Job ID = 14522113 SRX = SRX9067145 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6024666 spots for SRR12580298/SRR12580298.sra Written 6024666 spots for SRR12580298/SRR12580298.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:10 6024666 reads; of these: 6024666 (100.00%) were paired; of these: 994009 (16.50%) aligned concordantly 0 times 4401361 (73.06%) aligned concordantly exactly 1 time 629296 (10.45%) aligned concordantly >1 times ---- 994009 pairs aligned concordantly 0 times; of these: 230036 (23.14%) aligned discordantly 1 time ---- 763973 pairs aligned 0 times concordantly or discordantly; of these: 1527946 mates make up the pairs; of these: 1264859 (82.78%) aligned 0 times 179016 (11.72%) aligned exactly 1 time 84071 (5.50%) aligned >1 times 89.50% overall alignment rate Time searching: 00:03:10 Overall time: 00:03:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 695348 / 5132766 = 0.1355 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:16:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:16:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:16:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:17:01: 1000000 INFO @ Sat, 15 Jan 2022 22:17:05: 2000000 INFO @ Sat, 15 Jan 2022 22:17:09: 3000000 INFO @ Sat, 15 Jan 2022 22:17:14: 4000000 INFO @ Sat, 15 Jan 2022 22:17:18: 5000000 INFO @ Sat, 15 Jan 2022 22:17:22: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:17:26: 7000000 INFO @ Sat, 15 Jan 2022 22:17:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:17:26: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:17:26: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:17:31: 8000000 INFO @ Sat, 15 Jan 2022 22:17:31: 1000000 INFO @ Sat, 15 Jan 2022 22:17:35: 9000000 INFO @ Sat, 15 Jan 2022 22:17:36: 2000000 INFO @ Sat, 15 Jan 2022 22:17:37: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 22:17:37: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 22:17:37: #1 total tags in treatment: 4342788 INFO @ Sat, 15 Jan 2022 22:17:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:17:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:17:37: #1 tags after filtering in treatment: 2953206 INFO @ Sat, 15 Jan 2022 22:17:37: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:17:37: #1 finished! INFO @ Sat, 15 Jan 2022 22:17:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:17:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:17:38: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:17:38: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:17:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:17:40: 3000000 INFO @ Sat, 15 Jan 2022 22:17:44: 4000000 INFO @ Sat, 15 Jan 2022 22:17:49: 5000000 INFO @ Sat, 15 Jan 2022 22:17:53: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:17:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:17:57: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:17:57: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:17:58: 7000000 INFO @ Sat, 15 Jan 2022 22:18:01: 1000000 INFO @ Sat, 15 Jan 2022 22:18:02: 8000000 INFO @ Sat, 15 Jan 2022 22:18:06: 2000000 INFO @ Sat, 15 Jan 2022 22:18:07: 9000000 INFO @ Sat, 15 Jan 2022 22:18:09: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 22:18:09: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 22:18:09: #1 total tags in treatment: 4342788 INFO @ Sat, 15 Jan 2022 22:18:09: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:18:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:18:09: #1 tags after filtering in treatment: 2953206 INFO @ Sat, 15 Jan 2022 22:18:09: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:18:09: #1 finished! INFO @ Sat, 15 Jan 2022 22:18:09: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:18:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:18:09: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:18:09: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:18:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:18:10: 3000000 INFO @ Sat, 15 Jan 2022 22:18:15: 4000000 INFO @ Sat, 15 Jan 2022 22:18:19: 5000000 INFO @ Sat, 15 Jan 2022 22:18:23: 6000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:18:28: 7000000 INFO @ Sat, 15 Jan 2022 22:18:32: 8000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:18:36: 9000000 INFO @ Sat, 15 Jan 2022 22:18:38: #1 tag size is determined as 49 bps INFO @ Sat, 15 Jan 2022 22:18:38: #1 tag size = 49 INFO @ Sat, 15 Jan 2022 22:18:38: #1 total tags in treatment: 4342788 INFO @ Sat, 15 Jan 2022 22:18:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:18:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:18:38: #1 tags after filtering in treatment: 2953206 INFO @ Sat, 15 Jan 2022 22:18:38: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 15 Jan 2022 22:18:38: #1 finished! INFO @ Sat, 15 Jan 2022 22:18:38: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:18:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:18:38: #2 number of paired peaks: 1 WARNING @ Sat, 15 Jan 2022 22:18:38: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:18:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067145/SRX9067145.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling