 INFO @ Sat, 15 Jan 2022 21:50:09: 1000000 INFO @ Sat, 15 Jan 2022 21:50:10: 8000000 INFO @ Sat, 15 Jan 2022 21:50:14: 7000000 INFO @ Sat, 15 Jan 2022 21:50:18: 2000000 INFO @ Sat, 15 Jan 2022 21:50:19: 9000000 INFO @ Sat, 15 Jan 2022 21:50:21: 8000000 INFO @ Sat, 15 Jan 2022 21:50:28: 9000000 INFO @ Sat, 15 Jan 2022 21:50:28: 3000000 INFO @ Sat, 15 Jan 2022 21:50:30: 10000000 INFO @ Sat, 15 Jan 2022 21:50:35: 10000000 INFO @ Sat, 15 Jan 2022 21:50:38: 4000000 INFO @ Sat, 15 Jan 2022 21:50:40: 11000000 INFO @ Sat, 15 Jan 2022 21:50:42: 11000000 INFO @ Sat, 15 Jan 2022 21:50:48: 5000000 INFO @ Sat, 15 Jan 2022 21:50:49: 12000000 INFO @ Sat, 15 Jan 2022 21:50:50: 12000000 INFO @ Sat, 15 Jan 2022 21:50:57: 13000000 INFO @ Sat, 15 Jan 2022 21:50:58: 6000000 INFO @ Sat, 15 Jan 2022 21:51:00: 13000000 INFO @ Sat, 15 Jan 2022 21:51:03: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:51:09: 7000000 INFO @ Sat, 15 Jan 2022 21:51:09: 14000000 INFO @ Sat, 15 Jan 2022 21:51:11: 15000000 INFO @ Sat, 15 Jan 2022 21:51:19: 16000000 INFO @ Sat, 15 Jan 2022 21:51:19: 8000000 INFO @ Sat, 15 Jan 2022 21:51:20: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:51:22: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:51:22: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:51:22: #1 total tags in treatment: 7564681 INFO @ Sat, 15 Jan 2022 21:51:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:51:22: #1 tags after filtering in treatment: 4323648 INFO @ Sat, 15 Jan 2022 21:51:22: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 21:51:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:51:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:51:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:51:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:51:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:51:29: 9000000 INFO @ Sat, 15 Jan 2022 21:51:29: 16000000 INFO @ Sat, 15 Jan 2022 21:51:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:51:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:51:33: #1 total tags in treatment: 7564681 INFO @ Sat, 15 Jan 2022 21:51:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:51:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:51:34: #1 tags after filtering in treatment: 4323648 INFO @ Sat, 15 Jan 2022 21:51:34: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 21:51:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:51:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:51:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:51:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:51:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:51:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:51:38: 10000000 INFO @ Sat, 15 Jan 2022 21:51:47: 11000000 INFO @ Sat, 15 Jan 2022 21:51:55: 12000000 INFO @ Sat, 15 Jan 2022 21:52:04: 13000000 INFO @ Sat, 15 Jan 2022 21:52:12: 14000000 INFO @ Sat, 15 Jan 2022 21:52:21: 15000000 INFO @ Sat, 15 Jan 2022 21:52:30: 16000000 INFO @ Sat, 15 Jan 2022 21:52:33: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:52:33: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:52:33: #1 total tags in treatment: 7564681 INFO @ Sat, 15 Jan 2022 21:52:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:52:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:52:33: #1 tags after filtering in treatment: 4323648 INFO @ Sat, 15 Jan 2022 21:52:33: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 21:52:33: #1 finished! INFO @ Sat, 15 Jan 2022 21:52:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:52:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:52:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:52:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:52:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399176/SRX9399176.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling