Job ID = 14520575 SRX = SRX9144940 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12937586 spots for SRR12664298/SRR12664298.sra Written 12937586 spots for SRR12664298/SRR12664298.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:59 12937586 reads; of these: 12937586 (100.00%) were paired; of these: 388793 (3.01%) aligned concordantly 0 times 9932273 (76.77%) aligned concordantly exactly 1 time 2616520 (20.22%) aligned concordantly >1 times ---- 388793 pairs aligned concordantly 0 times; of these: 42556 (10.95%) aligned discordantly 1 time ---- 346237 pairs aligned 0 times concordantly or discordantly; of these: 692474 mates make up the pairs; of these: 595821 (86.04%) aligned 0 times 44404 (6.41%) aligned exactly 1 time 52249 (7.55%) aligned >1 times 97.70% overall alignment rate Time searching: 00:25:59 Overall time: 00:25:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4706120 / 12587266 = 0.3739 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:03:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:03:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:03:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:03:20: 1000000 INFO @ Sat, 15 Jan 2022 20:03:28: 2000000 INFO @ Sat, 15 Jan 2022 20:03:34: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:03:41: 4000000 INFO @ Sat, 15 Jan 2022 20:03:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:03:43: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:03:43: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:03:49: 5000000 INFO @ Sat, 15 Jan 2022 20:03:52: 1000000 INFO @ Sat, 15 Jan 2022 20:03:57: 6000000 INFO @ Sat, 15 Jan 2022 20:04:00: 2000000 INFO @ Sat, 15 Jan 2022 20:04:05: 7000000 INFO @ Sat, 15 Jan 2022 20:04:08: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:04:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:04:13: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:04:13: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:04:13: 8000000 INFO @ Sat, 15 Jan 2022 20:04:16: 4000000 INFO @ Sat, 15 Jan 2022 20:04:21: 1000000 INFO @ Sat, 15 Jan 2022 20:04:21: 9000000 INFO @ Sat, 15 Jan 2022 20:04:24: 5000000 INFO @ Sat, 15 Jan 2022 20:04:28: 2000000 INFO @ Sat, 15 Jan 2022 20:04:29: 10000000 INFO @ Sat, 15 Jan 2022 20:04:32: 6000000 INFO @ Sat, 15 Jan 2022 20:04:35: 3000000 INFO @ Sat, 15 Jan 2022 20:04:38: 11000000 INFO @ Sat, 15 Jan 2022 20:04:41: 7000000 INFO @ Sat, 15 Jan 2022 20:04:43: 4000000 INFO @ Sat, 15 Jan 2022 20:04:46: 12000000 INFO @ Sat, 15 Jan 2022 20:04:49: 8000000 INFO @ Sat, 15 Jan 2022 20:04:50: 5000000 INFO @ Sat, 15 Jan 2022 20:04:55: 13000000 INFO @ Sat, 15 Jan 2022 20:04:57: 9000000 INFO @ Sat, 15 Jan 2022 20:04:57: 6000000 INFO @ Sat, 15 Jan 2022 20:05:03: 14000000 INFO @ Sat, 15 Jan 2022 20:05:05: 7000000 INFO @ Sat, 15 Jan 2022 20:05:05: 10000000 INFO @ Sat, 15 Jan 2022 20:05:11: 15000000 INFO @ Sat, 15 Jan 2022 20:05:13: 8000000 INFO @ Sat, 15 Jan 2022 20:05:14: 11000000 INFO @ Sat, 15 Jan 2022 20:05:18: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:05:18: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:05:18: #1 total tags in treatment: 7855735 INFO @ Sat, 15 Jan 2022 20:05:18: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:05:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:05:19: #1 tags after filtering in treatment: 5261270 INFO @ Sat, 15 Jan 2022 20:05:19: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:05:19: #1 finished! INFO @ Sat, 15 Jan 2022 20:05:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:05:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:05:19: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:05:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:05:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:05:21: 9000000 INFO @ Sat, 15 Jan 2022 20:05:21: 12000000 INFO @ Sat, 15 Jan 2022 20:05:28: 10000000 INFO @ Sat, 15 Jan 2022 20:05:28: 13000000 INFO @ Sat, 15 Jan 2022 20:05:35: 11000000 INFO @ Sat, 15 Jan 2022 20:05:35: 14000000 INFO @ Sat, 15 Jan 2022 20:05:42: 12000000 INFO @ Sat, 15 Jan 2022 20:05:42: 15000000 INFO @ Sat, 15 Jan 2022 20:05:49: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:05:49: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:05:49: #1 total tags in treatment: 7855735 INFO @ Sat, 15 Jan 2022 20:05:49: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:05:49: #1 tags after filtering in treatment: 5261270 INFO @ Sat, 15 Jan 2022 20:05:49: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:05:49: #1 finished! INFO @ Sat, 15 Jan 2022 20:05:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:05:49: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:05:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:05:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:05:50: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:05:57: 14000000 INFO @ Sat, 15 Jan 2022 20:06:03: 15000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:06:09: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:06:09: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:06:09: #1 total tags in treatment: 7855735 INFO @ Sat, 15 Jan 2022 20:06:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:06:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:06:09: #1 tags after filtering in treatment: 5261270 INFO @ Sat, 15 Jan 2022 20:06:09: #1 Redundant rate of treatment: 0.33 INFO @ Sat, 15 Jan 2022 20:06:09: #1 finished! INFO @ Sat, 15 Jan 2022 20:06:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:06:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:06:09: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:06:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:06:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144940/SRX9144940.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling