Job ID = 14521617 SRX = SRX9399009 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3335042 spots for SRR12935291/SRR12935291.sra Written 3335042 spots for SRR12935291/SRR12935291.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:43 3335042 reads; of these: 3335042 (100.00%) were paired; of these: 571187 (17.13%) aligned concordantly 0 times 2369536 (71.05%) aligned concordantly exactly 1 time 394319 (11.82%) aligned concordantly >1 times ---- 571187 pairs aligned concordantly 0 times; of these: 140130 (24.53%) aligned discordantly 1 time ---- 431057 pairs aligned 0 times concordantly or discordantly; of these: 862114 mates make up the pairs; of these: 713362 (82.75%) aligned 0 times 91636 (10.63%) aligned exactly 1 time 57116 (6.63%) aligned >1 times 89.31% overall alignment rate Time searching: 00:01:43 Overall time: 00:01:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 262293 / 2832743 = 0.0926 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:16:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:16:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:16:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:16:54: 1000000 INFO @ Sat, 15 Jan 2022 21:16:59: 2000000 INFO @ Sat, 15 Jan 2022 21:17:03: 3000000 INFO @ Sat, 15 Jan 2022 21:17:08: 4000000 INFO @ Sat, 15 Jan 2022 21:17:13: 5000000 INFO @ Sat, 15 Jan 2022 21:17:15: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:17:15: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:17:15: #1 total tags in treatment: 2505720 INFO @ Sat, 15 Jan 2022 21:17:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:17:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:17:15: #1 tags after filtering in treatment: 1938516 INFO @ Sat, 15 Jan 2022 21:17:15: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:17:15: #1 finished! INFO @ Sat, 15 Jan 2022 21:17:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:17:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:17:16: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 21:17:16: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:17:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:17:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:17:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:17:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:17:25: 1000000 INFO @ Sat, 15 Jan 2022 21:17:30: 2000000 INFO @ Sat, 15 Jan 2022 21:17:35: 3000000 INFO @ Sat, 15 Jan 2022 21:17:40: 4000000 INFO @ Sat, 15 Jan 2022 21:17:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:17:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:17:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:17:48: #1 total tags in treatment: 2505720 INFO @ Sat, 15 Jan 2022 21:17:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:17:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:17:48: #1 tags after filtering in treatment: 1938516 INFO @ Sat, 15 Jan 2022 21:17:48: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:17:48: #1 finished! INFO @ Sat, 15 Jan 2022 21:17:48: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:17:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:17:48: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 21:17:48: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:17:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:17:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:17:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:17:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:17:54: 1000000 INFO @ Sat, 15 Jan 2022 21:17:59: 2000000 INFO @ Sat, 15 Jan 2022 21:18:03: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:18:08: 4000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:18:14: 5000000 INFO @ Sat, 15 Jan 2022 21:18:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:18:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:18:16: #1 total tags in treatment: 2505720 INFO @ Sat, 15 Jan 2022 21:18:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:18:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:18:16: #1 tags after filtering in treatment: 1938516 INFO @ Sat, 15 Jan 2022 21:18:16: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 21:18:16: #1 finished! INFO @ Sat, 15 Jan 2022 21:18:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:18:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:18:16: #2 number of paired peaks: 24 WARNING @ Sat, 15 Jan 2022 21:18:16: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:18:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9399009/SRX9399009.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling