Job ID = 14521738 SRX = SRX9399168 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8560953 spots for SRR12935449/SRR12935449.sra Written 8560953 spots for SRR12935449/SRR12935449.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:01 8560953 reads; of these: 8560953 (100.00%) were paired; of these: 1849695 (21.61%) aligned concordantly 0 times 5943826 (69.43%) aligned concordantly exactly 1 time 767432 (8.96%) aligned concordantly >1 times ---- 1849695 pairs aligned concordantly 0 times; of these: 365672 (19.77%) aligned discordantly 1 time ---- 1484023 pairs aligned 0 times concordantly or discordantly; of these: 2968046 mates make up the pairs; of these: 2416557 (81.42%) aligned 0 times 405312 (13.66%) aligned exactly 1 time 146177 (4.93%) aligned >1 times 85.89% overall alignment rate Time searching: 00:06:01 Overall time: 00:06:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 903306 / 6992418 = 0.1292 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:41:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:41:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:41:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:41:50: 1000000 INFO @ Sat, 15 Jan 2022 21:41:56: 2000000 INFO @ Sat, 15 Jan 2022 21:42:03: 3000000 INFO @ Sat, 15 Jan 2022 21:42:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:17: 5000000 INFO @ Sat, 15 Jan 2022 21:42:19: 1000000 INFO @ Sat, 15 Jan 2022 21:42:24: 6000000 INFO @ Sat, 15 Jan 2022 21:42:25: 2000000 INFO @ Sat, 15 Jan 2022 21:42:30: 3000000 INFO @ Sat, 15 Jan 2022 21:42:31: 7000000 INFO @ Sat, 15 Jan 2022 21:42:36: 4000000 INFO @ Sat, 15 Jan 2022 21:42:38: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:42:42: 5000000 INFO @ Sat, 15 Jan 2022 21:42:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:42:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:42:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:42:45: 9000000 INFO @ Sat, 15 Jan 2022 21:42:48: 6000000 INFO @ Sat, 15 Jan 2022 21:42:51: 1000000 INFO @ Sat, 15 Jan 2022 21:42:53: 10000000 INFO @ Sat, 15 Jan 2022 21:42:55: 7000000 INFO @ Sat, 15 Jan 2022 21:42:58: 2000000 INFO @ Sat, 15 Jan 2022 21:43:00: 11000000 INFO @ Sat, 15 Jan 2022 21:43:02: 8000000 INFO @ Sat, 15 Jan 2022 21:43:05: 3000000 INFO @ Sat, 15 Jan 2022 21:43:08: 12000000 INFO @ Sat, 15 Jan 2022 21:43:10: 9000000 INFO @ Sat, 15 Jan 2022 21:43:11: 4000000 INFO @ Sat, 15 Jan 2022 21:43:14: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:43:14: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:43:14: #1 total tags in treatment: 5842780 INFO @ Sat, 15 Jan 2022 21:43:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:14: #1 tags after filtering in treatment: 3768929 INFO @ Sat, 15 Jan 2022 21:43:14: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:43:14: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:43:16: 10000000 INFO @ Sat, 15 Jan 2022 21:43:18: 5000000 INFO @ Sat, 15 Jan 2022 21:43:23: 11000000 INFO @ Sat, 15 Jan 2022 21:43:25: 6000000 INFO @ Sat, 15 Jan 2022 21:43:30: 12000000 INFO @ Sat, 15 Jan 2022 21:43:31: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:43:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:43:35: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:43:35: #1 total tags in treatment: 5842780 INFO @ Sat, 15 Jan 2022 21:43:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:43:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:43:36: #1 tags after filtering in treatment: 3768929 INFO @ Sat, 15 Jan 2022 21:43:36: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:43:36: #1 finished! INFO @ Sat, 15 Jan 2022 21:43:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:43:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:43:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:43:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:43:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:43:38: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:43:46: 9000000 INFO @ Sat, 15 Jan 2022 21:43:52: 10000000 INFO @ Sat, 15 Jan 2022 21:43:57: 11000000 INFO @ Sat, 15 Jan 2022 21:44:02: 12000000 INFO @ Sat, 15 Jan 2022 21:44:07: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 21:44:07: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 21:44:07: #1 total tags in treatment: 5842780 INFO @ Sat, 15 Jan 2022 21:44:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:44:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:44:07: #1 tags after filtering in treatment: 3768929 INFO @ Sat, 15 Jan 2022 21:44:07: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:44:07: #1 finished! INFO @ Sat, 15 Jan 2022 21:44:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:44:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:44:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:44:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:44:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399168/SRX9399168.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling