Job ID = 14522136 SRX = SRX9067151 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3660782 spots for SRR12580304/SRR12580304.sra Written 3660782 spots for SRR12580304/SRR12580304.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 3660782 reads; of these: 3660782 (100.00%) were paired; of these: 913144 (24.94%) aligned concordantly 0 times 2329550 (63.64%) aligned concordantly exactly 1 time 418088 (11.42%) aligned concordantly >1 times ---- 913144 pairs aligned concordantly 0 times; of these: 165554 (18.13%) aligned discordantly 1 time ---- 747590 pairs aligned 0 times concordantly or discordantly; of these: 1495180 mates make up the pairs; of these: 1230395 (82.29%) aligned 0 times 178712 (11.95%) aligned exactly 1 time 86073 (5.76%) aligned >1 times 83.19% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 251342 / 2794294 = 0.0899 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:18:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:18:09: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:18:09: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:18:18: 1000000 INFO @ Sat, 15 Jan 2022 22:18:28: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:18:38: 3000000 INFO @ Sat, 15 Jan 2022 22:18:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:18:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:18:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:18:47: 1000000 INFO @ Sat, 15 Jan 2022 22:18:48: 4000000 INFO @ Sat, 15 Jan 2022 22:18:56: 2000000 INFO @ Sat, 15 Jan 2022 22:18:59: 5000000 INFO @ Sat, 15 Jan 2022 22:19:05: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:19:05: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:19:05: #1 total tags in treatment: 2497453 INFO @ Sat, 15 Jan 2022 22:19:05: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:19:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:19:05: #1 tags after filtering in treatment: 1923400 INFO @ Sat, 15 Jan 2022 22:19:05: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:19:05: #1 finished! INFO @ Sat, 15 Jan 2022 22:19:05: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:19:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:19:05: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:19:05: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:19:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:19:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:19:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:19:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:19:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:19:14: 4000000 INFO @ Sat, 15 Jan 2022 22:19:19: 1000000 INFO @ Sat, 15 Jan 2022 22:19:23: 5000000 INFO @ Sat, 15 Jan 2022 22:19:28: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:19:28: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:19:28: #1 total tags in treatment: 2497453 INFO @ Sat, 15 Jan 2022 22:19:28: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:19:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:19:28: #1 tags after filtering in treatment: 1923400 INFO @ Sat, 15 Jan 2022 22:19:28: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:19:28: #1 finished! INFO @ Sat, 15 Jan 2022 22:19:28: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:19:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:19:29: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:19:29: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:19:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:19:31: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:19:42: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:19:54: 4000000 INFO @ Sat, 15 Jan 2022 22:20:06: 5000000 INFO @ Sat, 15 Jan 2022 22:20:13: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:20:13: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:20:13: #1 total tags in treatment: 2497453 INFO @ Sat, 15 Jan 2022 22:20:13: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:20:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:20:13: #1 tags after filtering in treatment: 1923400 INFO @ Sat, 15 Jan 2022 22:20:13: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 15 Jan 2022 22:20:13: #1 finished! INFO @ Sat, 15 Jan 2022 22:20:13: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:20:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:20:13: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:20:13: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:20:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067151/SRX9067151.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling