Job ID = 14520576 SRX = SRX9144941 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 15331696 spots for SRR12664299/SRR12664299.sra Written 15331696 spots for SRR12664299/SRR12664299.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:29:36 15331696 reads; of these: 15331696 (100.00%) were paired; of these: 756795 (4.94%) aligned concordantly 0 times 11924658 (77.78%) aligned concordantly exactly 1 time 2650243 (17.29%) aligned concordantly >1 times ---- 756795 pairs aligned concordantly 0 times; of these: 194090 (25.65%) aligned discordantly 1 time ---- 562705 pairs aligned 0 times concordantly or discordantly; of these: 1125410 mates make up the pairs; of these: 889101 (79.00%) aligned 0 times 80787 (7.18%) aligned exactly 1 time 155522 (13.82%) aligned >1 times 97.10% overall alignment rate Time searching: 00:29:36 Overall time: 00:29:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 6525784 / 14765039 = 0.4420 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:16: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:16: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:30: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:14:44: 2000000 INFO @ Sat, 15 Jan 2022 20:14:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:14:46: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:14:46: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:14:57: 3000000 INFO @ Sat, 15 Jan 2022 20:15:00: 1000000 INFO @ Sat, 15 Jan 2022 20:15:10: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:15:15: 2000000 INFO @ Sat, 15 Jan 2022 20:15:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:15:15: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:15:15: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:15:22: 5000000 INFO @ Sat, 15 Jan 2022 20:15:34: 3000000 INFO @ Sat, 15 Jan 2022 20:15:34: 1000000 INFO @ Sat, 15 Jan 2022 20:15:35: 6000000 INFO @ Sat, 15 Jan 2022 20:15:48: 7000000 INFO @ Sat, 15 Jan 2022 20:15:53: 4000000 INFO @ Sat, 15 Jan 2022 20:15:53: 2000000 INFO @ Sat, 15 Jan 2022 20:16:02: 8000000 INFO @ Sat, 15 Jan 2022 20:16:13: 5000000 INFO @ Sat, 15 Jan 2022 20:16:13: 3000000 INFO @ Sat, 15 Jan 2022 20:16:15: 9000000 INFO @ Sat, 15 Jan 2022 20:16:29: 10000000 INFO @ Sat, 15 Jan 2022 20:16:32: 6000000 INFO @ Sat, 15 Jan 2022 20:16:32: 4000000 INFO @ Sat, 15 Jan 2022 20:16:42: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:16:52: 5000000 INFO @ Sat, 15 Jan 2022 20:16:52: 7000000 INFO @ Sat, 15 Jan 2022 20:16:55: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:17:09: 13000000 INFO @ Sat, 15 Jan 2022 20:17:11: 6000000 INFO @ Sat, 15 Jan 2022 20:17:11: 8000000 INFO @ Sat, 15 Jan 2022 20:17:22: 14000000 INFO @ Sat, 15 Jan 2022 20:17:29: 9000000 INFO @ Sat, 15 Jan 2022 20:17:29: 7000000 INFO @ Sat, 15 Jan 2022 20:17:36: 15000000 INFO @ Sat, 15 Jan 2022 20:17:47: 10000000 INFO @ Sat, 15 Jan 2022 20:17:48: 8000000 INFO @ Sat, 15 Jan 2022 20:17:50: 16000000 INFO @ Sat, 15 Jan 2022 20:18:00: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:18:00: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:18:00: #1 total tags in treatment: 8128598 INFO @ Sat, 15 Jan 2022 20:18:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:01: #1 tags after filtering in treatment: 5711510 INFO @ Sat, 15 Jan 2022 20:18:01: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:18:01: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:01: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:18:06: 11000000 INFO @ Sat, 15 Jan 2022 20:18:07: 9000000 INFO @ Sat, 15 Jan 2022 20:18:25: 12000000 INFO @ Sat, 15 Jan 2022 20:18:25: 10000000 INFO @ Sat, 15 Jan 2022 20:18:43: 13000000 INFO @ Sat, 15 Jan 2022 20:18:43: 11000000 INFO @ Sat, 15 Jan 2022 20:19:01: 14000000 INFO @ Sat, 15 Jan 2022 20:19:01: 12000000 INFO @ Sat, 15 Jan 2022 20:19:18: 15000000 INFO @ Sat, 15 Jan 2022 20:19:19: 13000000 INFO @ Sat, 15 Jan 2022 20:19:36: 16000000 INFO @ Sat, 15 Jan 2022 20:19:37: 14000000 INFO @ Sat, 15 Jan 2022 20:19:49: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:19:49: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:19:49: #1 total tags in treatment: 8128598 INFO @ Sat, 15 Jan 2022 20:19:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:49: #1 tags after filtering in treatment: 5711510 INFO @ Sat, 15 Jan 2022 20:19:49: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:19:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:54: 15000000 INFO @ Sat, 15 Jan 2022 20:20:08: 16000000 INFO @ Sat, 15 Jan 2022 20:20:18: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:20:18: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:20:18: #1 total tags in treatment: 8128598 INFO @ Sat, 15 Jan 2022 20:20:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:20:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:20:18: #1 tags after filtering in treatment: 5711510 INFO @ Sat, 15 Jan 2022 20:20:18: #1 Redundant rate of treatment: 0.30 INFO @ Sat, 15 Jan 2022 20:20:18: #1 finished! INFO @ Sat, 15 Jan 2022 20:20:18: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:20:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:20:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:20:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:20:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144941/SRX9144941.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling