Job ID = 14520144 SRX = SRX9345661 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 17990179 spots for SRR12879685/SRR12879685.sra Written 17990179 spots for SRR12879685/SRR12879685.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:52 17990179 reads; of these: 17990179 (100.00%) were paired; of these: 1275457 (7.09%) aligned concordantly 0 times 13183928 (73.28%) aligned concordantly exactly 1 time 3530794 (19.63%) aligned concordantly >1 times ---- 1275457 pairs aligned concordantly 0 times; of these: 255402 (20.02%) aligned discordantly 1 time ---- 1020055 pairs aligned 0 times concordantly or discordantly; of these: 2040110 mates make up the pairs; of these: 1428532 (70.02%) aligned 0 times 366062 (17.94%) aligned exactly 1 time 245516 (12.03%) aligned >1 times 96.03% overall alignment rate Time searching: 00:20:52 Overall time: 00:20:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 517494 / 9743206 = 0.0531 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:05:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:05:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:05:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:05:55: 1000000 INFO @ Sat, 15 Jan 2022 19:06:04: 2000000 INFO @ Sat, 15 Jan 2022 19:06:13: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:06:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:06:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:06:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:06:24: 4000000 INFO @ Sat, 15 Jan 2022 19:06:27: 1000000 INFO @ Sat, 15 Jan 2022 19:06:35: 5000000 INFO @ Sat, 15 Jan 2022 19:06:36: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:06:45: 6000000 INFO @ Sat, 15 Jan 2022 19:06:46: 3000000 INFO @ Sat, 15 Jan 2022 19:06:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:06:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:06:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:06:56: 7000000 INFO @ Sat, 15 Jan 2022 19:06:57: 4000000 INFO @ Sat, 15 Jan 2022 19:06:58: 1000000 INFO @ Sat, 15 Jan 2022 19:07:07: 8000000 INFO @ Sat, 15 Jan 2022 19:07:09: 5000000 INFO @ Sat, 15 Jan 2022 19:07:10: 2000000 INFO @ Sat, 15 Jan 2022 19:07:19: 9000000 INFO @ Sat, 15 Jan 2022 19:07:20: 6000000 INFO @ Sat, 15 Jan 2022 19:07:21: 3000000 INFO @ Sat, 15 Jan 2022 19:07:30: 10000000 INFO @ Sat, 15 Jan 2022 19:07:32: 7000000 INFO @ Sat, 15 Jan 2022 19:07:33: 4000000 INFO @ Sat, 15 Jan 2022 19:07:42: 11000000 INFO @ Sat, 15 Jan 2022 19:07:43: 8000000 INFO @ Sat, 15 Jan 2022 19:07:45: 5000000 INFO @ Sat, 15 Jan 2022 19:07:54: 12000000 INFO @ Sat, 15 Jan 2022 19:07:55: 9000000 INFO @ Sat, 15 Jan 2022 19:07:56: 6000000 INFO @ Sat, 15 Jan 2022 19:08:05: 13000000 INFO @ Sat, 15 Jan 2022 19:08:06: 10000000 INFO @ Sat, 15 Jan 2022 19:08:08: 7000000 INFO @ Sat, 15 Jan 2022 19:08:17: 14000000 INFO @ Sat, 15 Jan 2022 19:08:18: 11000000 INFO @ Sat, 15 Jan 2022 19:08:20: 8000000 INFO @ Sat, 15 Jan 2022 19:08:28: 15000000 INFO @ Sat, 15 Jan 2022 19:08:29: 12000000 INFO @ Sat, 15 Jan 2022 19:08:31: 9000000 INFO @ Sat, 15 Jan 2022 19:08:39: 16000000 INFO @ Sat, 15 Jan 2022 19:08:40: 13000000 INFO @ Sat, 15 Jan 2022 19:08:43: 10000000 INFO @ Sat, 15 Jan 2022 19:08:51: 17000000 INFO @ Sat, 15 Jan 2022 19:08:52: 14000000 INFO @ Sat, 15 Jan 2022 19:08:55: 11000000 INFO @ Sat, 15 Jan 2022 19:09:02: 18000000 INFO @ Sat, 15 Jan 2022 19:09:03: 15000000 INFO @ Sat, 15 Jan 2022 19:09:06: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:09:14: 19000000 INFO @ Sat, 15 Jan 2022 19:09:15: 16000000 INFO @ Sat, 15 Jan 2022 19:09:18: 13000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:09:25: 20000000 INFO @ Sat, 15 Jan 2022 19:09:26: 17000000 INFO @ Sat, 15 Jan 2022 19:09:30: 14000000 INFO @ Sat, 15 Jan 2022 19:09:36: 21000000 INFO @ Sat, 15 Jan 2022 19:09:38: 18000000 INFO @ Sat, 15 Jan 2022 19:09:42: 15000000 INFO @ Sat, 15 Jan 2022 19:09:48: 22000000 INFO @ Sat, 15 Jan 2022 19:09:49: 19000000 INFO @ Sat, 15 Jan 2022 19:09:54: 16000000 INFO @ Sat, 15 Jan 2022 19:09:59: 23000000 INFO @ Sat, 15 Jan 2022 19:10:01: 20000000 INFO @ Sat, 15 Jan 2022 19:10:06: 17000000 INFO @ Sat, 15 Jan 2022 19:10:10: 24000000 INFO @ Sat, 15 Jan 2022 19:10:12: 21000000 INFO @ Sat, 15 Jan 2022 19:10:18: 18000000 INFO @ Sat, 15 Jan 2022 19:10:22: 25000000 INFO @ Sat, 15 Jan 2022 19:10:23: 22000000 INFO @ Sat, 15 Jan 2022 19:10:30: 19000000 INFO @ Sat, 15 Jan 2022 19:10:33: 26000000 INFO @ Sat, 15 Jan 2022 19:10:35: 23000000 INFO @ Sat, 15 Jan 2022 19:10:41: 20000000 INFO @ Sat, 15 Jan 2022 19:10:45: 27000000 INFO @ Sat, 15 Jan 2022 19:10:46: 24000000 INFO @ Sat, 15 Jan 2022 19:10:53: 21000000 INFO @ Sat, 15 Jan 2022 19:10:56: 28000000 INFO @ Sat, 15 Jan 2022 19:10:57: 25000000 INFO @ Sat, 15 Jan 2022 19:11:04: 22000000 INFO @ Sat, 15 Jan 2022 19:11:07: 29000000 INFO @ Sat, 15 Jan 2022 19:11:09: 26000000 INFO @ Sat, 15 Jan 2022 19:11:16: 23000000 INFO @ Sat, 15 Jan 2022 19:11:18: 30000000 INFO @ Sat, 15 Jan 2022 19:11:20: 27000000 INFO @ Sat, 15 Jan 2022 19:11:27: 24000000 INFO @ Sat, 15 Jan 2022 19:11:30: 31000000 INFO @ Sat, 15 Jan 2022 19:11:31: 28000000 INFO @ Sat, 15 Jan 2022 19:11:39: 25000000 INFO @ Sat, 15 Jan 2022 19:11:41: 32000000 INFO @ Sat, 15 Jan 2022 19:11:42: 29000000 INFO @ Sat, 15 Jan 2022 19:11:50: 26000000 INFO @ Sat, 15 Jan 2022 19:11:52: 33000000 INFO @ Sat, 15 Jan 2022 19:11:53: 30000000 INFO @ Sat, 15 Jan 2022 19:11:57: #1 tag size is determined as 119 bps INFO @ Sat, 15 Jan 2022 19:11:57: #1 tag size = 119 INFO @ Sat, 15 Jan 2022 19:11:57: #1 total tags in treatment: 16200049 INFO @ Sat, 15 Jan 2022 19:11:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:11:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:11:58: #1 tags after filtering in treatment: 10081002 INFO @ Sat, 15 Jan 2022 19:11:58: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:11:58: #1 finished! INFO @ Sat, 15 Jan 2022 19:11:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:11:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:11:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:11:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:11:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:12:03: 27000000 INFO @ Sat, 15 Jan 2022 19:12:04: 31000000 INFO @ Sat, 15 Jan 2022 19:12:14: 32000000 INFO @ Sat, 15 Jan 2022 19:12:15: 28000000 INFO @ Sat, 15 Jan 2022 19:12:25: 33000000 INFO @ Sat, 15 Jan 2022 19:12:27: 29000000 INFO @ Sat, 15 Jan 2022 19:12:31: #1 tag size is determined as 119 bps INFO @ Sat, 15 Jan 2022 19:12:31: #1 tag size = 119 INFO @ Sat, 15 Jan 2022 19:12:31: #1 total tags in treatment: 16200049 INFO @ Sat, 15 Jan 2022 19:12:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:12:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:12:31: #1 tags after filtering in treatment: 10081002 INFO @ Sat, 15 Jan 2022 19:12:31: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:12:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:12:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:12:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:12:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:12:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:12:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:12:39: 30000000 INFO @ Sat, 15 Jan 2022 19:12:50: 31000000 INFO @ Sat, 15 Jan 2022 19:13:00: 32000000 INFO @ Sat, 15 Jan 2022 19:13:11: 33000000 INFO @ Sat, 15 Jan 2022 19:13:16: #1 tag size is determined as 119 bps INFO @ Sat, 15 Jan 2022 19:13:16: #1 tag size = 119 INFO @ Sat, 15 Jan 2022 19:13:16: #1 total tags in treatment: 16200049 INFO @ Sat, 15 Jan 2022 19:13:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:13:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:13:17: #1 tags after filtering in treatment: 10081002 INFO @ Sat, 15 Jan 2022 19:13:17: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 19:13:17: #1 finished! INFO @ Sat, 15 Jan 2022 19:13:17: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:13:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:13:17: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:13:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:13:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345661/SRX9345661.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling