Job ID = 14520629 SRX = SRX9349775 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7590795 spots for SRR12883919/SRR12883919.sra Written 7590795 spots for SRR12883919/SRR12883919.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:43 7590795 reads; of these: 7590795 (100.00%) were paired; of these: 427605 (5.63%) aligned concordantly 0 times 6423482 (84.62%) aligned concordantly exactly 1 time 739708 (9.74%) aligned concordantly >1 times ---- 427605 pairs aligned concordantly 0 times; of these: 182101 (42.59%) aligned discordantly 1 time ---- 245504 pairs aligned 0 times concordantly or discordantly; of these: 491008 mates make up the pairs; of these: 380489 (77.49%) aligned 0 times 59394 (12.10%) aligned exactly 1 time 51125 (10.41%) aligned >1 times 97.49% overall alignment rate Time searching: 00:06:43 Overall time: 00:06:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 586096 / 7313316 = 0.0801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:41:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:41:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:41:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:31: 1000000 INFO @ Sat, 15 Jan 2022 19:41:36: 2000000 INFO @ Sat, 15 Jan 2022 19:41:42: 3000000 INFO @ Sat, 15 Jan 2022 19:41:47: 4000000 INFO @ Sat, 15 Jan 2022 19:41:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:41:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:41:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:41:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:41:58: 6000000 INFO @ Sat, 15 Jan 2022 19:42:02: 1000000 INFO @ Sat, 15 Jan 2022 19:42:03: 7000000 INFO @ Sat, 15 Jan 2022 19:42:07: 2000000 INFO @ Sat, 15 Jan 2022 19:42:09: 8000000 INFO @ Sat, 15 Jan 2022 19:42:13: 3000000 INFO @ Sat, 15 Jan 2022 19:42:15: 9000000 INFO @ Sat, 15 Jan 2022 19:42:19: 4000000 INFO @ Sat, 15 Jan 2022 19:42:21: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:42:25: 5000000 INFO @ Sat, 15 Jan 2022 19:42:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:42:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:42:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:42:27: 11000000 INFO @ Sat, 15 Jan 2022 19:42:31: 6000000 INFO @ Sat, 15 Jan 2022 19:42:32: 1000000 INFO @ Sat, 15 Jan 2022 19:42:33: 12000000 INFO @ Sat, 15 Jan 2022 19:42:37: 7000000 INFO @ Sat, 15 Jan 2022 19:42:37: 2000000 INFO @ Sat, 15 Jan 2022 19:42:38: 13000000 INFO @ Sat, 15 Jan 2022 19:42:42: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:42:42: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:42:42: #1 total tags in treatment: 6581234 INFO @ Sat, 15 Jan 2022 19:42:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:42:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:42:42: #1 tags after filtering in treatment: 4214978 INFO @ Sat, 15 Jan 2022 19:42:42: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:42:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:42:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:42:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:42:43: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 19:42:43: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:42:43: Process for pairing-model is terminated! INFO @ Sat, 15 Jan 2022 19:42:43: 8000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:42:43: 3000000 INFO @ Sat, 15 Jan 2022 19:42:48: 9000000 INFO @ Sat, 15 Jan 2022 19:42:49: 4000000 INFO @ Sat, 15 Jan 2022 19:42:54: 10000000 INFO @ Sat, 15 Jan 2022 19:42:55: 5000000 INFO @ Sat, 15 Jan 2022 19:43:00: 11000000 INFO @ Sat, 15 Jan 2022 19:43:00: 6000000 INFO @ Sat, 15 Jan 2022 19:43:05: 12000000 INFO @ Sat, 15 Jan 2022 19:43:06: 7000000 INFO @ Sat, 15 Jan 2022 19:43:11: 13000000 INFO @ Sat, 15 Jan 2022 19:43:12: 8000000 INFO @ Sat, 15 Jan 2022 19:43:15: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:43:15: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:43:15: #1 total tags in treatment: 6581234 INFO @ Sat, 15 Jan 2022 19:43:15: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:15: #1 tags after filtering in treatment: 4214978 INFO @ Sat, 15 Jan 2022 19:43:15: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:43:15: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:15: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:15: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 19:43:15: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:43:17: 9000000 INFO @ Sat, 15 Jan 2022 19:43:23: 10000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:43:28: 11000000 INFO @ Sat, 15 Jan 2022 19:43:33: 12000000 INFO @ Sat, 15 Jan 2022 19:43:38: 13000000 INFO @ Sat, 15 Jan 2022 19:43:42: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:43:42: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:43:42: #1 total tags in treatment: 6581234 INFO @ Sat, 15 Jan 2022 19:43:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:43:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:43:42: #1 tags after filtering in treatment: 4214978 INFO @ Sat, 15 Jan 2022 19:43:42: #1 Redundant rate of treatment: 0.36 INFO @ Sat, 15 Jan 2022 19:43:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:43:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:43:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:43:42: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 19:43:42: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:43:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349775/SRX9349775.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling