Job ID = 14520996 SRX = SRX9158104 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9878075 spots for SRR12677939/SRR12677939.sra Written 9878075 spots for SRR12677939/SRR12677939.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 9878075 reads; of these: 9878075 (100.00%) were paired; of these: 344081 (3.48%) aligned concordantly 0 times 8532652 (86.38%) aligned concordantly exactly 1 time 1001342 (10.14%) aligned concordantly >1 times ---- 344081 pairs aligned concordantly 0 times; of these: 5020 (1.46%) aligned discordantly 1 time ---- 339061 pairs aligned 0 times concordantly or discordantly; of these: 678122 mates make up the pairs; of these: 588797 (86.83%) aligned 0 times 71688 (10.57%) aligned exactly 1 time 17637 (2.60%) aligned >1 times 97.02% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2402954 / 9538043 = 0.2519 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:26:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:26:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:26:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:00: 1000000 INFO @ Sat, 15 Jan 2022 20:27:06: 2000000 INFO @ Sat, 15 Jan 2022 20:27:11: 3000000 INFO @ Sat, 15 Jan 2022 20:27:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:23: 5000000 INFO @ Sat, 15 Jan 2022 20:27:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:29: 6000000 INFO @ Sat, 15 Jan 2022 20:27:31: 1000000 INFO @ Sat, 15 Jan 2022 20:27:36: 7000000 INFO @ Sat, 15 Jan 2022 20:27:36: 2000000 INFO @ Sat, 15 Jan 2022 20:27:42: 3000000 INFO @ Sat, 15 Jan 2022 20:27:42: 8000000 INFO @ Sat, 15 Jan 2022 20:27:48: 4000000 INFO @ Sat, 15 Jan 2022 20:27:49: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:53: 5000000 INFO @ Sat, 15 Jan 2022 20:27:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:54: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:54: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:27:56: 10000000 INFO @ Sat, 15 Jan 2022 20:27:59: 6000000 INFO @ Sat, 15 Jan 2022 20:28:00: 1000000 INFO @ Sat, 15 Jan 2022 20:28:02: 11000000 INFO @ Sat, 15 Jan 2022 20:28:05: 7000000 INFO @ Sat, 15 Jan 2022 20:28:06: 2000000 INFO @ Sat, 15 Jan 2022 20:28:08: 12000000 INFO @ Sat, 15 Jan 2022 20:28:11: 8000000 INFO @ Sat, 15 Jan 2022 20:28:12: 3000000 INFO @ Sat, 15 Jan 2022 20:28:15: 13000000 INFO @ Sat, 15 Jan 2022 20:28:17: 9000000 INFO @ Sat, 15 Jan 2022 20:28:18: 4000000 INFO @ Sat, 15 Jan 2022 20:28:21: 14000000 INFO @ Sat, 15 Jan 2022 20:28:22: 10000000 INFO @ Sat, 15 Jan 2022 20:28:23: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:28:23: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:28:23: #1 total tags in treatment: 7132186 INFO @ Sat, 15 Jan 2022 20:28:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:28:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:28:24: #1 tags after filtering in treatment: 5520385 INFO @ Sat, 15 Jan 2022 20:28:24: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:28:24: #1 finished! INFO @ Sat, 15 Jan 2022 20:28:24: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:28:24: 5000000 INFO @ Sat, 15 Jan 2022 20:28:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:28:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:28:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:28:28: 11000000 INFO @ Sat, 15 Jan 2022 20:28:29: 6000000 INFO @ Sat, 15 Jan 2022 20:28:33: 12000000 INFO @ Sat, 15 Jan 2022 20:28:34: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:28:38: 13000000 INFO @ Sat, 15 Jan 2022 20:28:40: 8000000 INFO @ Sat, 15 Jan 2022 20:28:44: 14000000 INFO @ Sat, 15 Jan 2022 20:28:45: 9000000 INFO @ Sat, 15 Jan 2022 20:28:45: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:28:45: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:28:45: #1 total tags in treatment: 7132186 INFO @ Sat, 15 Jan 2022 20:28:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:28:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:28:45: #1 tags after filtering in treatment: 5520385 INFO @ Sat, 15 Jan 2022 20:28:45: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:28:45: #1 finished! INFO @ Sat, 15 Jan 2022 20:28:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:28:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:28:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:28:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:28:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:28:50: 10000000 INFO @ Sat, 15 Jan 2022 20:28:55: 11000000 INFO @ Sat, 15 Jan 2022 20:29:00: 12000000 INFO @ Sat, 15 Jan 2022 20:29:05: 13000000 INFO @ Sat, 15 Jan 2022 20:29:10: 14000000 INFO @ Sat, 15 Jan 2022 20:29:11: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:29:11: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:29:11: #1 total tags in treatment: 7132186 INFO @ Sat, 15 Jan 2022 20:29:11: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:11: #1 tags after filtering in treatment: 5520385 INFO @ Sat, 15 Jan 2022 20:29:11: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 20:29:11: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:11: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:12: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158104/SRX9158104.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling