Job ID = 14520602 SRX = SRX9349768 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10767679 spots for SRR12883912/SRR12883912.sra Written 10767679 spots for SRR12883912/SRR12883912.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:38 10767679 reads; of these: 10767679 (100.00%) were paired; of these: 821256 (7.63%) aligned concordantly 0 times 9273219 (86.12%) aligned concordantly exactly 1 time 673204 (6.25%) aligned concordantly >1 times ---- 821256 pairs aligned concordantly 0 times; of these: 467057 (56.87%) aligned discordantly 1 time ---- 354199 pairs aligned 0 times concordantly or discordantly; of these: 708398 mates make up the pairs; of these: 532122 (75.12%) aligned 0 times 103167 (14.56%) aligned exactly 1 time 73109 (10.32%) aligned >1 times 97.53% overall alignment rate Time searching: 00:14:38 Overall time: 00:14:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1216093 / 10339273 = 0.1176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:59:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:59:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:59:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:59:22: 1000000 INFO @ Sat, 15 Jan 2022 19:59:33: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:59:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:59:39: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:59:39: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:59:44: 3000000 INFO @ Sat, 15 Jan 2022 19:59:52: 1000000 INFO @ Sat, 15 Jan 2022 19:59:57: 4000000 INFO @ Sat, 15 Jan 2022 20:00:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:00:09: 5000000 INFO @ Sat, 15 Jan 2022 20:00:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:00:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:00:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:00:17: 3000000 INFO @ Sat, 15 Jan 2022 20:00:20: 1000000 INFO @ Sat, 15 Jan 2022 20:00:21: 6000000 INFO @ Sat, 15 Jan 2022 20:00:30: 4000000 INFO @ Sat, 15 Jan 2022 20:00:30: 2000000 INFO @ Sat, 15 Jan 2022 20:00:32: 7000000 INFO @ Sat, 15 Jan 2022 20:00:40: 3000000 INFO @ Sat, 15 Jan 2022 20:00:43: 5000000 INFO @ Sat, 15 Jan 2022 20:00:44: 8000000 INFO @ Sat, 15 Jan 2022 20:00:50: 4000000 INFO @ Sat, 15 Jan 2022 20:00:55: 6000000 INFO @ Sat, 15 Jan 2022 20:00:57: 9000000 INFO @ Sat, 15 Jan 2022 20:01:01: 5000000 INFO @ Sat, 15 Jan 2022 20:01:08: 7000000 INFO @ Sat, 15 Jan 2022 20:01:09: 10000000 INFO @ Sat, 15 Jan 2022 20:01:12: 6000000 INFO @ Sat, 15 Jan 2022 20:01:20: 8000000 INFO @ Sat, 15 Jan 2022 20:01:21: 11000000 INFO @ Sat, 15 Jan 2022 20:01:21: 7000000 INFO @ Sat, 15 Jan 2022 20:01:31: 8000000 INFO @ Sat, 15 Jan 2022 20:01:33: 12000000 INFO @ Sat, 15 Jan 2022 20:01:34: 9000000 INFO @ Sat, 15 Jan 2022 20:01:41: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:01:46: 13000000 INFO @ Sat, 15 Jan 2022 20:01:47: 10000000 INFO @ Sat, 15 Jan 2022 20:01:51: 10000000 INFO @ Sat, 15 Jan 2022 20:01:58: 14000000 INFO @ Sat, 15 Jan 2022 20:02:01: 11000000 INFO @ Sat, 15 Jan 2022 20:02:01: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:02:11: 12000000 INFO @ Sat, 15 Jan 2022 20:02:11: 15000000 INFO @ Sat, 15 Jan 2022 20:02:14: 12000000 INFO @ Sat, 15 Jan 2022 20:02:20: 13000000 INFO @ Sat, 15 Jan 2022 20:02:24: 16000000 INFO @ Sat, 15 Jan 2022 20:02:29: 13000000 INFO @ Sat, 15 Jan 2022 20:02:30: 14000000 INFO @ Sat, 15 Jan 2022 20:02:37: 17000000 INFO @ Sat, 15 Jan 2022 20:02:41: 15000000 INFO @ Sat, 15 Jan 2022 20:02:43: 14000000 INFO @ Sat, 15 Jan 2022 20:02:51: 18000000 INFO @ Sat, 15 Jan 2022 20:02:52: 16000000 INFO @ Sat, 15 Jan 2022 20:02:58: 15000000 INFO @ Sat, 15 Jan 2022 20:02:59: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 20:02:59: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 20:02:59: #1 total tags in treatment: 8755375 INFO @ Sat, 15 Jan 2022 20:02:59: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:02:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:02:59: #1 tags after filtering in treatment: 5064167 INFO @ Sat, 15 Jan 2022 20:02:59: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:02:59: #1 finished! INFO @ Sat, 15 Jan 2022 20:02:59: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:02:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:02:59: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:02:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:02:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:03:04: 17000000 INFO @ Sat, 15 Jan 2022 20:03:11: 16000000 INFO @ Sat, 15 Jan 2022 20:03:14: 18000000 INFO @ Sat, 15 Jan 2022 20:03:21: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 20:03:21: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 20:03:21: #1 total tags in treatment: 8755375 INFO @ Sat, 15 Jan 2022 20:03:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:03:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:03:21: #1 tags after filtering in treatment: 5064167 INFO @ Sat, 15 Jan 2022 20:03:21: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:03:21: #1 finished! INFO @ Sat, 15 Jan 2022 20:03:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:03:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:03:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:03:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:03:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:03:24: 17000000 INFO @ Sat, 15 Jan 2022 20:03:37: 18000000 INFO @ Sat, 15 Jan 2022 20:03:44: #1 tag size is determined as 101 bps INFO @ Sat, 15 Jan 2022 20:03:44: #1 tag size = 101 INFO @ Sat, 15 Jan 2022 20:03:44: #1 total tags in treatment: 8755375 INFO @ Sat, 15 Jan 2022 20:03:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:03:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:03:44: #1 tags after filtering in treatment: 5064167 INFO @ Sat, 15 Jan 2022 20:03:44: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 20:03:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:03:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:03:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:03:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:03:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:03:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9349768/SRX9349768.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling