Job ID = 14521332 SRX = SRX9278498 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 2495187 spots for SRR12809896/SRR12809896.sra Written 2495187 spots for SRR12809896/SRR12809896.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:13 2495187 reads; of these: 2495187 (100.00%) were unpaired; of these: 69079 (2.77%) aligned 0 times 2119233 (84.93%) aligned exactly 1 time 306875 (12.30%) aligned >1 times 97.23% overall alignment rate Time searching: 00:01:13 Overall time: 00:01:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 318221 / 2426108 = 0.1312 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:53:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:53:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:53:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:53:20: 1000000 INFO @ Sat, 15 Jan 2022 20:53:29: 2000000 INFO @ Sat, 15 Jan 2022 20:53:30: #1 tag size is determined as 142 bps INFO @ Sat, 15 Jan 2022 20:53:30: #1 tag size = 142 INFO @ Sat, 15 Jan 2022 20:53:30: #1 total tags in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:53:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:53:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:53:30: #1 tags after filtering in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:53:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:53:30: #1 finished! INFO @ Sat, 15 Jan 2022 20:53:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:53:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:53:30: #2 number of paired peaks: 52 WARNING @ Sat, 15 Jan 2022 20:53:30: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:53:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:53:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:53:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:53:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:53:48: 1000000 INFO @ Sat, 15 Jan 2022 20:53:56: 2000000 INFO @ Sat, 15 Jan 2022 20:53:56: #1 tag size is determined as 142 bps INFO @ Sat, 15 Jan 2022 20:53:56: #1 tag size = 142 INFO @ Sat, 15 Jan 2022 20:53:56: #1 total tags in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:53:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:53:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:53:56: #1 tags after filtering in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:53:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:53:56: #1 finished! INFO @ Sat, 15 Jan 2022 20:53:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:53:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:53:57: #2 number of paired peaks: 52 WARNING @ Sat, 15 Jan 2022 20:53:57: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:53:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:54:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:54:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:54:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:54:19: 1000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:54:27: 2000000 INFO @ Sat, 15 Jan 2022 20:54:28: #1 tag size is determined as 142 bps INFO @ Sat, 15 Jan 2022 20:54:28: #1 tag size = 142 INFO @ Sat, 15 Jan 2022 20:54:28: #1 total tags in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:54:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:54:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:54:28: #1 tags after filtering in treatment: 2107887 INFO @ Sat, 15 Jan 2022 20:54:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 20:54:28: #1 finished! INFO @ Sat, 15 Jan 2022 20:54:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:54:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:54:28: #2 number of paired peaks: 52 WARNING @ Sat, 15 Jan 2022 20:54:28: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:54:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9278498/SRX9278498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling