Job ID = 14520238 SRX = SRX9346310 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8681794 spots for SRR12880348/SRR12880348.sra Written 8681794 spots for SRR12880348/SRR12880348.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:52 8681794 reads; of these: 8681794 (100.00%) were paired; of these: 260199 (3.00%) aligned concordantly 0 times 7687161 (88.54%) aligned concordantly exactly 1 time 734434 (8.46%) aligned concordantly >1 times ---- 260199 pairs aligned concordantly 0 times; of these: 60330 (23.19%) aligned discordantly 1 time ---- 199869 pairs aligned 0 times concordantly or discordantly; of these: 399738 mates make up the pairs; of these: 260052 (65.06%) aligned 0 times 115400 (28.87%) aligned exactly 1 time 24286 (6.08%) aligned >1 times 98.50% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 989867 / 8329175 = 0.1188 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:03:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:03:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:03:36: 1000000 INFO @ Sat, 15 Jan 2022 19:03:42: 2000000 INFO @ Sat, 15 Jan 2022 19:03:49: 3000000 INFO @ Sat, 15 Jan 2022 19:03:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:03:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:03:59: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:03:59: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:02: 5000000 INFO @ Sat, 15 Jan 2022 19:04:06: 1000000 INFO @ Sat, 15 Jan 2022 19:04:09: 6000000 INFO @ Sat, 15 Jan 2022 19:04:13: 2000000 INFO @ Sat, 15 Jan 2022 19:04:16: 7000000 INFO @ Sat, 15 Jan 2022 19:04:20: 3000000 INFO @ Sat, 15 Jan 2022 19:04:23: 8000000 INFO @ Sat, 15 Jan 2022 19:04:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:04:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:04:29: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:04:29: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:04:30: 9000000 INFO @ Sat, 15 Jan 2022 19:04:34: 5000000 INFO @ Sat, 15 Jan 2022 19:04:36: 1000000 INFO @ Sat, 15 Jan 2022 19:04:37: 10000000 INFO @ Sat, 15 Jan 2022 19:04:41: 6000000 INFO @ Sat, 15 Jan 2022 19:04:43: 2000000 INFO @ Sat, 15 Jan 2022 19:04:44: 11000000 INFO @ Sat, 15 Jan 2022 19:04:48: 7000000 INFO @ Sat, 15 Jan 2022 19:04:50: 3000000 INFO @ Sat, 15 Jan 2022 19:04:51: 12000000 INFO @ Sat, 15 Jan 2022 19:04:55: 8000000 INFO @ Sat, 15 Jan 2022 19:04:57: 4000000 INFO @ Sat, 15 Jan 2022 19:04:58: 13000000 INFO @ Sat, 15 Jan 2022 19:05:01: 9000000 INFO @ Sat, 15 Jan 2022 19:05:05: 5000000 INFO @ Sat, 15 Jan 2022 19:05:05: 14000000 INFO @ Sat, 15 Jan 2022 19:05:08: 10000000 INFO @ Sat, 15 Jan 2022 19:05:12: 6000000 INFO @ Sat, 15 Jan 2022 19:05:12: 15000000 INFO @ Sat, 15 Jan 2022 19:05:13: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:05:13: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:05:13: #1 total tags in treatment: 7432416 INFO @ Sat, 15 Jan 2022 19:05:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:13: #1 tags after filtering in treatment: 4449989 INFO @ Sat, 15 Jan 2022 19:05:13: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:05:13: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:05:15: 11000000 INFO @ Sat, 15 Jan 2022 19:05:19: 7000000 INFO @ Sat, 15 Jan 2022 19:05:22: 12000000 INFO @ Sat, 15 Jan 2022 19:05:25: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:05:29: 13000000 INFO @ Sat, 15 Jan 2022 19:05:32: 9000000 INFO @ Sat, 15 Jan 2022 19:05:37: 14000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:05:38: 10000000 INFO @ Sat, 15 Jan 2022 19:05:44: 15000000 INFO @ Sat, 15 Jan 2022 19:05:45: 11000000 INFO @ Sat, 15 Jan 2022 19:05:45: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:05:45: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:05:45: #1 total tags in treatment: 7432416 INFO @ Sat, 15 Jan 2022 19:05:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:05:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:05:45: #1 tags after filtering in treatment: 4449989 INFO @ Sat, 15 Jan 2022 19:05:45: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:05:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:05:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:05:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:05:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:05:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:05:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:05:50: 12000000 INFO @ Sat, 15 Jan 2022 19:05:56: 13000000 INFO @ Sat, 15 Jan 2022 19:06:02: 14000000 INFO @ Sat, 15 Jan 2022 19:06:07: 15000000 INFO @ Sat, 15 Jan 2022 19:06:08: #1 tag size is determined as 100 bps INFO @ Sat, 15 Jan 2022 19:06:08: #1 tag size = 100 INFO @ Sat, 15 Jan 2022 19:06:08: #1 total tags in treatment: 7432416 INFO @ Sat, 15 Jan 2022 19:06:08: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:06:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:06:08: #1 tags after filtering in treatment: 4449989 INFO @ Sat, 15 Jan 2022 19:06:08: #1 Redundant rate of treatment: 0.40 INFO @ Sat, 15 Jan 2022 19:06:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:06:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:06:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:06:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:06:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:06:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9346310/SRX9346310.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling