Job ID = 14519740 SRX = SRX9067191 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 8212994 spots for SRR12580344/SRR12580344.sra Written 8212994 spots for SRR12580344/SRR12580344.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:29 8212994 reads; of these: 8212994 (100.00%) were paired; of these: 1424221 (17.34%) aligned concordantly 0 times 5223072 (63.60%) aligned concordantly exactly 1 time 1565701 (19.06%) aligned concordantly >1 times ---- 1424221 pairs aligned concordantly 0 times; of these: 74243 (5.21%) aligned discordantly 1 time ---- 1349978 pairs aligned 0 times concordantly or discordantly; of these: 2699956 mates make up the pairs; of these: 2570438 (95.20%) aligned 0 times 76133 (2.82%) aligned exactly 1 time 53385 (1.98%) aligned >1 times 84.35% overall alignment rate Time searching: 00:05:29 Overall time: 00:05:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3293727 / 6848263 = 0.4810 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:44:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:44:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:44:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:45:01: 1000000 INFO @ Sat, 15 Jan 2022 17:45:08: 2000000 INFO @ Sat, 15 Jan 2022 17:45:16: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:45:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:45:23: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:45:23: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:45:23: 4000000 INFO @ Sat, 15 Jan 2022 17:45:31: 5000000 INFO @ Sat, 15 Jan 2022 17:45:31: 1000000 INFO @ Sat, 15 Jan 2022 17:45:39: 6000000 INFO @ Sat, 15 Jan 2022 17:45:39: 2000000 INFO @ Sat, 15 Jan 2022 17:45:47: 7000000 INFO @ Sat, 15 Jan 2022 17:45:47: 3000000 INFO @ Sat, 15 Jan 2022 17:45:48: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:45:48: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:45:48: #1 total tags in treatment: 3507919 INFO @ Sat, 15 Jan 2022 17:45:48: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:49: #1 tags after filtering in treatment: 2418938 INFO @ Sat, 15 Jan 2022 17:45:49: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 17:45:49: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:49: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:49: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 17:45:49: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:45:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:45:53: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:45:53: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:45:55: 4000000 INFO @ Sat, 15 Jan 2022 17:46:01: 1000000 INFO @ Sat, 15 Jan 2022 17:46:02: 5000000 INFO @ Sat, 15 Jan 2022 17:46:08: 2000000 INFO @ Sat, 15 Jan 2022 17:46:10: 6000000 INFO @ Sat, 15 Jan 2022 17:46:16: 3000000 INFO @ Sat, 15 Jan 2022 17:46:18: 7000000 INFO @ Sat, 15 Jan 2022 17:46:19: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:46:19: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:46:19: #1 total tags in treatment: 3507919 INFO @ Sat, 15 Jan 2022 17:46:19: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:19: #1 tags after filtering in treatment: 2418938 INFO @ Sat, 15 Jan 2022 17:46:19: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 17:46:19: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:19: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:20: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 17:46:20: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:46:24: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:46:32: 5000000 INFO @ Sat, 15 Jan 2022 17:46:40: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:46:48: 7000000 INFO @ Sat, 15 Jan 2022 17:46:50: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 17:46:50: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 17:46:50: #1 total tags in treatment: 3507919 INFO @ Sat, 15 Jan 2022 17:46:50: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:50: #1 tags after filtering in treatment: 2418938 INFO @ Sat, 15 Jan 2022 17:46:50: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Jan 2022 17:46:50: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:50: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:50: #2 number of paired peaks: 27 WARNING @ Sat, 15 Jan 2022 17:46:50: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067191/SRX9067191.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling