Job ID = 14522117 SRX = SRX9067149 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3184154 spots for SRR12580302/SRR12580302.sra Written 3184154 spots for SRR12580302/SRR12580302.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:29 3184154 reads; of these: 3184154 (100.00%) were paired; of these: 573264 (18.00%) aligned concordantly 0 times 2286121 (71.80%) aligned concordantly exactly 1 time 324769 (10.20%) aligned concordantly >1 times ---- 573264 pairs aligned concordantly 0 times; of these: 225345 (39.31%) aligned discordantly 1 time ---- 347919 pairs aligned 0 times concordantly or discordantly; of these: 695838 mates make up the pairs; of these: 423871 (60.92%) aligned 0 times 183796 (26.41%) aligned exactly 1 time 88171 (12.67%) aligned >1 times 93.34% overall alignment rate Time searching: 00:01:29 Overall time: 00:01:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 185334 / 2665099 = 0.0695 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:14:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:14:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:14:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:14:36: 1000000 INFO @ Sat, 15 Jan 2022 22:14:40: 2000000 INFO @ Sat, 15 Jan 2022 22:14:44: 3000000 INFO @ Sat, 15 Jan 2022 22:14:48: 4000000 INFO @ Sat, 15 Jan 2022 22:14:52: 5000000 INFO @ Sat, 15 Jan 2022 22:14:54: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:14:54: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:14:54: #1 total tags in treatment: 2427106 INFO @ Sat, 15 Jan 2022 22:14:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:14:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:14:54: #1 tags after filtering in treatment: 1915371 INFO @ Sat, 15 Jan 2022 22:14:54: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:14:54: #1 finished! INFO @ Sat, 15 Jan 2022 22:14:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:14:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:14:54: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:14:54: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:14:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:15:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:15:02: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:15:02: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:15:07: 1000000 INFO @ Sat, 15 Jan 2022 22:15:11: 2000000 INFO @ Sat, 15 Jan 2022 22:15:15: 3000000 INFO @ Sat, 15 Jan 2022 22:15:19: 4000000 INFO @ Sat, 15 Jan 2022 22:15:23: 5000000 INFO @ Sat, 15 Jan 2022 22:15:25: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:15:25: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:15:25: #1 total tags in treatment: 2427106 INFO @ Sat, 15 Jan 2022 22:15:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:25: #1 tags after filtering in treatment: 1915371 INFO @ Sat, 15 Jan 2022 22:15:25: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:15:25: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:25: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:25: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:15:25: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:15:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:15:32: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:15:32: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:15:36: 1000000 INFO @ Sat, 15 Jan 2022 22:15:40: 2000000 INFO @ Sat, 15 Jan 2022 22:15:44: 3000000 INFO @ Sat, 15 Jan 2022 22:15:48: 4000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:15:52: 5000000 INFO @ Sat, 15 Jan 2022 22:15:54: #1 tag size is determined as 35 bps INFO @ Sat, 15 Jan 2022 22:15:54: #1 tag size = 35 INFO @ Sat, 15 Jan 2022 22:15:54: #1 total tags in treatment: 2427106 INFO @ Sat, 15 Jan 2022 22:15:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:55: #1 tags after filtering in treatment: 1915371 INFO @ Sat, 15 Jan 2022 22:15:55: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 15 Jan 2022 22:15:55: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:55: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:55: #2 number of paired peaks: 26 WARNING @ Sat, 15 Jan 2022 22:15:55: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067149/SRX9067149.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。