Job ID = 14521588 SRX = SRX9399100 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4764608 spots for SRR12935381/SRR12935381.sra Written 4764608 spots for SRR12935381/SRR12935381.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 4764608 reads; of these: 4764608 (100.00%) were paired; of these: 413090 (8.67%) aligned concordantly 0 times 3494105 (73.33%) aligned concordantly exactly 1 time 857413 (18.00%) aligned concordantly >1 times ---- 413090 pairs aligned concordantly 0 times; of these: 48701 (11.79%) aligned discordantly 1 time ---- 364389 pairs aligned 0 times concordantly or discordantly; of these: 728778 mates make up the pairs; of these: 596990 (81.92%) aligned 0 times 92192 (12.65%) aligned exactly 1 time 39596 (5.43%) aligned >1 times 93.74% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 724965 / 4392696 = 0.1650 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:23: 1000000 INFO @ Sat, 15 Jan 2022 21:14:28: 2000000 INFO @ Sat, 15 Jan 2022 21:14:32: 3000000 INFO @ Sat, 15 Jan 2022 21:14:37: 4000000 INFO @ Sat, 15 Jan 2022 21:14:42: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:47: 6000000 INFO @ Sat, 15 Jan 2022 21:14:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:53: 7000000 INFO @ Sat, 15 Jan 2022 21:14:54: 1000000 INFO @ Sat, 15 Jan 2022 21:14:55: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:14:55: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:14:55: #1 total tags in treatment: 3627964 INFO @ Sat, 15 Jan 2022 21:14:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:56: #1 tags after filtering in treatment: 2580761 INFO @ Sat, 15 Jan 2022 21:14:56: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:14:56: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:56: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:14:56: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:00: 2000000 INFO @ Sat, 15 Jan 2022 21:15:06: 3000000 INFO @ Sat, 15 Jan 2022 21:15:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:15:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:15:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:15:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:15:18: 5000000 INFO @ Sat, 15 Jan 2022 21:15:24: 1000000 INFO @ Sat, 15 Jan 2022 21:15:24: 6000000 INFO @ Sat, 15 Jan 2022 21:15:29: 2000000 INFO @ Sat, 15 Jan 2022 21:15:31: 7000000 INFO @ Sat, 15 Jan 2022 21:15:34: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:15:34: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:15:34: #1 total tags in treatment: 3627964 INFO @ Sat, 15 Jan 2022 21:15:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:34: #1 tags after filtering in treatment: 2580761 INFO @ Sat, 15 Jan 2022 21:15:34: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:15:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:34: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:15:34: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:15:35: 3000000 INFO @ Sat, 15 Jan 2022 21:15:40: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:15:45: 5000000 INFO @ Sat, 15 Jan 2022 21:15:50: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:15:55: 7000000 INFO @ Sat, 15 Jan 2022 21:15:57: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 21:15:57: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 21:15:57: #1 total tags in treatment: 3627964 INFO @ Sat, 15 Jan 2022 21:15:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:57: #1 tags after filtering in treatment: 2580761 INFO @ Sat, 15 Jan 2022 21:15:57: #1 Redundant rate of treatment: 0.29 INFO @ Sat, 15 Jan 2022 21:15:57: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:57: #2 number of paired peaks: 28 WARNING @ Sat, 15 Jan 2022 21:15:57: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399100/SRX9399100.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling