Job ID = 14520577 SRX = SRX9144942 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19787076 spots for SRR12664300/SRR12664300.sra Written 19787076 spots for SRR12664300/SRR12664300.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:28 19787076 reads; of these: 19787076 (100.00%) were paired; of these: 980532 (4.96%) aligned concordantly 0 times 15769626 (79.70%) aligned concordantly exactly 1 time 3036918 (15.35%) aligned concordantly >1 times ---- 980532 pairs aligned concordantly 0 times; of these: 151464 (15.45%) aligned discordantly 1 time ---- 829068 pairs aligned 0 times concordantly or discordantly; of these: 1658136 mates make up the pairs; of these: 1480817 (89.31%) aligned 0 times 73345 (4.42%) aligned exactly 1 time 103974 (6.27%) aligned >1 times 96.26% overall alignment rate Time searching: 00:38:28 Overall time: 00:38:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 8599213 / 18945462 = 0.4539 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:25: 1000000 INFO @ Sat, 15 Jan 2022 20:22:33: 2000000 INFO @ Sat, 15 Jan 2022 20:22:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:22:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:22:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:22:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:22:51: 4000000 INFO @ Sat, 15 Jan 2022 20:22:59: 1000000 INFO @ Sat, 15 Jan 2022 20:23:00: 5000000 INFO @ Sat, 15 Jan 2022 20:23:09: 6000000 INFO @ Sat, 15 Jan 2022 20:23:11: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:23:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:23:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:23:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:23:18: 7000000 INFO @ Sat, 15 Jan 2022 20:23:22: 3000000 INFO @ Sat, 15 Jan 2022 20:23:27: 1000000 INFO @ Sat, 15 Jan 2022 20:23:29: 8000000 INFO @ Sat, 15 Jan 2022 20:23:34: 4000000 INFO @ Sat, 15 Jan 2022 20:23:37: 2000000 INFO @ Sat, 15 Jan 2022 20:23:39: 9000000 INFO @ Sat, 15 Jan 2022 20:23:46: 5000000 INFO @ Sat, 15 Jan 2022 20:23:47: 3000000 INFO @ Sat, 15 Jan 2022 20:23:49: 10000000 INFO @ Sat, 15 Jan 2022 20:23:57: 4000000 INFO @ Sat, 15 Jan 2022 20:23:59: 6000000 INFO @ Sat, 15 Jan 2022 20:24:01: 11000000 INFO @ Sat, 15 Jan 2022 20:24:08: 5000000 INFO @ Sat, 15 Jan 2022 20:24:11: 7000000 INFO @ Sat, 15 Jan 2022 20:24:13: 12000000 INFO @ Sat, 15 Jan 2022 20:24:18: 6000000 INFO @ Sat, 15 Jan 2022 20:24:23: 13000000 INFO @ Sat, 15 Jan 2022 20:24:23: 8000000 INFO @ Sat, 15 Jan 2022 20:24:28: 7000000 INFO @ Sat, 15 Jan 2022 20:24:33: 14000000 INFO @ Sat, 15 Jan 2022 20:24:36: 9000000 INFO @ Sat, 15 Jan 2022 20:24:38: 8000000 INFO @ Sat, 15 Jan 2022 20:24:44: 15000000 INFO @ Sat, 15 Jan 2022 20:24:48: 9000000 INFO @ Sat, 15 Jan 2022 20:24:49: 10000000 INFO @ Sat, 15 Jan 2022 20:24:54: 16000000 INFO @ Sat, 15 Jan 2022 20:24:58: 10000000 INFO @ Sat, 15 Jan 2022 20:25:01: 11000000 INFO @ Sat, 15 Jan 2022 20:25:04: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:25:09: 11000000 INFO @ Sat, 15 Jan 2022 20:25:14: 18000000 INFO @ Sat, 15 Jan 2022 20:25:15: 12000000 INFO @ Sat, 15 Jan 2022 20:25:19: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:25:25: 19000000 INFO @ Sat, 15 Jan 2022 20:25:26: 13000000 INFO @ Sat, 15 Jan 2022 20:25:29: 13000000 INFO @ Sat, 15 Jan 2022 20:25:35: 20000000 INFO @ Sat, 15 Jan 2022 20:25:39: 14000000 INFO @ Sat, 15 Jan 2022 20:25:39: 14000000 INFO @ Sat, 15 Jan 2022 20:25:44: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:25:44: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:25:44: #1 total tags in treatment: 10267541 INFO @ Sat, 15 Jan 2022 20:25:44: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:25:44: #1 tags after filtering in treatment: 7347376 INFO @ Sat, 15 Jan 2022 20:25:44: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:25:44: #1 finished! INFO @ Sat, 15 Jan 2022 20:25:44: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:25:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:25:45: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:25:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:25:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:25:49: 15000000 INFO @ Sat, 15 Jan 2022 20:25:51: 15000000 INFO @ Sat, 15 Jan 2022 20:25:58: 16000000 INFO @ Sat, 15 Jan 2022 20:26:03: 16000000 INFO @ Sat, 15 Jan 2022 20:26:07: 17000000 INFO @ Sat, 15 Jan 2022 20:26:15: 17000000 INFO @ Sat, 15 Jan 2022 20:26:17: 18000000 INFO @ Sat, 15 Jan 2022 20:26:26: 19000000 INFO @ Sat, 15 Jan 2022 20:26:26: 18000000 INFO @ Sat, 15 Jan 2022 20:26:35: 20000000 INFO @ Sat, 15 Jan 2022 20:26:38: 19000000 INFO @ Sat, 15 Jan 2022 20:26:42: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:26:42: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:26:42: #1 total tags in treatment: 10267541 INFO @ Sat, 15 Jan 2022 20:26:42: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:26:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:26:43: #1 tags after filtering in treatment: 7347376 INFO @ Sat, 15 Jan 2022 20:26:43: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:26:43: #1 finished! INFO @ Sat, 15 Jan 2022 20:26:43: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:26:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:26:43: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:26:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:26:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:26:50: 20000000 INFO @ Sat, 15 Jan 2022 20:27:00: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:27:00: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:27:00: #1 total tags in treatment: 10267541 INFO @ Sat, 15 Jan 2022 20:27:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:27:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:27:00: #1 tags after filtering in treatment: 7347376 INFO @ Sat, 15 Jan 2022 20:27:00: #1 Redundant rate of treatment: 0.28 INFO @ Sat, 15 Jan 2022 20:27:00: #1 finished! INFO @ Sat, 15 Jan 2022 20:27:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:27:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:27:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:27:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:27:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144942/SRX9144942.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling