Job ID = 14520143 SRX = SRX9345660 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21320159 spots for SRR12879684/SRR12879684.sra Written 21320159 spots for SRR12879684/SRR12879684.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:50 21320159 reads; of these: 21320159 (100.00%) were paired; of these: 2029972 (9.52%) aligned concordantly 0 times 15650532 (73.41%) aligned concordantly exactly 1 time 3639655 (17.07%) aligned concordantly >1 times ---- 2029972 pairs aligned concordantly 0 times; of these: 345344 (17.01%) aligned discordantly 1 time ---- 1684628 pairs aligned 0 times concordantly or discordantly; of these: 3369256 mates make up the pairs; of these: 2380562 (70.66%) aligned 0 times 666378 (19.78%) aligned exactly 1 time 322316 (9.57%) aligned >1 times 94.42% overall alignment rate Time searching: 00:31:50 Overall time: 00:31:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 318019 / 6992555 = 0.0455 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:21:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:21:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:21:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:21:56: 1000000 INFO @ Sat, 15 Jan 2022 19:22:04: 2000000 INFO @ Sat, 15 Jan 2022 19:22:12: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:22:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:22:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:22:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:22:19: 4000000 INFO @ Sat, 15 Jan 2022 19:22:28: 5000000 INFO @ Sat, 15 Jan 2022 19:22:28: 1000000 INFO @ Sat, 15 Jan 2022 19:22:36: 6000000 INFO @ Sat, 15 Jan 2022 19:22:37: 2000000 INFO @ Sat, 15 Jan 2022 19:22:44: 7000000 INFO @ Sat, 15 Jan 2022 19:22:45: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:22:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:22:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:22:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:22:52: 8000000 INFO @ Sat, 15 Jan 2022 19:22:55: 4000000 INFO @ Sat, 15 Jan 2022 19:22:58: 1000000 INFO @ Sat, 15 Jan 2022 19:23:01: 9000000 INFO @ Sat, 15 Jan 2022 19:23:04: 5000000 INFO @ Sat, 15 Jan 2022 19:23:07: 2000000 INFO @ Sat, 15 Jan 2022 19:23:10: 10000000 INFO @ Sat, 15 Jan 2022 19:23:13: 6000000 INFO @ Sat, 15 Jan 2022 19:23:17: 3000000 INFO @ Sat, 15 Jan 2022 19:23:19: 11000000 INFO @ Sat, 15 Jan 2022 19:23:21: 7000000 INFO @ Sat, 15 Jan 2022 19:23:26: 4000000 INFO @ Sat, 15 Jan 2022 19:23:29: 12000000 INFO @ Sat, 15 Jan 2022 19:23:30: 8000000 INFO @ Sat, 15 Jan 2022 19:23:35: 5000000 INFO @ Sat, 15 Jan 2022 19:23:38: 13000000 INFO @ Sat, 15 Jan 2022 19:23:39: 9000000 INFO @ Sat, 15 Jan 2022 19:23:44: 6000000 INFO @ Sat, 15 Jan 2022 19:23:47: 14000000 INFO @ Sat, 15 Jan 2022 19:23:47: 10000000 INFO @ Sat, 15 Jan 2022 19:23:53: 7000000 INFO @ Sat, 15 Jan 2022 19:23:56: 15000000 INFO @ Sat, 15 Jan 2022 19:23:56: 11000000 INFO @ Sat, 15 Jan 2022 19:24:02: 8000000 INFO @ Sat, 15 Jan 2022 19:24:05: 16000000 INFO @ Sat, 15 Jan 2022 19:24:05: 12000000 INFO @ Sat, 15 Jan 2022 19:24:11: 9000000 INFO @ Sat, 15 Jan 2022 19:24:14: 17000000 INFO @ Sat, 15 Jan 2022 19:24:15: 13000000 INFO @ Sat, 15 Jan 2022 19:24:20: 10000000 INFO @ Sat, 15 Jan 2022 19:24:22: 18000000 INFO @ Sat, 15 Jan 2022 19:24:25: 14000000 INFO @ Sat, 15 Jan 2022 19:24:29: 11000000 INFO @ Sat, 15 Jan 2022 19:24:32: 19000000 INFO @ Sat, 15 Jan 2022 19:24:34: 15000000 INFO @ Sat, 15 Jan 2022 19:24:38: 12000000 INFO @ Sat, 15 Jan 2022 19:24:41: 20000000 INFO @ Sat, 15 Jan 2022 19:24:43: 16000000 INFO @ Sat, 15 Jan 2022 19:24:47: 13000000 INFO @ Sat, 15 Jan 2022 19:24:50: 21000000 INFO @ Sat, 15 Jan 2022 19:24:52: 17000000 INFO @ Sat, 15 Jan 2022 19:24:57: 14000000 INFO @ Sat, 15 Jan 2022 19:24:59: 22000000 INFO @ Sat, 15 Jan 2022 19:25:02: 18000000 INFO @ Sat, 15 Jan 2022 19:25:06: 15000000 INFO @ Sat, 15 Jan 2022 19:25:09: 23000000 INFO @ Sat, 15 Jan 2022 19:25:12: 19000000 INFO @ Sat, 15 Jan 2022 19:25:15: 16000000 INFO @ Sat, 15 Jan 2022 19:25:19: 24000000 INFO @ Sat, 15 Jan 2022 19:25:22: 20000000 INFO @ Sat, 15 Jan 2022 19:25:24: 17000000 INFO @ Sat, 15 Jan 2022 19:25:28: 25000000 INFO @ Sat, 15 Jan 2022 19:25:31: 21000000 INFO @ Sat, 15 Jan 2022 19:25:33: 18000000 INFO @ Sat, 15 Jan 2022 19:25:38: 26000000 INFO @ Sat, 15 Jan 2022 19:25:40: 22000000 INFO @ Sat, 15 Jan 2022 19:25:42: 19000000 INFO @ Sat, 15 Jan 2022 19:25:47: 27000000 INFO @ Sat, 15 Jan 2022 19:25:50: 23000000 INFO @ Sat, 15 Jan 2022 19:25:52: 20000000 INFO @ Sat, 15 Jan 2022 19:25:56: 28000000 INFO @ Sat, 15 Jan 2022 19:25:59: 24000000 INFO @ Sat, 15 Jan 2022 19:26:01: 21000000 INFO @ Sat, 15 Jan 2022 19:26:05: 29000000 INFO @ Sat, 15 Jan 2022 19:26:08: 25000000 INFO @ Sat, 15 Jan 2022 19:26:10: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:26:14: 30000000 INFO @ Sat, 15 Jan 2022 19:26:17: 26000000 INFO @ Sat, 15 Jan 2022 19:26:19: 23000000 INFO @ Sat, 15 Jan 2022 19:26:23: 31000000 INFO @ Sat, 15 Jan 2022 19:26:27: 27000000 INFO @ Sat, 15 Jan 2022 19:26:28: 24000000 INFO @ Sat, 15 Jan 2022 19:26:32: 32000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:26:36: 28000000 INFO @ Sat, 15 Jan 2022 19:26:38: 25000000 INFO @ Sat, 15 Jan 2022 19:26:41: 33000000 INFO @ Sat, 15 Jan 2022 19:26:46: 29000000 INFO @ Sat, 15 Jan 2022 19:26:47: 26000000 INFO @ Sat, 15 Jan 2022 19:26:50: 34000000 INFO @ Sat, 15 Jan 2022 19:26:56: 27000000 INFO @ Sat, 15 Jan 2022 19:26:56: 30000000 INFO @ Sat, 15 Jan 2022 19:26:59: 35000000 INFO @ Sat, 15 Jan 2022 19:27:05: 28000000 INFO @ Sat, 15 Jan 2022 19:27:07: 31000000 INFO @ Sat, 15 Jan 2022 19:27:08: 36000000 INFO @ Sat, 15 Jan 2022 19:27:14: 29000000 INFO @ Sat, 15 Jan 2022 19:27:16: 32000000 INFO @ Sat, 15 Jan 2022 19:27:17: 37000000 INFO @ Sat, 15 Jan 2022 19:27:23: 30000000 INFO @ Sat, 15 Jan 2022 19:27:26: 38000000 INFO @ Sat, 15 Jan 2022 19:27:26: 33000000 INFO @ Sat, 15 Jan 2022 19:27:32: 31000000 INFO @ Sat, 15 Jan 2022 19:27:35: 39000000 INFO @ Sat, 15 Jan 2022 19:27:36: 34000000 INFO @ Sat, 15 Jan 2022 19:27:41: #1 tag size is determined as 109 bps INFO @ Sat, 15 Jan 2022 19:27:41: #1 tag size = 109 INFO @ Sat, 15 Jan 2022 19:27:41: #1 total tags in treatment: 18975183 INFO @ Sat, 15 Jan 2022 19:27:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:27:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:27:41: 32000000 INFO @ Sat, 15 Jan 2022 19:27:42: #1 tags after filtering in treatment: 11490111 INFO @ Sat, 15 Jan 2022 19:27:42: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:27:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:27:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:27:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:27:42: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:27:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:27:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:27:46: 35000000 INFO @ Sat, 15 Jan 2022 19:27:49: 33000000 INFO @ Sat, 15 Jan 2022 19:27:55: 36000000 INFO @ Sat, 15 Jan 2022 19:27:57: 34000000 INFO @ Sat, 15 Jan 2022 19:28:05: 37000000 INFO @ Sat, 15 Jan 2022 19:28:05: 35000000 INFO @ Sat, 15 Jan 2022 19:28:13: 36000000 INFO @ Sat, 15 Jan 2022 19:28:15: 38000000 INFO @ Sat, 15 Jan 2022 19:28:21: 37000000 INFO @ Sat, 15 Jan 2022 19:28:25: 39000000 INFO @ Sat, 15 Jan 2022 19:28:29: 38000000 INFO @ Sat, 15 Jan 2022 19:28:31: #1 tag size is determined as 109 bps INFO @ Sat, 15 Jan 2022 19:28:31: #1 tag size = 109 INFO @ Sat, 15 Jan 2022 19:28:31: #1 total tags in treatment: 18975183 INFO @ Sat, 15 Jan 2022 19:28:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:28:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:28:31: #1 tags after filtering in treatment: 11490111 INFO @ Sat, 15 Jan 2022 19:28:31: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:28:31: #1 finished! INFO @ Sat, 15 Jan 2022 19:28:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:28:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:28:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:28:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:28:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:28:37: 39000000 INFO @ Sat, 15 Jan 2022 19:28:42: #1 tag size is determined as 109 bps INFO @ Sat, 15 Jan 2022 19:28:42: #1 tag size = 109 INFO @ Sat, 15 Jan 2022 19:28:42: #1 total tags in treatment: 18975183 INFO @ Sat, 15 Jan 2022 19:28:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:28:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:28:42: #1 tags after filtering in treatment: 11490111 INFO @ Sat, 15 Jan 2022 19:28:42: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:28:42: #1 finished! INFO @ Sat, 15 Jan 2022 19:28:42: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:28:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:28:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:28:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:28:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345660/SRX9345660.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling