Job ID = 14520141 SRX = SRX9345658 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21423322 spots for SRR12879682/SRR12879682.sra Written 21423322 spots for SRR12879682/SRR12879682.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:51 21423322 reads; of these: 21423322 (100.00%) were paired; of these: 2312665 (10.80%) aligned concordantly 0 times 15331478 (71.56%) aligned concordantly exactly 1 time 3779179 (17.64%) aligned concordantly >1 times ---- 2312665 pairs aligned concordantly 0 times; of these: 246442 (10.66%) aligned discordantly 1 time ---- 2066223 pairs aligned 0 times concordantly or discordantly; of these: 4132446 mates make up the pairs; of these: 3269788 (79.12%) aligned 0 times 584815 (14.15%) aligned exactly 1 time 277843 (6.72%) aligned >1 times 92.37% overall alignment rate Time searching: 00:31:51 Overall time: 00:31:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 372031 / 7635125 = 0.0487 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:22:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:22:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:22:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:22:09: 1000000 INFO @ Sat, 15 Jan 2022 19:22:18: 2000000 INFO @ Sat, 15 Jan 2022 19:22:26: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:22:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:22:30: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:22:30: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:22:35: 4000000 INFO @ Sat, 15 Jan 2022 19:22:41: 1000000 INFO @ Sat, 15 Jan 2022 19:22:44: 5000000 INFO @ Sat, 15 Jan 2022 19:22:52: 2000000 INFO @ Sat, 15 Jan 2022 19:22:53: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:23:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:23:00: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:23:00: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:23:02: 7000000 INFO @ Sat, 15 Jan 2022 19:23:03: 3000000 INFO @ Sat, 15 Jan 2022 19:23:11: 1000000 INFO @ Sat, 15 Jan 2022 19:23:12: 8000000 INFO @ Sat, 15 Jan 2022 19:23:14: 4000000 INFO @ Sat, 15 Jan 2022 19:23:21: 9000000 INFO @ Sat, 15 Jan 2022 19:23:23: 2000000 INFO @ Sat, 15 Jan 2022 19:23:25: 5000000 INFO @ Sat, 15 Jan 2022 19:23:31: 10000000 INFO @ Sat, 15 Jan 2022 19:23:34: 3000000 INFO @ Sat, 15 Jan 2022 19:23:37: 6000000 INFO @ Sat, 15 Jan 2022 19:23:41: 11000000 INFO @ Sat, 15 Jan 2022 19:23:46: 4000000 INFO @ Sat, 15 Jan 2022 19:23:49: 7000000 INFO @ Sat, 15 Jan 2022 19:23:50: 12000000 INFO @ Sat, 15 Jan 2022 19:23:57: 5000000 INFO @ Sat, 15 Jan 2022 19:24:00: 13000000 INFO @ Sat, 15 Jan 2022 19:24:00: 8000000 INFO @ Sat, 15 Jan 2022 19:24:08: 6000000 INFO @ Sat, 15 Jan 2022 19:24:10: 14000000 INFO @ Sat, 15 Jan 2022 19:24:13: 9000000 INFO @ Sat, 15 Jan 2022 19:24:19: 7000000 INFO @ Sat, 15 Jan 2022 19:24:20: 15000000 INFO @ Sat, 15 Jan 2022 19:24:25: 10000000 INFO @ Sat, 15 Jan 2022 19:24:29: 16000000 INFO @ Sat, 15 Jan 2022 19:24:30: 8000000 INFO @ Sat, 15 Jan 2022 19:24:38: 11000000 INFO @ Sat, 15 Jan 2022 19:24:39: 17000000 INFO @ Sat, 15 Jan 2022 19:24:43: 9000000 INFO @ Sat, 15 Jan 2022 19:24:49: 18000000 INFO @ Sat, 15 Jan 2022 19:24:50: 12000000 INFO @ Sat, 15 Jan 2022 19:24:54: 10000000 INFO @ Sat, 15 Jan 2022 19:24:58: 19000000 INFO @ Sat, 15 Jan 2022 19:25:01: 13000000 INFO @ Sat, 15 Jan 2022 19:25:05: 11000000 INFO @ Sat, 15 Jan 2022 19:25:07: 20000000 INFO @ Sat, 15 Jan 2022 19:25:13: 14000000 INFO @ Sat, 15 Jan 2022 19:25:16: 21000000 INFO @ Sat, 15 Jan 2022 19:25:17: 12000000 INFO @ Sat, 15 Jan 2022 19:25:24: 15000000 INFO @ Sat, 15 Jan 2022 19:25:27: 22000000 INFO @ Sat, 15 Jan 2022 19:25:28: 13000000 INFO @ Sat, 15 Jan 2022 19:25:35: 16000000 INFO @ Sat, 15 Jan 2022 19:25:36: 23000000 INFO @ Sat, 15 Jan 2022 19:25:39: 14000000 INFO @ Sat, 15 Jan 2022 19:25:46: 24000000 INFO @ Sat, 15 Jan 2022 19:25:47: 17000000 INFO @ Sat, 15 Jan 2022 19:25:51: 15000000 INFO @ Sat, 15 Jan 2022 19:25:56: 25000000 INFO @ Sat, 15 Jan 2022 19:25:58: 18000000 INFO @ Sat, 15 Jan 2022 19:26:02: 16000000 INFO @ Sat, 15 Jan 2022 19:26:05: 26000000 INFO @ Sat, 15 Jan 2022 19:26:09: 19000000 INFO @ Sat, 15 Jan 2022 19:26:14: 17000000 INFO @ Sat, 15 Jan 2022 19:26:15: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:26:21: 20000000 INFO @ Sat, 15 Jan 2022 19:26:24: 28000000 INFO @ Sat, 15 Jan 2022 19:26:25: 18000000 INFO @ Sat, 15 Jan 2022 19:26:32: 21000000 INFO @ Sat, 15 Jan 2022 19:26:34: 29000000 INFO @ Sat, 15 Jan 2022 19:26:37: 19000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:26:43: 30000000 INFO @ Sat, 15 Jan 2022 19:26:43: 22000000 INFO @ Sat, 15 Jan 2022 19:26:49: 20000000 INFO @ Sat, 15 Jan 2022 19:26:53: 31000000 INFO @ Sat, 15 Jan 2022 19:26:55: 23000000 INFO @ Sat, 15 Jan 2022 19:27:00: 21000000 INFO @ Sat, 15 Jan 2022 19:27:02: 32000000 INFO @ Sat, 15 Jan 2022 19:27:08: 24000000 INFO @ Sat, 15 Jan 2022 19:27:11: 22000000 INFO @ Sat, 15 Jan 2022 19:27:12: 33000000 INFO @ Sat, 15 Jan 2022 19:27:21: 25000000 INFO @ Sat, 15 Jan 2022 19:27:22: 34000000 INFO @ Sat, 15 Jan 2022 19:27:23: 23000000 INFO @ Sat, 15 Jan 2022 19:27:31: 35000000 INFO @ Sat, 15 Jan 2022 19:27:33: 26000000 INFO @ Sat, 15 Jan 2022 19:27:34: 24000000 INFO @ Sat, 15 Jan 2022 19:27:40: 36000000 INFO @ Sat, 15 Jan 2022 19:27:44: 27000000 INFO @ Sat, 15 Jan 2022 19:27:46: 25000000 INFO @ Sat, 15 Jan 2022 19:27:50: 37000000 INFO @ Sat, 15 Jan 2022 19:27:56: 28000000 INFO @ Sat, 15 Jan 2022 19:27:58: 26000000 INFO @ Sat, 15 Jan 2022 19:27:59: 38000000 INFO @ Sat, 15 Jan 2022 19:28:07: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 19:28:07: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 19:28:07: #1 total tags in treatment: 18740316 INFO @ Sat, 15 Jan 2022 19:28:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:28:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:28:07: 29000000 INFO @ Sat, 15 Jan 2022 19:28:08: #1 tags after filtering in treatment: 11342031 INFO @ Sat, 15 Jan 2022 19:28:08: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:28:08: #1 finished! INFO @ Sat, 15 Jan 2022 19:28:08: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:28:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:28:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:28:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:28:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 207 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:28:09: 27000000 INFO @ Sat, 15 Jan 2022 19:28:19: 30000000 INFO @ Sat, 15 Jan 2022 19:28:21: 28000000 INFO @ Sat, 15 Jan 2022 19:28:30: 31000000 INFO @ Sat, 15 Jan 2022 19:28:32: 29000000 INFO @ Sat, 15 Jan 2022 19:28:42: 32000000 INFO @ Sat, 15 Jan 2022 19:28:43: 30000000 INFO @ Sat, 15 Jan 2022 19:28:53: 33000000 INFO @ Sat, 15 Jan 2022 19:28:55: 31000000 INFO @ Sat, 15 Jan 2022 19:29:04: 34000000 INFO @ Sat, 15 Jan 2022 19:29:06: 32000000 INFO @ Sat, 15 Jan 2022 19:29:16: 35000000 INFO @ Sat, 15 Jan 2022 19:29:17: 33000000 INFO @ Sat, 15 Jan 2022 19:29:27: 36000000 INFO @ Sat, 15 Jan 2022 19:29:29: 34000000 INFO @ Sat, 15 Jan 2022 19:29:38: 37000000 INFO @ Sat, 15 Jan 2022 19:29:40: 35000000 INFO @ Sat, 15 Jan 2022 19:29:50: 38000000 INFO @ Sat, 15 Jan 2022 19:29:51: 36000000 INFO @ Sat, 15 Jan 2022 19:30:00: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 19:30:00: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 19:30:00: #1 total tags in treatment: 18740316 INFO @ Sat, 15 Jan 2022 19:30:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:01: #1 tags after filtering in treatment: 11342031 INFO @ Sat, 15 Jan 2022 19:30:01: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:30:01: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:30:02: 37000000 INFO @ Sat, 15 Jan 2022 19:30:13: 38000000 INFO @ Sat, 15 Jan 2022 19:30:22: #1 tag size is determined as 96 bps INFO @ Sat, 15 Jan 2022 19:30:22: #1 tag size = 96 INFO @ Sat, 15 Jan 2022 19:30:22: #1 total tags in treatment: 18740316 INFO @ Sat, 15 Jan 2022 19:30:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:23: #1 tags after filtering in treatment: 11342031 INFO @ Sat, 15 Jan 2022 19:30:23: #1 Redundant rate of treatment: 0.39 INFO @ Sat, 15 Jan 2022 19:30:23: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9345658/SRX9345658.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling