Job ID = 14519699 SRX = SRX9067181 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 9235115 spots for SRR12580334/SRR12580334.sra Written 9235115 spots for SRR12580334/SRR12580334.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:15 9235115 reads; of these: 9235115 (100.00%) were paired; of these: 1084564 (11.74%) aligned concordantly 0 times 6453070 (69.88%) aligned concordantly exactly 1 time 1697481 (18.38%) aligned concordantly >1 times ---- 1084564 pairs aligned concordantly 0 times; of these: 164724 (15.19%) aligned discordantly 1 time ---- 919840 pairs aligned 0 times concordantly or discordantly; of these: 1839680 mates make up the pairs; of these: 1361134 (73.99%) aligned 0 times 320223 (17.41%) aligned exactly 1 time 158323 (8.61%) aligned >1 times 92.63% overall alignment rate Time searching: 00:04:15 Overall time: 00:04:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1926499 / 8294811 = 0.2323 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:36:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:36:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:36:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:37:02: 1000000 INFO @ Sat, 15 Jan 2022 17:37:08: 2000000 INFO @ Sat, 15 Jan 2022 17:37:14: 3000000 INFO @ Sat, 15 Jan 2022 17:37:21: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:37:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:37:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:37:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:37:27: 5000000 INFO @ Sat, 15 Jan 2022 17:37:33: 1000000 INFO @ Sat, 15 Jan 2022 17:37:34: 6000000 INFO @ Sat, 15 Jan 2022 17:37:40: 2000000 INFO @ Sat, 15 Jan 2022 17:37:42: 7000000 INFO @ Sat, 15 Jan 2022 17:37:46: 3000000 INFO @ Sat, 15 Jan 2022 17:37:49: 8000000 INFO @ Sat, 15 Jan 2022 17:37:53: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:37:56: 9000000 INFO @ Sat, 15 Jan 2022 17:37:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:37:56: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:37:56: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:38:00: 5000000 INFO @ Sat, 15 Jan 2022 17:38:03: 1000000 INFO @ Sat, 15 Jan 2022 17:38:03: 10000000 INFO @ Sat, 15 Jan 2022 17:38:07: 6000000 INFO @ Sat, 15 Jan 2022 17:38:10: 2000000 INFO @ Sat, 15 Jan 2022 17:38:10: 11000000 INFO @ Sat, 15 Jan 2022 17:38:14: 7000000 INFO @ Sat, 15 Jan 2022 17:38:17: 3000000 INFO @ Sat, 15 Jan 2022 17:38:18: 12000000 INFO @ Sat, 15 Jan 2022 17:38:21: 8000000 INFO @ Sat, 15 Jan 2022 17:38:24: 4000000 INFO @ Sat, 15 Jan 2022 17:38:25: 13000000 INFO @ Sat, 15 Jan 2022 17:38:26: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:38:26: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:38:26: #1 total tags in treatment: 6236493 INFO @ Sat, 15 Jan 2022 17:38:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:38:26: #1 tags after filtering in treatment: 3875906 INFO @ Sat, 15 Jan 2022 17:38:26: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:38:26: #1 finished! INFO @ Sat, 15 Jan 2022 17:38:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:38:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:38:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:38:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:38:27: 9000000 INFO @ Sat, 15 Jan 2022 17:38:31: 5000000 INFO @ Sat, 15 Jan 2022 17:38:34: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:38:38: 6000000 INFO @ Sat, 15 Jan 2022 17:38:41: 11000000 INFO @ Sat, 15 Jan 2022 17:38:46: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:38:47: 12000000 INFO @ Sat, 15 Jan 2022 17:38:53: 8000000 INFO @ Sat, 15 Jan 2022 17:38:54: 13000000 INFO @ Sat, 15 Jan 2022 17:38:56: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:38:56: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:38:56: #1 total tags in treatment: 6236493 INFO @ Sat, 15 Jan 2022 17:38:56: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:38:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:38:56: #1 tags after filtering in treatment: 3875906 INFO @ Sat, 15 Jan 2022 17:38:56: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:38:56: #1 finished! INFO @ Sat, 15 Jan 2022 17:38:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:38:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:38:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:38:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:38:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:38:59: 9000000 INFO @ Sat, 15 Jan 2022 17:39:06: 10000000 INFO @ Sat, 15 Jan 2022 17:39:12: 11000000 INFO @ Sat, 15 Jan 2022 17:39:19: 12000000 INFO @ Sat, 15 Jan 2022 17:39:25: 13000000 INFO @ Sat, 15 Jan 2022 17:39:27: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:39:27: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:39:27: #1 total tags in treatment: 6236493 INFO @ Sat, 15 Jan 2022 17:39:27: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:39:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:39:27: #1 tags after filtering in treatment: 3875906 INFO @ Sat, 15 Jan 2022 17:39:27: #1 Redundant rate of treatment: 0.38 INFO @ Sat, 15 Jan 2022 17:39:27: #1 finished! INFO @ Sat, 15 Jan 2022 17:39:27: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:39:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:39:27: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:39:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:39:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067181/SRX9067181.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling