Job ID = 14521701 SRX = SRX9399151 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8462631 spots for SRR12935432/SRR12935432.sra Written 8462631 spots for SRR12935432/SRR12935432.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:39 8462631 reads; of these: 8462631 (100.00%) were paired; of these: 1116205 (13.19%) aligned concordantly 0 times 6663567 (78.74%) aligned concordantly exactly 1 time 682859 (8.07%) aligned concordantly >1 times ---- 1116205 pairs aligned concordantly 0 times; of these: 127046 (11.38%) aligned discordantly 1 time ---- 989159 pairs aligned 0 times concordantly or discordantly; of these: 1978318 mates make up the pairs; of these: 1642819 (83.04%) aligned 0 times 267677 (13.53%) aligned exactly 1 time 67822 (3.43%) aligned >1 times 90.29% overall alignment rate Time searching: 00:03:39 Overall time: 00:03:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1017748 / 7461131 = 0.1364 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:33:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:33:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:33:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:33:29: 1000000 INFO @ Sat, 15 Jan 2022 21:33:35: 2000000 INFO @ Sat, 15 Jan 2022 21:33:41: 3000000 INFO @ Sat, 15 Jan 2022 21:33:46: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:33:52: 5000000 INFO @ Sat, 15 Jan 2022 21:33:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:33:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:33:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:33:58: 6000000 INFO @ Sat, 15 Jan 2022 21:34:00: 1000000 INFO @ Sat, 15 Jan 2022 21:34:04: 7000000 INFO @ Sat, 15 Jan 2022 21:34:06: 2000000 INFO @ Sat, 15 Jan 2022 21:34:10: 8000000 INFO @ Sat, 15 Jan 2022 21:34:12: 3000000 INFO @ Sat, 15 Jan 2022 21:34:17: 9000000 INFO @ Sat, 15 Jan 2022 21:34:18: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:34:23: 10000000 INFO @ Sat, 15 Jan 2022 21:34:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:34:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:34:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:34:24: 5000000 INFO @ Sat, 15 Jan 2022 21:34:29: 11000000 INFO @ Sat, 15 Jan 2022 21:34:30: 1000000 INFO @ Sat, 15 Jan 2022 21:34:30: 6000000 INFO @ Sat, 15 Jan 2022 21:34:35: 12000000 INFO @ Sat, 15 Jan 2022 21:34:36: 2000000 INFO @ Sat, 15 Jan 2022 21:34:37: 7000000 INFO @ Sat, 15 Jan 2022 21:34:41: 13000000 INFO @ Sat, 15 Jan 2022 21:34:42: 3000000 INFO @ Sat, 15 Jan 2022 21:34:43: 8000000 INFO @ Sat, 15 Jan 2022 21:34:43: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:34:43: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:34:43: #1 total tags in treatment: 6335520 INFO @ Sat, 15 Jan 2022 21:34:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:34:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:34:43: #1 tags after filtering in treatment: 4048561 INFO @ Sat, 15 Jan 2022 21:34:43: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:34:43: #1 finished! INFO @ Sat, 15 Jan 2022 21:34:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:34:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:34:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:34:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:34:48: 4000000 INFO @ Sat, 15 Jan 2022 21:34:49: 9000000 INFO @ Sat, 15 Jan 2022 21:34:54: 5000000 INFO @ Sat, 15 Jan 2022 21:34:55: 10000000 INFO @ Sat, 15 Jan 2022 21:35:00: 6000000 INFO @ Sat, 15 Jan 2022 21:35:01: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:35:06: 7000000 INFO @ Sat, 15 Jan 2022 21:35:07: 12000000 INFO @ Sat, 15 Jan 2022 21:35:12: 8000000 INFO @ Sat, 15 Jan 2022 21:35:13: 13000000 INFO @ Sat, 15 Jan 2022 21:35:15: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:35:15: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:35:15: #1 total tags in treatment: 6335520 INFO @ Sat, 15 Jan 2022 21:35:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:15: #1 tags after filtering in treatment: 4048561 INFO @ Sat, 15 Jan 2022 21:35:15: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:35:15: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:35:18: 9000000 INFO @ Sat, 15 Jan 2022 21:35:26: 10000000 INFO @ Sat, 15 Jan 2022 21:35:32: 11000000 INFO @ Sat, 15 Jan 2022 21:35:37: 12000000 INFO @ Sat, 15 Jan 2022 21:35:43: 13000000 INFO @ Sat, 15 Jan 2022 21:35:44: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:35:44: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:35:44: #1 total tags in treatment: 6335520 INFO @ Sat, 15 Jan 2022 21:35:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:35:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:35:44: #1 tags after filtering in treatment: 4048561 INFO @ Sat, 15 Jan 2022 21:35:44: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 21:35:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:35:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:35:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:35:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:35:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:35:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399151/SRX9399151.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling