Job ID = 14519698 SRX = SRX9067180 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10801461 spots for SRR12580333/SRR12580333.sra Written 10801461 spots for SRR12580333/SRR12580333.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:07 10801461 reads; of these: 10801461 (100.00%) were paired; of these: 789860 (7.31%) aligned concordantly 0 times 8228307 (76.18%) aligned concordantly exactly 1 time 1783294 (16.51%) aligned concordantly >1 times ---- 789860 pairs aligned concordantly 0 times; of these: 104366 (13.21%) aligned discordantly 1 time ---- 685494 pairs aligned 0 times concordantly or discordantly; of these: 1370988 mates make up the pairs; of these: 1021945 (74.54%) aligned 0 times 252923 (18.45%) aligned exactly 1 time 96120 (7.01%) aligned >1 times 95.27% overall alignment rate Time searching: 00:07:07 Overall time: 00:07:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2250065 / 10083985 = 0.2231 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:42:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:42:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:42:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:42:58: 1000000 INFO @ Sat, 15 Jan 2022 17:43:06: 2000000 INFO @ Sat, 15 Jan 2022 17:43:16: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:43:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:43:19: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:43:19: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:43:25: 4000000 INFO @ Sat, 15 Jan 2022 17:43:29: 1000000 INFO @ Sat, 15 Jan 2022 17:43:35: 5000000 INFO @ Sat, 15 Jan 2022 17:43:39: 2000000 INFO @ Sat, 15 Jan 2022 17:43:44: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:43:49: 3000000 INFO @ Sat, 15 Jan 2022 17:43:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:43:49: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:43:49: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:43:54: 7000000 INFO @ Sat, 15 Jan 2022 17:43:59: 1000000 INFO @ Sat, 15 Jan 2022 17:44:00: 4000000 INFO @ Sat, 15 Jan 2022 17:44:04: 8000000 INFO @ Sat, 15 Jan 2022 17:44:08: 2000000 INFO @ Sat, 15 Jan 2022 17:44:10: 5000000 INFO @ Sat, 15 Jan 2022 17:44:13: 9000000 INFO @ Sat, 15 Jan 2022 17:44:18: 3000000 INFO @ Sat, 15 Jan 2022 17:44:20: 6000000 INFO @ Sat, 15 Jan 2022 17:44:23: 10000000 INFO @ Sat, 15 Jan 2022 17:44:28: 4000000 INFO @ Sat, 15 Jan 2022 17:44:30: 7000000 INFO @ Sat, 15 Jan 2022 17:44:33: 11000000 INFO @ Sat, 15 Jan 2022 17:44:37: 5000000 INFO @ Sat, 15 Jan 2022 17:44:39: 8000000 INFO @ Sat, 15 Jan 2022 17:44:42: 12000000 INFO @ Sat, 15 Jan 2022 17:44:47: 6000000 INFO @ Sat, 15 Jan 2022 17:44:50: 9000000 INFO @ Sat, 15 Jan 2022 17:44:51: 13000000 INFO @ Sat, 15 Jan 2022 17:44:56: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:45:00: 14000000 INFO @ Sat, 15 Jan 2022 17:45:01: 10000000 INFO @ Sat, 15 Jan 2022 17:45:06: 8000000 INFO @ Sat, 15 Jan 2022 17:45:10: 15000000 INFO @ Sat, 15 Jan 2022 17:45:12: 11000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:45:15: 9000000 INFO @ Sat, 15 Jan 2022 17:45:20: 16000000 INFO @ Sat, 15 Jan 2022 17:45:20: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:45:20: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:45:20: #1 total tags in treatment: 7766127 INFO @ Sat, 15 Jan 2022 17:45:20: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:45:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:45:21: #1 tags after filtering in treatment: 4477630 INFO @ Sat, 15 Jan 2022 17:45:21: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 17:45:21: #1 finished! INFO @ Sat, 15 Jan 2022 17:45:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:45:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:45:21: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:45:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:45:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:45:22: 12000000 INFO @ Sat, 15 Jan 2022 17:45:25: 10000000 INFO @ Sat, 15 Jan 2022 17:45:33: 13000000 INFO @ Sat, 15 Jan 2022 17:45:34: 11000000 INFO @ Sat, 15 Jan 2022 17:45:43: 12000000 INFO @ Sat, 15 Jan 2022 17:45:44: 14000000 INFO @ Sat, 15 Jan 2022 17:45:52: 13000000 INFO @ Sat, 15 Jan 2022 17:45:54: 15000000 INFO @ Sat, 15 Jan 2022 17:46:01: 14000000 INFO @ Sat, 15 Jan 2022 17:46:04: 16000000 INFO @ Sat, 15 Jan 2022 17:46:05: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:46:05: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:46:05: #1 total tags in treatment: 7766127 INFO @ Sat, 15 Jan 2022 17:46:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:05: #1 tags after filtering in treatment: 4477630 INFO @ Sat, 15 Jan 2022 17:46:05: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 17:46:05: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:06: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:46:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:46:11: 15000000 INFO @ Sat, 15 Jan 2022 17:46:21: 16000000 INFO @ Sat, 15 Jan 2022 17:46:21: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:46:21: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:46:21: #1 total tags in treatment: 7766127 INFO @ Sat, 15 Jan 2022 17:46:21: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:46:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:46:21: #1 tags after filtering in treatment: 4477630 INFO @ Sat, 15 Jan 2022 17:46:21: #1 Redundant rate of treatment: 0.42 INFO @ Sat, 15 Jan 2022 17:46:21: #1 finished! INFO @ Sat, 15 Jan 2022 17:46:21: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:46:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:46:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:46:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:46:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067180/SRX9067180.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling