Job ID = 14521677 SRX = SRX9399147 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7557842 spots for SRR12935428/SRR12935428.sra Written 7557842 spots for SRR12935428/SRR12935428.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 7557842 reads; of these: 7557842 (100.00%) were paired; of these: 774941 (10.25%) aligned concordantly 0 times 6129758 (81.10%) aligned concordantly exactly 1 time 653143 (8.64%) aligned concordantly >1 times ---- 774941 pairs aligned concordantly 0 times; of these: 119470 (15.42%) aligned discordantly 1 time ---- 655471 pairs aligned 0 times concordantly or discordantly; of these: 1310942 mates make up the pairs; of these: 1054093 (80.41%) aligned 0 times 200863 (15.32%) aligned exactly 1 time 55986 (4.27%) aligned >1 times 93.03% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1094275 / 6890269 = 0.1588 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:24: 1000000 INFO @ Sat, 15 Jan 2022 21:29:28: 2000000 INFO @ Sat, 15 Jan 2022 21:29:32: 3000000 INFO @ Sat, 15 Jan 2022 21:29:36: 4000000 INFO @ Sat, 15 Jan 2022 21:29:40: 5000000 INFO @ Sat, 15 Jan 2022 21:29:44: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:29:48: 7000000 INFO @ Sat, 15 Jan 2022 21:29:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:29:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:29:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:29:53: 8000000 INFO @ Sat, 15 Jan 2022 21:29:54: 1000000 INFO @ Sat, 15 Jan 2022 21:29:57: 9000000 INFO @ Sat, 15 Jan 2022 21:29:59: 2000000 INFO @ Sat, 15 Jan 2022 21:30:01: 10000000 INFO @ Sat, 15 Jan 2022 21:30:03: 3000000 INFO @ Sat, 15 Jan 2022 21:30:06: 11000000 INFO @ Sat, 15 Jan 2022 21:30:07: 4000000 INFO @ Sat, 15 Jan 2022 21:30:09: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:30:09: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:30:09: #1 total tags in treatment: 5696019 INFO @ Sat, 15 Jan 2022 21:30:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:09: #1 tags after filtering in treatment: 3717401 INFO @ Sat, 15 Jan 2022 21:30:09: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:30:09: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:10: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:30:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:30:12: 5000000 INFO @ Sat, 15 Jan 2022 21:30:16: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:30:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:30:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:30:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:30:20: 7000000 INFO @ Sat, 15 Jan 2022 21:30:24: 1000000 INFO @ Sat, 15 Jan 2022 21:30:24: 8000000 INFO @ Sat, 15 Jan 2022 21:30:29: 9000000 INFO @ Sat, 15 Jan 2022 21:30:29: 2000000 INFO @ Sat, 15 Jan 2022 21:30:33: 10000000 INFO @ Sat, 15 Jan 2022 21:30:33: 3000000 INFO @ Sat, 15 Jan 2022 21:30:37: 11000000 INFO @ Sat, 15 Jan 2022 21:30:37: 4000000 INFO @ Sat, 15 Jan 2022 21:30:41: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:30:41: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:30:41: #1 total tags in treatment: 5696019 INFO @ Sat, 15 Jan 2022 21:30:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:30:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:30:41: #1 tags after filtering in treatment: 3717401 INFO @ Sat, 15 Jan 2022 21:30:41: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:30:41: #1 finished! INFO @ Sat, 15 Jan 2022 21:30:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:30:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:30:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:30:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:30:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:30:42: 5000000 INFO @ Sat, 15 Jan 2022 21:30:46: 6000000 INFO @ Sat, 15 Jan 2022 21:30:50: 7000000 INFO @ Sat, 15 Jan 2022 21:30:55: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:30:59: 9000000 INFO @ Sat, 15 Jan 2022 21:31:03: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:31:07: 11000000 INFO @ Sat, 15 Jan 2022 21:31:11: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:31:11: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:31:11: #1 total tags in treatment: 5696019 INFO @ Sat, 15 Jan 2022 21:31:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:31:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:31:11: #1 tags after filtering in treatment: 3717401 INFO @ Sat, 15 Jan 2022 21:31:11: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 15 Jan 2022 21:31:11: #1 finished! INFO @ Sat, 15 Jan 2022 21:31:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:31:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:31:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:31:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:31:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399147/SRX9399147.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling