Job ID = 14521662 SRX = SRX9399023 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3149061 spots for SRR12935305/SRR12935305.sra Written 3149061 spots for SRR12935305/SRR12935305.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:10 3149061 reads; of these: 3149061 (100.00%) were paired; of these: 612139 (19.44%) aligned concordantly 0 times 2152513 (68.35%) aligned concordantly exactly 1 time 384409 (12.21%) aligned concordantly >1 times ---- 612139 pairs aligned concordantly 0 times; of these: 254753 (41.62%) aligned discordantly 1 time ---- 357386 pairs aligned 0 times concordantly or discordantly; of these: 714772 mates make up the pairs; of these: 390662 (54.66%) aligned 0 times 202086 (28.27%) aligned exactly 1 time 122024 (17.07%) aligned >1 times 93.80% overall alignment rate Time searching: 00:02:10 Overall time: 00:02:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 230419 / 2581172 = 0.0893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:26:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:26:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:26:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:26:26: 1000000 INFO @ Sat, 15 Jan 2022 21:26:33: 2000000 INFO @ Sat, 15 Jan 2022 21:26:39: 3000000 INFO @ Sat, 15 Jan 2022 21:26:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:26:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:26:50: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:26:50: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:26:52: 5000000 INFO @ Sat, 15 Jan 2022 21:26:55: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:26:55: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:26:55: #1 total tags in treatment: 2307946 INFO @ Sat, 15 Jan 2022 21:26:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:26:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:26:55: #1 tags after filtering in treatment: 1783251 INFO @ Sat, 15 Jan 2022 21:26:55: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:26:55: #1 finished! INFO @ Sat, 15 Jan 2022 21:26:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:26:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:26:55: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 21:26:55: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:26:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:26:56: 1000000 INFO @ Sat, 15 Jan 2022 21:27:03: 2000000 INFO @ Sat, 15 Jan 2022 21:27:09: 3000000 INFO @ Sat, 15 Jan 2022 21:27:15: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:27:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:27:20: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:27:20: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:27:22: 5000000 INFO @ Sat, 15 Jan 2022 21:27:25: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:27:25: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:27:25: #1 total tags in treatment: 2307946 INFO @ Sat, 15 Jan 2022 21:27:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:27:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:27:25: #1 tags after filtering in treatment: 1783251 INFO @ Sat, 15 Jan 2022 21:27:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:27:25: #1 finished! INFO @ Sat, 15 Jan 2022 21:27:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:27:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:27:25: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 21:27:25: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:27:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 20 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:27:26: 1000000 INFO @ Sat, 15 Jan 2022 21:27:32: 2000000 INFO @ Sat, 15 Jan 2022 21:27:38: 3000000 INFO @ Sat, 15 Jan 2022 21:27:44: 4000000 INFO @ Sat, 15 Jan 2022 21:27:50: 5000000 INFO @ Sat, 15 Jan 2022 21:27:53: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 21:27:53: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 21:27:53: #1 total tags in treatment: 2307946 INFO @ Sat, 15 Jan 2022 21:27:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:27:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:27:53: #1 tags after filtering in treatment: 1783251 INFO @ Sat, 15 Jan 2022 21:27:53: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:27:53: #1 finished! INFO @ Sat, 15 Jan 2022 21:27:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:27:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:27:53: #2 number of paired peaks: 25 WARNING @ Sat, 15 Jan 2022 21:27:53: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:27:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399023/SRX9399023.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。