Job ID = 14519687 SRX = SRX9067176 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4124072 spots for SRR12580329/SRR12580329.sra Written 4124072 spots for SRR12580329/SRR12580329.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 4124072 reads; of these: 4124072 (100.00%) were paired; of these: 442117 (10.72%) aligned concordantly 0 times 3419536 (82.92%) aligned concordantly exactly 1 time 262419 (6.36%) aligned concordantly >1 times ---- 442117 pairs aligned concordantly 0 times; of these: 148379 (33.56%) aligned discordantly 1 time ---- 293738 pairs aligned 0 times concordantly or discordantly; of these: 587476 mates make up the pairs; of these: 371299 (63.20%) aligned 0 times 180223 (30.68%) aligned exactly 1 time 35954 (6.12%) aligned >1 times 95.50% overall alignment rate Time searching: 00:02:40 Overall time: 00:02:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 250591 / 3751561 = 0.0668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:28:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:28:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:28:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:28:18: 1000000 INFO @ Sat, 15 Jan 2022 17:28:25: 2000000 INFO @ Sat, 15 Jan 2022 17:28:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:28:39: 4000000 INFO @ Sat, 15 Jan 2022 17:28:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:28:40: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:28:40: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:28:47: 1000000 INFO @ Sat, 15 Jan 2022 17:28:47: 5000000 INFO @ Sat, 15 Jan 2022 17:28:53: 2000000 INFO @ Sat, 15 Jan 2022 17:28:54: 6000000 INFO @ Sat, 15 Jan 2022 17:29:00: 3000000 INFO @ Sat, 15 Jan 2022 17:29:02: 7000000 INFO @ Sat, 15 Jan 2022 17:29:05: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:29:05: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:29:05: #1 total tags in treatment: 3433532 INFO @ Sat, 15 Jan 2022 17:29:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:29:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:29:05: #1 tags after filtering in treatment: 2503432 INFO @ Sat, 15 Jan 2022 17:29:05: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 17:29:05: #1 finished! INFO @ Sat, 15 Jan 2022 17:29:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:29:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:29:05: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:29:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:29:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:29:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:29:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:29:10: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:29:10: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:29:12: 5000000 INFO @ Sat, 15 Jan 2022 17:29:17: 1000000 INFO @ Sat, 15 Jan 2022 17:29:18: 6000000 INFO @ Sat, 15 Jan 2022 17:29:24: 7000000 INFO @ Sat, 15 Jan 2022 17:29:24: 2000000 INFO @ Sat, 15 Jan 2022 17:29:26: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:29:26: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:29:26: #1 total tags in treatment: 3433532 INFO @ Sat, 15 Jan 2022 17:29:26: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:29:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:29:26: #1 tags after filtering in treatment: 2503432 INFO @ Sat, 15 Jan 2022 17:29:26: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 17:29:26: #1 finished! INFO @ Sat, 15 Jan 2022 17:29:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:29:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:29:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:29:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:29:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:29:32: 3000000 INFO @ Sat, 15 Jan 2022 17:29:39: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:29:46: 5000000 INFO @ Sat, 15 Jan 2022 17:29:53: 6000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:30:01: 7000000 INFO @ Sat, 15 Jan 2022 17:30:03: #1 tag size is determined as 37 bps INFO @ Sat, 15 Jan 2022 17:30:03: #1 tag size = 37 INFO @ Sat, 15 Jan 2022 17:30:03: #1 total tags in treatment: 3433532 INFO @ Sat, 15 Jan 2022 17:30:03: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:30:03: #1 tags after filtering in treatment: 2503432 INFO @ Sat, 15 Jan 2022 17:30:03: #1 Redundant rate of treatment: 0.27 INFO @ Sat, 15 Jan 2022 17:30:03: #1 finished! INFO @ Sat, 15 Jan 2022 17:30:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:30:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:30:04: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:30:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:30:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067176/SRX9067176.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling