Job ID = 14522109 SRX = SRX9067141 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3871257 spots for SRR12580294/SRR12580294.sra Written 3871257 spots for SRR12580294/SRR12580294.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:46 3871257 reads; of these: 3871257 (100.00%) were paired; of these: 841456 (21.74%) aligned concordantly 0 times 2618113 (67.63%) aligned concordantly exactly 1 time 411688 (10.63%) aligned concordantly >1 times ---- 841456 pairs aligned concordantly 0 times; of these: 340229 (40.43%) aligned discordantly 1 time ---- 501227 pairs aligned 0 times concordantly or discordantly; of these: 1002454 mates make up the pairs; of these: 565065 (56.37%) aligned 0 times 288874 (28.82%) aligned exactly 1 time 148515 (14.82%) aligned >1 times 92.70% overall alignment rate Time searching: 00:01:46 Overall time: 00:01:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 262592 / 3091557 = 0.0849 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:14:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:14:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:14:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:14:27: 1000000 INFO @ Sat, 15 Jan 2022 22:14:33: 2000000 INFO @ Sat, 15 Jan 2022 22:14:39: 3000000 INFO @ Sat, 15 Jan 2022 22:14:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:14:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:14:51: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:14:51: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:14:51: 5000000 INFO @ Sat, 15 Jan 2022 22:14:57: 1000000 INFO @ Sat, 15 Jan 2022 22:14:58: 6000000 INFO @ Sat, 15 Jan 2022 22:15:02: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:15:02: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:15:02: #1 total tags in treatment: 2769043 INFO @ Sat, 15 Jan 2022 22:15:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:02: #1 tags after filtering in treatment: 2075919 INFO @ Sat, 15 Jan 2022 22:15:02: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:15:02: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:02: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:02: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 22:15:02: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:15:04: 2000000 INFO @ Sat, 15 Jan 2022 22:15:10: 3000000 INFO @ Sat, 15 Jan 2022 22:15:16: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 22:15:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 22:15:21: #1 read tag files... INFO @ Sat, 15 Jan 2022 22:15:21: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 22:15:22: 5000000 INFO @ Sat, 15 Jan 2022 22:15:27: 1000000 INFO @ Sat, 15 Jan 2022 22:15:29: 6000000 INFO @ Sat, 15 Jan 2022 22:15:33: 2000000 INFO @ Sat, 15 Jan 2022 22:15:33: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:15:33: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:15:33: #1 total tags in treatment: 2769043 INFO @ Sat, 15 Jan 2022 22:15:33: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:33: #1 tags after filtering in treatment: 2075919 INFO @ Sat, 15 Jan 2022 22:15:33: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:15:33: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:33: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:33: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 22:15:33: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 22:15:39: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 22:15:44: 4000000 INFO @ Sat, 15 Jan 2022 22:15:49: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 22:15:54: 6000000 INFO @ Sat, 15 Jan 2022 22:15:58: #1 tag size is determined as 42 bps INFO @ Sat, 15 Jan 2022 22:15:58: #1 tag size = 42 INFO @ Sat, 15 Jan 2022 22:15:58: #1 total tags in treatment: 2769043 INFO @ Sat, 15 Jan 2022 22:15:58: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 22:15:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 22:15:58: #1 tags after filtering in treatment: 2075919 INFO @ Sat, 15 Jan 2022 22:15:58: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 15 Jan 2022 22:15:58: #1 finished! INFO @ Sat, 15 Jan 2022 22:15:58: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 22:15:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 22:15:58: #2 number of paired peaks: 31 WARNING @ Sat, 15 Jan 2022 22:15:58: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 22:15:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX9067141/SRX9067141.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling