Job ID = 14519782 SRX = SRX9390802 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 2355772 spots for SRR12926697/SRR12926697.sra Written 2355772 spots for SRR12926697/SRR12926697.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:53 2355772 reads; of these: 2355772 (100.00%) were paired; of these: 1146112 (48.65%) aligned concordantly 0 times 838043 (35.57%) aligned concordantly exactly 1 time 371617 (15.77%) aligned concordantly >1 times ---- 1146112 pairs aligned concordantly 0 times; of these: 137001 (11.95%) aligned discordantly 1 time ---- 1009111 pairs aligned 0 times concordantly or discordantly; of these: 2018222 mates make up the pairs; of these: 882181 (43.71%) aligned 0 times 279304 (13.84%) aligned exactly 1 time 856737 (42.45%) aligned >1 times 81.28% overall alignment rate Time searching: 00:01:53 Overall time: 00:01:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 319079 / 1340107 = 0.2381 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:47:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:47:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:47:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:48:00: 1000000 INFO @ Sat, 15 Jan 2022 17:48:05: 2000000 INFO @ Sat, 15 Jan 2022 17:48:10: 3000000 INFO @ Sat, 15 Jan 2022 17:48:10: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 17:48:10: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 17:48:10: #1 total tags in treatment: 914165 INFO @ Sat, 15 Jan 2022 17:48:10: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:10: #1 tags after filtering in treatment: 690271 INFO @ Sat, 15 Jan 2022 17:48:10: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:48:10: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:10: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:11: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 17:48:11: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:48:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:48:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:48:31: 1000000 INFO @ Sat, 15 Jan 2022 17:48:36: 2000000 INFO @ Sat, 15 Jan 2022 17:48:43: 3000000 INFO @ Sat, 15 Jan 2022 17:48:44: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 17:48:44: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 17:48:44: #1 total tags in treatment: 914165 INFO @ Sat, 15 Jan 2022 17:48:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:48:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:48:44: #1 tags after filtering in treatment: 690271 INFO @ Sat, 15 Jan 2022 17:48:44: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:48:44: #1 finished! INFO @ Sat, 15 Jan 2022 17:48:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:48:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:48:44: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 17:48:44: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:48:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:48:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:48:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:49:01: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:49:07: 2000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:49:14: 3000000 INFO @ Sat, 15 Jan 2022 17:49:15: #1 tag size is determined as 70 bps INFO @ Sat, 15 Jan 2022 17:49:15: #1 tag size = 70 INFO @ Sat, 15 Jan 2022 17:49:15: #1 total tags in treatment: 914165 INFO @ Sat, 15 Jan 2022 17:49:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:49:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:49:15: #1 tags after filtering in treatment: 690271 INFO @ Sat, 15 Jan 2022 17:49:15: #1 Redundant rate of treatment: 0.24 INFO @ Sat, 15 Jan 2022 17:49:15: #1 finished! INFO @ Sat, 15 Jan 2022 17:49:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:49:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:49:15: #2 number of paired peaks: 50 WARNING @ Sat, 15 Jan 2022 17:49:15: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:49:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390802/SRX9390802.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling