Job ID = 14521752 SRX = SRX9399052 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6405840 spots for SRR12935333/SRR12935333.sra Written 6405840 spots for SRR12935333/SRR12935333.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 6405840 reads; of these: 6405840 (100.00%) were paired; of these: 1133786 (17.70%) aligned concordantly 0 times 4652940 (72.64%) aligned concordantly exactly 1 time 619114 (9.66%) aligned concordantly >1 times ---- 1133786 pairs aligned concordantly 0 times; of these: 245057 (21.61%) aligned discordantly 1 time ---- 888729 pairs aligned 0 times concordantly or discordantly; of these: 1777458 mates make up the pairs; of these: 1335850 (75.16%) aligned 0 times 339119 (19.08%) aligned exactly 1 time 102489 (5.77%) aligned >1 times 89.57% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 413662 / 5420390 = 0.0763 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:37:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:37:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:37:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:37:55: 1000000 INFO @ Sat, 15 Jan 2022 21:38:00: 2000000 INFO @ Sat, 15 Jan 2022 21:38:04: 3000000 INFO @ Sat, 15 Jan 2022 21:38:09: 4000000 INFO @ Sat, 15 Jan 2022 21:38:13: 5000000 INFO @ Sat, 15 Jan 2022 21:38:17: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:38:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:38:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:38:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:22: 7000000 INFO @ Sat, 15 Jan 2022 21:38:26: 1000000 INFO @ Sat, 15 Jan 2022 21:38:27: 8000000 INFO @ Sat, 15 Jan 2022 21:38:32: 2000000 INFO @ Sat, 15 Jan 2022 21:38:33: 9000000 INFO @ Sat, 15 Jan 2022 21:38:37: 3000000 INFO @ Sat, 15 Jan 2022 21:38:38: 10000000 INFO @ Sat, 15 Jan 2022 21:38:42: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:38:42: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:38:42: #1 total tags in treatment: 4863220 INFO @ Sat, 15 Jan 2022 21:38:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:38:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:38:42: #1 tags after filtering in treatment: 3336560 INFO @ Sat, 15 Jan 2022 21:38:42: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:38:42: #1 finished! INFO @ Sat, 15 Jan 2022 21:38:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:38:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:38:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:38:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:38:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:38:43: 4000000 INFO @ Sat, 15 Jan 2022 21:38:48: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:38:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:38:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:38:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:38:53: 6000000 INFO @ Sat, 15 Jan 2022 21:38:56: 1000000 INFO @ Sat, 15 Jan 2022 21:38:58: 7000000 INFO @ Sat, 15 Jan 2022 21:39:01: 2000000 INFO @ Sat, 15 Jan 2022 21:39:03: 8000000 INFO @ Sat, 15 Jan 2022 21:39:06: 3000000 INFO @ Sat, 15 Jan 2022 21:39:08: 9000000 INFO @ Sat, 15 Jan 2022 21:39:11: 4000000 INFO @ Sat, 15 Jan 2022 21:39:13: 10000000 INFO @ Sat, 15 Jan 2022 21:39:17: 5000000 INFO @ Sat, 15 Jan 2022 21:39:17: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:39:17: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:39:17: #1 total tags in treatment: 4863220 INFO @ Sat, 15 Jan 2022 21:39:17: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:39:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:39:17: #1 tags after filtering in treatment: 3336560 INFO @ Sat, 15 Jan 2022 21:39:17: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:39:17: #1 finished! INFO @ Sat, 15 Jan 2022 21:39:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:39:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:39:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:39:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:39:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:39:21: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:39:26: 7000000 INFO @ Sat, 15 Jan 2022 21:39:31: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:39:36: 9000000 INFO @ Sat, 15 Jan 2022 21:39:41: 10000000 INFO @ Sat, 15 Jan 2022 21:39:44: #1 tag size is determined as 40 bps INFO @ Sat, 15 Jan 2022 21:39:44: #1 tag size = 40 INFO @ Sat, 15 Jan 2022 21:39:44: #1 total tags in treatment: 4863220 INFO @ Sat, 15 Jan 2022 21:39:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:39:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:39:44: #1 tags after filtering in treatment: 3336560 INFO @ Sat, 15 Jan 2022 21:39:44: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 21:39:44: #1 finished! INFO @ Sat, 15 Jan 2022 21:39:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:39:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:39:44: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:39:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:39:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399052/SRX9399052.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling