Job ID = 14520624 SRX = SRX9349770 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9619580 spots for SRR12883914/SRR12883914.sra Written 9619580 spots for SRR12883914/SRR12883914.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:31 9619580 reads; of these: 9619580 (100.00%) were paired; of these: 788558 (8.20%) aligned concordantly 0 times 8192127 (85.16%) aligned concordantly exactly 1 time 638895 (6.64%) aligned concordantly >1 times ---- 788558 pairs aligned concordantly 0 times; of these: 475590 (60.31%) aligned discordantly 1 time ---- 312968 pairs aligned 0 times concordantly or discordantly; of these: 625936 mates make up the pairs; of these: 467482 (74.69%) aligned 0 times 83676 (13.37%) aligned exactly 1 time 74778 (11.95%) aligned >1 times 97.57% overall alignment rate Time searching: 00:08:31 Overall time: 00:08:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1091808 / 9230783 = 0.1183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:11: 1000000 INFO @ Sat, 15 Jan 2022 19:46:16: 2000000 INFO @ Sat, 15 Jan 2022 19:46:21: 3000000 INFO @ Sat, 15 Jan 2022 19:46:27: 4000000 INFO @ Sat, 15 Jan 2022 19:46:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:46:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:46:35: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:46:35: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:46:37: 6000000 INFO @ Sat, 15 Jan 2022 19:46:42: 1000000 INFO @ Sat, 15 Jan 2022 19:46:43: 7000000 INFO @ Sat, 15 Jan 2022 19:46:48: 8000000 INFO @ Sat, 15 Jan 2022 19:46:49: 2000000 INFO @ Sat, 15 Jan 2022 19:46:54: 9000000 INFO @ Sat, 15 Jan 2022 19:46:56: 3000000 INFO @ Sat, 15 Jan 2022 19:46:59: 10000000 INFO @ Sat, 15 Jan 2022 19:47:03: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:47:05: 11000000 INFO @ Sat, 15 Jan 2022 19:47:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:47:05: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:47:05: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:47:09: 5000000 INFO @ Sat, 15 Jan 2022 19:47:11: 12000000 INFO @ Sat, 15 Jan 2022 19:47:11: 1000000 INFO @ Sat, 15 Jan 2022 19:47:15: 6000000 INFO @ Sat, 15 Jan 2022 19:47:16: 13000000 INFO @ Sat, 15 Jan 2022 19:47:17: 2000000 INFO @ Sat, 15 Jan 2022 19:47:22: 7000000 INFO @ Sat, 15 Jan 2022 19:47:22: 14000000 INFO @ Sat, 15 Jan 2022 19:47:23: 3000000 INFO @ Sat, 15 Jan 2022 19:47:27: 8000000 INFO @ Sat, 15 Jan 2022 19:47:28: 15000000 INFO @ Sat, 15 Jan 2022 19:47:29: 4000000 INFO @ Sat, 15 Jan 2022 19:47:33: 9000000 INFO @ Sat, 15 Jan 2022 19:47:33: 16000000 INFO @ Sat, 15 Jan 2022 19:47:34: 5000000 INFO @ Sat, 15 Jan 2022 19:47:37: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:47:37: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:47:37: #1 total tags in treatment: 7763694 INFO @ Sat, 15 Jan 2022 19:47:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:37: #1 tags after filtering in treatment: 4421005 INFO @ Sat, 15 Jan 2022 19:47:37: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:47:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:39: 10000000 INFO @ Sat, 15 Jan 2022 19:47:40: 6000000 INFO @ Sat, 15 Jan 2022 19:47:45: 11000000 INFO @ Sat, 15 Jan 2022 19:47:46: 7000000 INFO @ Sat, 15 Jan 2022 19:47:51: 8000000 INFO @ Sat, 15 Jan 2022 19:47:51: 12000000 INFO @ Sat, 15 Jan 2022 19:47:57: 9000000 INFO @ Sat, 15 Jan 2022 19:47:58: 13000000 INFO @ Sat, 15 Jan 2022 19:48:03: 10000000 INFO @ Sat, 15 Jan 2022 19:48:05: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:48:08: 11000000 INFO @ Sat, 15 Jan 2022 19:48:11: 15000000 INFO @ Sat, 15 Jan 2022 19:48:14: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:48:18: 16000000 INFO @ Sat, 15 Jan 2022 19:48:19: 13000000 INFO @ Sat, 15 Jan 2022 19:48:22: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:48:22: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:48:22: #1 total tags in treatment: 7763694 INFO @ Sat, 15 Jan 2022 19:48:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:22: #1 tags after filtering in treatment: 4421005 INFO @ Sat, 15 Jan 2022 19:48:22: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:48:22: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:22: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:48:25: 14000000 INFO @ Sat, 15 Jan 2022 19:48:30: 15000000 INFO @ Sat, 15 Jan 2022 19:48:35: 16000000 INFO @ Sat, 15 Jan 2022 19:48:39: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 19:48:39: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 19:48:39: #1 total tags in treatment: 7763694 INFO @ Sat, 15 Jan 2022 19:48:39: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:39: #1 tags after filtering in treatment: 4421005 INFO @ Sat, 15 Jan 2022 19:48:39: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 19:48:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349770/SRX9349770.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling