Job ID = 14520625 SRX = SRX9349771 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9520445 spots for SRR12883915/SRR12883915.sra Written 9520445 spots for SRR12883915/SRR12883915.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:44 9520445 reads; of these: 9520445 (100.00%) were paired; of these: 573784 (6.03%) aligned concordantly 0 times 8013818 (84.17%) aligned concordantly exactly 1 time 932843 (9.80%) aligned concordantly >1 times ---- 573784 pairs aligned concordantly 0 times; of these: 291613 (50.82%) aligned discordantly 1 time ---- 282171 pairs aligned 0 times concordantly or discordantly; of these: 564342 mates make up the pairs; of these: 424787 (75.27%) aligned 0 times 65611 (11.63%) aligned exactly 1 time 73944 (13.10%) aligned >1 times 97.77% overall alignment rate Time searching: 00:08:44 Overall time: 00:08:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1136555 / 9185916 = 0.1237 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:11: 1000000 INFO @ Sat, 15 Jan 2022 19:45:16: 2000000 INFO @ Sat, 15 Jan 2022 19:45:22: 3000000 INFO @ Sat, 15 Jan 2022 19:45:27: 4000000 INFO @ Sat, 15 Jan 2022 19:45:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:39: 6000000 INFO @ Sat, 15 Jan 2022 19:45:42: 1000000 INFO @ Sat, 15 Jan 2022 19:45:45: 7000000 INFO @ Sat, 15 Jan 2022 19:45:48: 2000000 INFO @ Sat, 15 Jan 2022 19:45:51: 8000000 INFO @ Sat, 15 Jan 2022 19:45:54: 3000000 INFO @ Sat, 15 Jan 2022 19:45:57: 9000000 INFO @ Sat, 15 Jan 2022 19:46:00: 4000000 INFO @ Sat, 15 Jan 2022 19:46:03: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:06: 5000000 INFO @ Sat, 15 Jan 2022 19:46:09: 11000000 INFO @ Sat, 15 Jan 2022 19:46:12: 6000000 INFO @ Sat, 15 Jan 2022 19:46:12: 1000000 INFO @ Sat, 15 Jan 2022 19:46:15: 12000000 INFO @ Sat, 15 Jan 2022 19:46:18: 7000000 INFO @ Sat, 15 Jan 2022 19:46:18: 2000000 INFO @ Sat, 15 Jan 2022 19:46:21: 13000000 INFO @ Sat, 15 Jan 2022 19:46:24: 8000000 INFO @ Sat, 15 Jan 2022 19:46:24: 3000000 INFO @ Sat, 15 Jan 2022 19:46:27: 14000000 INFO @ Sat, 15 Jan 2022 19:46:30: 9000000 INFO @ Sat, 15 Jan 2022 19:46:30: 4000000 INFO @ Sat, 15 Jan 2022 19:46:33: 15000000 INFO @ Sat, 15 Jan 2022 19:46:36: 10000000 INFO @ Sat, 15 Jan 2022 19:46:36: 5000000 INFO @ Sat, 15 Jan 2022 19:46:39: 16000000 INFO @ Sat, 15 Jan 2022 19:46:41: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:46:41: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:46:41: #1 total tags in treatment: 7821795 INFO @ Sat, 15 Jan 2022 19:46:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:46:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:46:41: #1 tags after filtering in treatment: 4426994 INFO @ Sat, 15 Jan 2022 19:46:41: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:46:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:46:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:46:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:46:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:46:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:46:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 15 Jan 2022 19:46:42: 11000000 INFO @ Sat, 15 Jan 2022 19:46:42: 6000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:46:48: 12000000 INFO @ Sat, 15 Jan 2022 19:46:48: 7000000 INFO @ Sat, 15 Jan 2022 19:46:54: 8000000 INFO @ Sat, 15 Jan 2022 19:46:54: 13000000 INFO @ Sat, 15 Jan 2022 19:47:00: 9000000 INFO @ Sat, 15 Jan 2022 19:47:00: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:47:06: 10000000 INFO @ Sat, 15 Jan 2022 19:47:06: 15000000 INFO @ Sat, 15 Jan 2022 19:47:12: 11000000 INFO @ Sat, 15 Jan 2022 19:47:12: 16000000 INFO @ Sat, 15 Jan 2022 19:47:14: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:47:14: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:47:14: #1 total tags in treatment: 7821795 INFO @ Sat, 15 Jan 2022 19:47:14: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:14: #1 tags after filtering in treatment: 4426994 INFO @ Sat, 15 Jan 2022 19:47:14: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:47:14: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:14: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:47:18: 12000000 INFO @ Sat, 15 Jan 2022 19:47:24: 13000000 INFO @ Sat, 15 Jan 2022 19:47:29: 14000000 INFO @ Sat, 15 Jan 2022 19:47:35: 15000000 INFO @ Sat, 15 Jan 2022 19:47:41: 16000000 INFO @ Sat, 15 Jan 2022 19:47:43: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:47:43: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:47:43: #1 total tags in treatment: 7821795 INFO @ Sat, 15 Jan 2022 19:47:43: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:43: #1 tags after filtering in treatment: 4426994 INFO @ Sat, 15 Jan 2022 19:47:43: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:47:43: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349771/SRX9349771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling