Job ID = 14520532 SRX = SRX9144934 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13952037 spots for SRR12664292/SRR12664292.sra Written 13952037 spots for SRR12664292/SRR12664292.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:19 13952037 reads; of these: 13952037 (100.00%) were paired; of these: 826746 (5.93%) aligned concordantly 0 times 10839650 (77.69%) aligned concordantly exactly 1 time 2285641 (16.38%) aligned concordantly >1 times ---- 826746 pairs aligned concordantly 0 times; of these: 228447 (27.63%) aligned discordantly 1 time ---- 598299 pairs aligned 0 times concordantly or discordantly; of these: 1196598 mates make up the pairs; of these: 979960 (81.90%) aligned 0 times 65670 (5.49%) aligned exactly 1 time 150968 (12.62%) aligned >1 times 96.49% overall alignment rate Time searching: 00:44:19 Overall time: 00:44:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5473313 / 13350849 = 0.4100 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:16:31: 1000000 INFO @ Sat, 15 Jan 2022 20:16:38: 2000000 INFO @ Sat, 15 Jan 2022 20:16:46: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:16:53: 4000000 INFO @ Sat, 15 Jan 2022 20:16:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:16:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:16:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:01: 5000000 INFO @ Sat, 15 Jan 2022 20:17:03: 1000000 INFO @ Sat, 15 Jan 2022 20:17:09: 6000000 INFO @ Sat, 15 Jan 2022 20:17:12: 2000000 INFO @ Sat, 15 Jan 2022 20:17:17: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 20:17:22: 3000000 INFO @ Sat, 15 Jan 2022 20:17:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 20:17:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 20:17:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 20:17:25: 8000000 INFO @ Sat, 15 Jan 2022 20:17:32: 4000000 INFO @ Sat, 15 Jan 2022 20:17:33: 1000000 INFO @ Sat, 15 Jan 2022 20:17:34: 9000000 INFO @ Sat, 15 Jan 2022 20:17:41: 2000000 INFO @ Sat, 15 Jan 2022 20:17:42: 5000000 INFO @ Sat, 15 Jan 2022 20:17:43: 10000000 INFO @ Sat, 15 Jan 2022 20:17:50: 3000000 INFO @ Sat, 15 Jan 2022 20:17:51: 11000000 INFO @ Sat, 15 Jan 2022 20:17:53: 6000000 INFO @ Sat, 15 Jan 2022 20:17:59: 4000000 INFO @ Sat, 15 Jan 2022 20:18:00: 12000000 INFO @ Sat, 15 Jan 2022 20:18:04: 7000000 INFO @ Sat, 15 Jan 2022 20:18:08: 5000000 INFO @ Sat, 15 Jan 2022 20:18:09: 13000000 INFO @ Sat, 15 Jan 2022 20:18:15: 8000000 INFO @ Sat, 15 Jan 2022 20:18:17: 6000000 INFO @ Sat, 15 Jan 2022 20:18:17: 14000000 INFO @ Sat, 15 Jan 2022 20:18:26: 7000000 INFO @ Sat, 15 Jan 2022 20:18:26: 15000000 INFO @ Sat, 15 Jan 2022 20:18:27: 9000000 INFO @ Sat, 15 Jan 2022 20:18:34: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:18:34: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:18:34: #1 total tags in treatment: 7740624 INFO @ Sat, 15 Jan 2022 20:18:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:18:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:18:34: 8000000 INFO @ Sat, 15 Jan 2022 20:18:34: #1 tags after filtering in treatment: 5716274 INFO @ Sat, 15 Jan 2022 20:18:34: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:18:34: #1 finished! INFO @ Sat, 15 Jan 2022 20:18:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:18:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:18:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:18:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:18:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:18:35: 10000000 INFO @ Sat, 15 Jan 2022 20:18:42: 9000000 INFO @ Sat, 15 Jan 2022 20:18:44: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 20:18:49: 10000000 INFO @ Sat, 15 Jan 2022 20:18:53: 12000000 INFO @ Sat, 15 Jan 2022 20:18:57: 11000000 INFO @ Sat, 15 Jan 2022 20:19:02: 13000000 INFO @ Sat, 15 Jan 2022 20:19:05: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 20:19:11: 14000000 INFO @ Sat, 15 Jan 2022 20:19:13: 13000000 INFO @ Sat, 15 Jan 2022 20:19:20: 15000000 INFO @ Sat, 15 Jan 2022 20:19:21: 14000000 INFO @ Sat, 15 Jan 2022 20:19:29: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:19:29: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:19:29: #1 total tags in treatment: 7740624 INFO @ Sat, 15 Jan 2022 20:19:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:29: #1 tags after filtering in treatment: 5716274 INFO @ Sat, 15 Jan 2022 20:19:29: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:19:29: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:29: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:29: 15000000 INFO @ Sat, 15 Jan 2022 20:19:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 20:19:36: #1 tag size is determined as 151 bps INFO @ Sat, 15 Jan 2022 20:19:36: #1 tag size = 151 INFO @ Sat, 15 Jan 2022 20:19:36: #1 total tags in treatment: 7740624 INFO @ Sat, 15 Jan 2022 20:19:36: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 20:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 20:19:36: #1 tags after filtering in treatment: 5716274 INFO @ Sat, 15 Jan 2022 20:19:36: #1 Redundant rate of treatment: 0.26 INFO @ Sat, 15 Jan 2022 20:19:36: #1 finished! INFO @ Sat, 15 Jan 2022 20:19:36: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 20:19:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 20:19:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 20:19:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 20:19:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9144934/SRX9144934.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling