Job ID = 14520626 SRX = SRX9349772 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10709037 spots for SRR12883916/SRR12883916.sra Written 10709037 spots for SRR12883916/SRR12883916.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:40 10709037 reads; of these: 10709037 (100.00%) were paired; of these: 808844 (7.55%) aligned concordantly 0 times 9259060 (86.46%) aligned concordantly exactly 1 time 641133 (5.99%) aligned concordantly >1 times ---- 808844 pairs aligned concordantly 0 times; of these: 489044 (60.46%) aligned discordantly 1 time ---- 319800 pairs aligned 0 times concordantly or discordantly; of these: 639600 mates make up the pairs; of these: 487242 (76.18%) aligned 0 times 82814 (12.95%) aligned exactly 1 time 69544 (10.87%) aligned >1 times 97.73% overall alignment rate Time searching: 00:09:40 Overall time: 00:09:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1131859 / 10312115 = 0.1098 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:51: 1000000 INFO @ Sat, 15 Jan 2022 19:46:57: 2000000 INFO @ Sat, 15 Jan 2022 19:47:04: 3000000 INFO @ Sat, 15 Jan 2022 19:47:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:14: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:14: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:17: 5000000 INFO @ Sat, 15 Jan 2022 19:47:21: 1000000 INFO @ Sat, 15 Jan 2022 19:47:24: 6000000 INFO @ Sat, 15 Jan 2022 19:47:28: 2000000 INFO @ Sat, 15 Jan 2022 19:47:31: 7000000 INFO @ Sat, 15 Jan 2022 19:47:35: 3000000 INFO @ Sat, 15 Jan 2022 19:47:38: 8000000 INFO @ Sat, 15 Jan 2022 19:47:42: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:44: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:44: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:45: 9000000 INFO @ Sat, 15 Jan 2022 19:47:49: 5000000 INFO @ Sat, 15 Jan 2022 19:47:50: 1000000 INFO @ Sat, 15 Jan 2022 19:47:52: 10000000 INFO @ Sat, 15 Jan 2022 19:47:56: 2000000 INFO @ Sat, 15 Jan 2022 19:47:56: 6000000 INFO @ Sat, 15 Jan 2022 19:47:58: 11000000 INFO @ Sat, 15 Jan 2022 19:48:02: 3000000 INFO @ Sat, 15 Jan 2022 19:48:03: 7000000 INFO @ Sat, 15 Jan 2022 19:48:05: 12000000 INFO @ Sat, 15 Jan 2022 19:48:08: 4000000 INFO @ Sat, 15 Jan 2022 19:48:10: 8000000 INFO @ Sat, 15 Jan 2022 19:48:12: 13000000 INFO @ Sat, 15 Jan 2022 19:48:14: 5000000 INFO @ Sat, 15 Jan 2022 19:48:17: 9000000 INFO @ Sat, 15 Jan 2022 19:48:19: 14000000 INFO @ Sat, 15 Jan 2022 19:48:19: 6000000 INFO @ Sat, 15 Jan 2022 19:48:24: 10000000 INFO @ Sat, 15 Jan 2022 19:48:25: 7000000 INFO @ Sat, 15 Jan 2022 19:48:26: 15000000 INFO @ Sat, 15 Jan 2022 19:48:30: 11000000 INFO @ Sat, 15 Jan 2022 19:48:31: 8000000 INFO @ Sat, 15 Jan 2022 19:48:33: 16000000 INFO @ Sat, 15 Jan 2022 19:48:36: 12000000 INFO @ Sat, 15 Jan 2022 19:48:37: 9000000 INFO @ Sat, 15 Jan 2022 19:48:39: 17000000 INFO @ Sat, 15 Jan 2022 19:48:43: 10000000 INFO @ Sat, 15 Jan 2022 19:48:43: 13000000 INFO @ Sat, 15 Jan 2022 19:48:46: 18000000 INFO @ Sat, 15 Jan 2022 19:48:49: 11000000 INFO @ Sat, 15 Jan 2022 19:48:49: 14000000 INFO @ Sat, 15 Jan 2022 19:48:50: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:48:50: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:48:50: #1 total tags in treatment: 8791887 INFO @ Sat, 15 Jan 2022 19:48:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:50: #1 tags after filtering in treatment: 5069237 INFO @ Sat, 15 Jan 2022 19:48:50: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:48:50: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:48:55: 12000000 INFO @ Sat, 15 Jan 2022 19:48:56: 15000000 INFO @ Sat, 15 Jan 2022 19:49:01: 13000000 INFO @ Sat, 15 Jan 2022 19:49:03: 16000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:49:07: 14000000 INFO @ Sat, 15 Jan 2022 19:49:09: 17000000 INFO @ Sat, 15 Jan 2022 19:49:12: 15000000 INFO @ Sat, 15 Jan 2022 19:49:16: 18000000 INFO @ Sat, 15 Jan 2022 19:49:18: 16000000 INFO @ Sat, 15 Jan 2022 19:49:20: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:49:20: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:49:20: #1 total tags in treatment: 8791887 INFO @ Sat, 15 Jan 2022 19:49:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:20: #1 tags after filtering in treatment: 5069237 INFO @ Sat, 15 Jan 2022 19:49:20: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:49:20: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:20: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:49:24: 17000000 INFO @ Sat, 15 Jan 2022 19:49:29: 18000000 INFO @ Sat, 15 Jan 2022 19:49:32: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:49:32: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:49:32: #1 total tags in treatment: 8791887 INFO @ Sat, 15 Jan 2022 19:49:32: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:49:32: #1 tags after filtering in treatment: 5069237 INFO @ Sat, 15 Jan 2022 19:49:32: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 19:49:32: #1 finished! INFO @ Sat, 15 Jan 2022 19:49:32: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:49:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:49:33: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:49:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:49:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349772/SRX9349772.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling