Job ID = 14519653 SRX = SRX9067166 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13183099 spots for SRR12580319/SRR12580319.sra Written 13183099 spots for SRR12580319/SRR12580319.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:11 13183099 reads; of these: 13183099 (100.00%) were paired; of these: 4096186 (31.07%) aligned concordantly 0 times 8020893 (60.84%) aligned concordantly exactly 1 time 1066020 (8.09%) aligned concordantly >1 times ---- 4096186 pairs aligned concordantly 0 times; of these: 536015 (13.09%) aligned discordantly 1 time ---- 3560171 pairs aligned 0 times concordantly or discordantly; of these: 7120342 mates make up the pairs; of these: 6260968 (87.93%) aligned 0 times 650868 (9.14%) aligned exactly 1 time 208506 (2.93%) aligned >1 times 76.25% overall alignment rate Time searching: 00:07:11 Overall time: 00:07:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 816069 / 9468369 = 0.0862 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:28:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:28:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:28:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:28:15: 1000000 INFO @ Sat, 15 Jan 2022 17:28:22: 2000000 INFO @ Sat, 15 Jan 2022 17:28:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:28:35: 4000000 INFO @ Sat, 15 Jan 2022 17:28:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:28:37: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:28:37: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:28:42: 5000000 INFO @ Sat, 15 Jan 2022 17:28:45: 1000000 INFO @ Sat, 15 Jan 2022 17:28:49: 6000000 INFO @ Sat, 15 Jan 2022 17:28:52: 2000000 INFO @ Sat, 15 Jan 2022 17:28:56: 7000000 INFO @ Sat, 15 Jan 2022 17:28:59: 3000000 INFO @ Sat, 15 Jan 2022 17:29:04: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 17:29:07: 4000000 INFO @ Sat, 15 Jan 2022 17:29:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 17:29:07: #1 read tag files... INFO @ Sat, 15 Jan 2022 17:29:07: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 17:29:11: 9000000 INFO @ Sat, 15 Jan 2022 17:29:14: 5000000 INFO @ Sat, 15 Jan 2022 17:29:16: 1000000 INFO @ Sat, 15 Jan 2022 17:29:19: 10000000 INFO @ Sat, 15 Jan 2022 17:29:21: 6000000 INFO @ Sat, 15 Jan 2022 17:29:24: 2000000 INFO @ Sat, 15 Jan 2022 17:29:26: 11000000 INFO @ Sat, 15 Jan 2022 17:29:29: 7000000 INFO @ Sat, 15 Jan 2022 17:29:32: 3000000 INFO @ Sat, 15 Jan 2022 17:29:33: 12000000 INFO @ Sat, 15 Jan 2022 17:29:36: 8000000 INFO @ Sat, 15 Jan 2022 17:29:39: 4000000 INFO @ Sat, 15 Jan 2022 17:29:41: 13000000 INFO @ Sat, 15 Jan 2022 17:29:44: 9000000 INFO @ Sat, 15 Jan 2022 17:29:46: 5000000 INFO @ Sat, 15 Jan 2022 17:29:49: 14000000 INFO @ Sat, 15 Jan 2022 17:29:52: 10000000 INFO @ Sat, 15 Jan 2022 17:29:54: 6000000 INFO @ Sat, 15 Jan 2022 17:29:57: 15000000 INFO @ Sat, 15 Jan 2022 17:29:59: 11000000 INFO @ Sat, 15 Jan 2022 17:30:01: 7000000 INFO @ Sat, 15 Jan 2022 17:30:04: 16000000 INFO @ Sat, 15 Jan 2022 17:30:06: 12000000 INFO @ Sat, 15 Jan 2022 17:30:09: 8000000 INFO @ Sat, 15 Jan 2022 17:30:11: 17000000 INFO @ Sat, 15 Jan 2022 17:30:13: 13000000 INFO @ Sat, 15 Jan 2022 17:30:18: 9000000 INFO @ Sat, 15 Jan 2022 17:30:19: 18000000 INFO @ Sat, 15 Jan 2022 17:30:21: 14000000 INFO @ Sat, 15 Jan 2022 17:30:23: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 17:30:23: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 17:30:23: #1 total tags in treatment: 8289693 INFO @ Sat, 15 Jan 2022 17:30:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:30:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:30:23: #1 tags after filtering in treatment: 4731176 INFO @ Sat, 15 Jan 2022 17:30:23: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:30:23: #1 finished! INFO @ Sat, 15 Jan 2022 17:30:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:30:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:30:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:30:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:30:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:30:27: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 17:30:29: 15000000 INFO @ Sat, 15 Jan 2022 17:30:35: 11000000 INFO @ Sat, 15 Jan 2022 17:30:36: 16000000 INFO @ Sat, 15 Jan 2022 17:30:43: 17000000 INFO @ Sat, 15 Jan 2022 17:30:43: 12000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 17:30:50: 18000000 INFO @ Sat, 15 Jan 2022 17:30:51: 13000000 INFO @ Sat, 15 Jan 2022 17:30:53: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 17:30:53: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 17:30:53: #1 total tags in treatment: 8289693 INFO @ Sat, 15 Jan 2022 17:30:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:30:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:30:54: #1 tags after filtering in treatment: 4731176 INFO @ Sat, 15 Jan 2022 17:30:54: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:30:54: #1 finished! INFO @ Sat, 15 Jan 2022 17:30:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:30:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:30:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:30:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:30:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 17:30:59: 14000000 INFO @ Sat, 15 Jan 2022 17:31:07: 15000000 INFO @ Sat, 15 Jan 2022 17:31:14: 16000000 INFO @ Sat, 15 Jan 2022 17:31:22: 17000000 INFO @ Sat, 15 Jan 2022 17:31:29: 18000000 INFO @ Sat, 15 Jan 2022 17:31:33: #1 tag size is determined as 45 bps INFO @ Sat, 15 Jan 2022 17:31:33: #1 tag size = 45 INFO @ Sat, 15 Jan 2022 17:31:33: #1 total tags in treatment: 8289693 INFO @ Sat, 15 Jan 2022 17:31:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 17:31:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 17:31:33: #1 tags after filtering in treatment: 4731176 INFO @ Sat, 15 Jan 2022 17:31:33: #1 Redundant rate of treatment: 0.43 INFO @ Sat, 15 Jan 2022 17:31:33: #1 finished! INFO @ Sat, 15 Jan 2022 17:31:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 17:31:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 17:31:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 17:31:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 17:31:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067166/SRX9067166.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling