Job ID = 14520994 SRX = SRX9158102 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10504739 spots for SRR12677937/SRR12677937.sra Written 10504739 spots for SRR12677937/SRR12677937.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 10504739 reads; of these: 10504739 (100.00%) were paired; of these: 669461 (6.37%) aligned concordantly 0 times 8683729 (82.66%) aligned concordantly exactly 1 time 1151549 (10.96%) aligned concordantly >1 times ---- 669461 pairs aligned concordantly 0 times; of these: 6016 (0.90%) aligned discordantly 1 time ---- 663445 pairs aligned 0 times concordantly or discordantly; of these: 1326890 mates make up the pairs; of these: 1233047 (92.93%) aligned 0 times 74833 (5.64%) aligned exactly 1 time 19010 (1.43%) aligned >1 times 94.13% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1906225 / 9840054 = 0.1937 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:27:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:27:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:27:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:00: 1000000 INFO @ Sat, 15 Jan 2022 20:28:06: 2000000 INFO @ Sat, 15 Jan 2022 20:28:12: 3000000 INFO @ Sat, 15 Jan 2022 20:28:18: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:23: 5000000 INFO @ Sat, 15 Jan 2022 20:28:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:25: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:25: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:30: 6000000 INFO @ Sat, 15 Jan 2022 20:28:31: 1000000 INFO @ Sat, 15 Jan 2022 20:28:36: 7000000 INFO @ Sat, 15 Jan 2022 20:28:37: 2000000 INFO @ Sat, 15 Jan 2022 20:28:42: 8000000 INFO @ Sat, 15 Jan 2022 20:28:43: 3000000 INFO @ Sat, 15 Jan 2022 20:28:48: 4000000 INFO @ Sat, 15 Jan 2022 20:28:49: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:28:54: 5000000 INFO @ Sat, 15 Jan 2022 20:28:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:28:55: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:28:55: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:28:55: 10000000 INFO @ Sat, 15 Jan 2022 20:29:00: 6000000 INFO @ Sat, 15 Jan 2022 20:29:01: 1000000 INFO @ Sat, 15 Jan 2022 20:29:02: 11000000 INFO @ Sat, 15 Jan 2022 20:29:06: 7000000 INFO @ Sat, 15 Jan 2022 20:29:07: 2000000 INFO @ Sat, 15 Jan 2022 20:29:08: 12000000 INFO @ Sat, 15 Jan 2022 20:29:11: 8000000 INFO @ Sat, 15 Jan 2022 20:29:14: 3000000 INFO @ Sat, 15 Jan 2022 20:29:15: 13000000 INFO @ Sat, 15 Jan 2022 20:29:17: 9000000 INFO @ Sat, 15 Jan 2022 20:29:21: 4000000 INFO @ Sat, 15 Jan 2022 20:29:21: 14000000 INFO @ Sat, 15 Jan 2022 20:29:23: 10000000 INFO @ Sat, 15 Jan 2022 20:29:27: 5000000 INFO @ Sat, 15 Jan 2022 20:29:28: 15000000 INFO @ Sat, 15 Jan 2022 20:29:29: 11000000 INFO @ Sat, 15 Jan 2022 20:29:34: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:29:34: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:29:34: #1 total tags in treatment: 7929906 INFO @ Sat, 15 Jan 2022 20:29:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:29:34: #1 tags after filtering in treatment: 5917126 INFO @ Sat, 15 Jan 2022 20:29:34: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:29:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:34: 6000000 INFO @ Sat, 15 Jan 2022 20:29:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:29:35: 12000000 INFO @ Sat, 15 Jan 2022 20:29:40: 13000000 INFO @ Sat, 15 Jan 2022 20:29:41: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:29:46: 14000000 INFO @ Sat, 15 Jan 2022 20:29:48: 8000000 INFO @ Sat, 15 Jan 2022 20:29:52: 15000000 INFO @ Sat, 15 Jan 2022 20:29:54: 9000000 INFO @ Sat, 15 Jan 2022 20:29:57: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:29:57: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:29:57: #1 total tags in treatment: 7929906 INFO @ Sat, 15 Jan 2022 20:29:57: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:29:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:29:57: #1 tags after filtering in treatment: 5917126 INFO @ Sat, 15 Jan 2022 20:29:57: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:29:57: #1 finished! INFO @ Sat, 15 Jan 2022 20:29:57: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:29:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:29:57: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:29:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:29:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:30:01: 10000000 INFO @ Sat, 15 Jan 2022 20:30:07: 11000000 INFO @ Sat, 15 Jan 2022 20:30:12: 12000000 INFO @ Sat, 15 Jan 2022 20:30:18: 13000000 INFO @ Sat, 15 Jan 2022 20:30:24: 14000000 INFO @ Sat, 15 Jan 2022 20:30:30: 15000000 INFO @ Sat, 15 Jan 2022 20:30:35: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 20:30:35: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 20:30:35: #1 total tags in treatment: 7929906 INFO @ Sat, 15 Jan 2022 20:30:35: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:30:36: #1 tags after filtering in treatment: 5917126 INFO @ Sat, 15 Jan 2022 20:30:36: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 20:30:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:30:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:30:36: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:30:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:30:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9158102/SRX9158102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling