Job ID = 14521590 SRX = SRX9399102 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4823355 spots for SRR12935383/SRR12935383.sra Written 4823355 spots for SRR12935383/SRR12935383.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:59 4823355 reads; of these: 4823355 (100.00%) were paired; of these: 263270 (5.46%) aligned concordantly 0 times 3534120 (73.27%) aligned concordantly exactly 1 time 1025965 (21.27%) aligned concordantly >1 times ---- 263270 pairs aligned concordantly 0 times; of these: 25354 (9.63%) aligned discordantly 1 time ---- 237916 pairs aligned 0 times concordantly or discordantly; of these: 475832 mates make up the pairs; of these: 392148 (82.41%) aligned 0 times 55945 (11.76%) aligned exactly 1 time 27739 (5.83%) aligned >1 times 95.93% overall alignment rate Time searching: 00:02:59 Overall time: 00:02:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 906747 / 4580386 = 0.1980 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:15:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:15:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:15:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:15:53: 1000000 INFO @ Sat, 15 Jan 2022 21:15:59: 2000000 INFO @ Sat, 15 Jan 2022 21:16:04: 3000000 INFO @ Sat, 15 Jan 2022 21:16:09: 4000000 INFO @ Sat, 15 Jan 2022 21:16:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:16:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:16:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:16:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:16:20: 6000000 INFO @ Sat, 15 Jan 2022 21:16:24: 1000000 INFO @ Sat, 15 Jan 2022 21:16:26: 7000000 INFO @ Sat, 15 Jan 2022 21:16:28: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:16:28: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:16:28: #1 total tags in treatment: 3654271 INFO @ Sat, 15 Jan 2022 21:16:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:29: #1 tags after filtering in treatment: 2554624 INFO @ Sat, 15 Jan 2022 21:16:29: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:16:29: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:29: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 21:16:29: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:29: 2000000 INFO @ Sat, 15 Jan 2022 21:16:34: 3000000 INFO @ Sat, 15 Jan 2022 21:16:39: 4000000 INFO @ Sat, 15 Jan 2022 21:16:45: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:16:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:16:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:16:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:16:50: 6000000 INFO @ Sat, 15 Jan 2022 21:16:52: 1000000 INFO @ Sat, 15 Jan 2022 21:16:56: 7000000 INFO @ Sat, 15 Jan 2022 21:16:57: 2000000 INFO @ Sat, 15 Jan 2022 21:16:58: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:16:58: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:16:58: #1 total tags in treatment: 3654271 INFO @ Sat, 15 Jan 2022 21:16:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:58: #1 tags after filtering in treatment: 2554624 INFO @ Sat, 15 Jan 2022 21:16:58: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:16:58: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:58: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 21:16:58: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:17:02: 3000000 INFO @ Sat, 15 Jan 2022 21:17:06: 4000000 INFO @ Sat, 15 Jan 2022 21:17:11: 5000000 INFO @ Sat, 15 Jan 2022 21:17:16: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:17:20: 7000000 INFO @ Sat, 15 Jan 2022 21:17:22: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:17:22: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:17:22: #1 total tags in treatment: 3654271 INFO @ Sat, 15 Jan 2022 21:17:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:17:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:17:22: #1 tags after filtering in treatment: 2554624 INFO @ Sat, 15 Jan 2022 21:17:22: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 21:17:22: #1 finished! INFO @ Sat, 15 Jan 2022 21:17:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:17:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:17:22: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 21:17:22: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:17:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399102/SRX9399102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。