Job ID = 14520120 SRX = SRX9390792 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3873404 spots for SRR12926687/SRR12926687.sra Written 3873404 spots for SRR12926687/SRR12926687.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:08 3873404 reads; of these: 3873404 (100.00%) were paired; of these: 2408247 (62.17%) aligned concordantly 0 times 889856 (22.97%) aligned concordantly exactly 1 time 575301 (14.85%) aligned concordantly >1 times ---- 2408247 pairs aligned concordantly 0 times; of these: 254635 (10.57%) aligned discordantly 1 time ---- 2153612 pairs aligned 0 times concordantly or discordantly; of these: 4307224 mates make up the pairs; of these: 1904431 (44.21%) aligned 0 times 573095 (13.31%) aligned exactly 1 time 1829698 (42.48%) aligned >1 times 75.42% overall alignment rate Time searching: 00:03:08 Overall time: 00:03:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 537944 / 1707827 = 0.3150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:23: 1000000 INFO @ Sat, 15 Jan 2022 18:27:28: 2000000 INFO @ Sat, 15 Jan 2022 18:27:33: 3000000 INFO @ Sat, 15 Jan 2022 18:27:37: 4000000 INFO @ Sat, 15 Jan 2022 18:27:41: #1 tag size is determined as 90 bps INFO @ Sat, 15 Jan 2022 18:27:41: #1 tag size = 90 INFO @ Sat, 15 Jan 2022 18:27:41: #1 total tags in treatment: 987319 INFO @ Sat, 15 Jan 2022 18:27:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:27:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:27:42: #1 tags after filtering in treatment: 687625 INFO @ Sat, 15 Jan 2022 18:27:42: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:27:42: #1 finished! INFO @ Sat, 15 Jan 2022 18:27:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:27:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:27:42: #2 number of paired peaks: 82 WARNING @ Sat, 15 Jan 2022 18:27:42: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:27:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:27:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:27:47: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:27:47: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:27:53: 1000000 INFO @ Sat, 15 Jan 2022 18:27:58: 2000000 INFO @ Sat, 15 Jan 2022 18:28:03: 3000000 INFO @ Sat, 15 Jan 2022 18:28:08: 4000000 INFO @ Sat, 15 Jan 2022 18:28:12: #1 tag size is determined as 90 bps INFO @ Sat, 15 Jan 2022 18:28:12: #1 tag size = 90 INFO @ Sat, 15 Jan 2022 18:28:12: #1 total tags in treatment: 987319 INFO @ Sat, 15 Jan 2022 18:28:12: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:12: #1 tags after filtering in treatment: 687625 INFO @ Sat, 15 Jan 2022 18:28:12: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:28:12: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:12: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:12: #2 number of paired peaks: 82 WARNING @ Sat, 15 Jan 2022 18:28:12: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 18:28:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 18:28:17: #1 read tag files... INFO @ Sat, 15 Jan 2022 18:28:17: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 18:28:23: 1000000 INFO @ Sat, 15 Jan 2022 18:28:28: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 18:28:32: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 18:28:37: 4000000 INFO @ Sat, 15 Jan 2022 18:28:41: #1 tag size is determined as 90 bps INFO @ Sat, 15 Jan 2022 18:28:41: #1 tag size = 90 INFO @ Sat, 15 Jan 2022 18:28:41: #1 total tags in treatment: 987319 INFO @ Sat, 15 Jan 2022 18:28:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 18:28:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 18:28:41: #1 tags after filtering in treatment: 687625 INFO @ Sat, 15 Jan 2022 18:28:41: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 18:28:41: #1 finished! INFO @ Sat, 15 Jan 2022 18:28:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 18:28:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 18:28:41: #2 number of paired peaks: 82 WARNING @ Sat, 15 Jan 2022 18:28:41: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 18:28:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9390792/SRX9390792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling