Job ID = 14520533 SRX = SRX9144935 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 12024985 spots for SRR12664293/SRR12664293.sra Written 12024985 spots for SRR12664293/SRR12664293.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:19 12024985 reads; of these: 12024985 (100.00%) were paired; of these: 740965 (6.16%) aligned concordantly 0 times 9529762 (79.25%) aligned concordantly exactly 1 time 1754258 (14.59%) aligned concordantly >1 times ---- 740965 pairs aligned concordantly 0 times; of these: 177875 (24.01%) aligned discordantly 1 time ---- 563090 pairs aligned 0 times concordantly or discordantly; of these: 1126180 mates make up the pairs; of these: 946744 (84.07%) aligned 0 times 65668 (5.83%) aligned exactly 1 time 113768 (10.10%) aligned >1 times 96.06% overall alignment rate Time searching: 00:15:19 Overall time: 00:15:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 4527368 / 11459549 = 0.3951 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:44:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:44:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:44:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:44:45: 1000000 INFO @ Sat, 15 Jan 2022 19:44:52: 2000000 INFO @ Sat, 15 Jan 2022 19:44:59: 3000000 INFO @ Sat, 15 Jan 2022 19:45:06: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:14: 5000000 INFO @ Sat, 15 Jan 2022 19:45:18: 1000000 INFO @ Sat, 15 Jan 2022 19:45:23: 6000000 INFO @ Sat, 15 Jan 2022 19:45:29: 2000000 INFO @ Sat, 15 Jan 2022 19:45:32: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:45:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:45:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:45:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:45:40: 3000000 INFO @ Sat, 15 Jan 2022 19:45:41: 8000000 INFO @ Sat, 15 Jan 2022 19:45:48: 1000000 INFO @ Sat, 15 Jan 2022 19:45:50: 9000000 INFO @ Sat, 15 Jan 2022 19:45:51: 4000000 INFO @ Sat, 15 Jan 2022 19:45:59: 2000000 INFO @ Sat, 15 Jan 2022 19:46:00: 10000000 INFO @ Sat, 15 Jan 2022 19:46:02: 5000000 INFO @ Sat, 15 Jan 2022 19:46:09: 11000000 INFO @ Sat, 15 Jan 2022 19:46:09: 3000000 INFO @ Sat, 15 Jan 2022 19:46:13: 6000000 INFO @ Sat, 15 Jan 2022 19:46:18: 12000000 INFO @ Sat, 15 Jan 2022 19:46:20: 4000000 INFO @ Sat, 15 Jan 2022 19:46:24: 7000000 INFO @ Sat, 15 Jan 2022 19:46:27: 13000000 INFO @ Sat, 15 Jan 2022 19:46:31: 5000000 INFO @ Sat, 15 Jan 2022 19:46:35: 8000000 INFO @ Sat, 15 Jan 2022 19:46:36: 14000000 INFO @ Sat, 15 Jan 2022 19:46:37: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:46:37: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:46:37: #1 total tags in treatment: 6823739 INFO @ Sat, 15 Jan 2022 19:46:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:46:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:46:37: #1 tags after filtering in treatment: 5255608 INFO @ Sat, 15 Jan 2022 19:46:37: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:46:37: #1 finished! INFO @ Sat, 15 Jan 2022 19:46:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:46:37: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:46:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:46:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:46:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:46:41: 6000000 INFO @ Sat, 15 Jan 2022 19:46:46: 9000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:46:52: 7000000 INFO @ Sat, 15 Jan 2022 19:46:57: 10000000 INFO @ Sat, 15 Jan 2022 19:47:02: 8000000 INFO @ Sat, 15 Jan 2022 19:47:08: 11000000 INFO @ Sat, 15 Jan 2022 19:47:13: 9000000 INFO @ Sat, 15 Jan 2022 19:47:19: 12000000 INFO @ Sat, 15 Jan 2022 19:47:24: 10000000 INFO @ Sat, 15 Jan 2022 19:47:30: 13000000 INFO @ Sat, 15 Jan 2022 19:47:35: 11000000 INFO @ Sat, 15 Jan 2022 19:47:40: 14000000 INFO @ Sat, 15 Jan 2022 19:47:41: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:47:41: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:47:41: #1 total tags in treatment: 6823739 INFO @ Sat, 15 Jan 2022 19:47:41: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:47:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:47:41: #1 tags after filtering in treatment: 5255608 INFO @ Sat, 15 Jan 2022 19:47:41: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:47:41: #1 finished! INFO @ Sat, 15 Jan 2022 19:47:41: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:47:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:47:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:47:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:47:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:47:46: 12000000 INFO @ Sat, 15 Jan 2022 19:47:56: 13000000 INFO @ Sat, 15 Jan 2022 19:48:05: 14000000 INFO @ Sat, 15 Jan 2022 19:48:06: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 19:48:06: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 19:48:06: #1 total tags in treatment: 6823739 INFO @ Sat, 15 Jan 2022 19:48:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:48:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:48:06: #1 tags after filtering in treatment: 5255608 INFO @ Sat, 15 Jan 2022 19:48:06: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 19:48:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:48:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:48:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:48:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:48:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:48:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144935/SRX9144935.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling