Job ID = 14520548 SRX = SRX8960441 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 16465249 spots for SRR12466250/SRR12466250.sra Written 16465249 spots for SRR12466250/SRR12466250.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:55 16465249 reads; of these: 16465249 (100.00%) were unpaired; of these: 528062 (3.21%) aligned 0 times 13446497 (81.67%) aligned exactly 1 time 2490690 (15.13%) aligned >1 times 96.79% overall alignment rate Time searching: 00:02:55 Overall time: 00:02:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5876641 / 15937187 = 0.3687 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:28:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:28:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:28:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:28:45: 1000000 INFO @ Sat, 15 Jan 2022 19:28:52: 2000000 INFO @ Sat, 15 Jan 2022 19:28:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:29:06: 4000000 INFO @ Sat, 15 Jan 2022 19:29:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:29:08: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:29:08: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:29:13: 5000000 INFO @ Sat, 15 Jan 2022 19:29:15: 1000000 INFO @ Sat, 15 Jan 2022 19:29:21: 6000000 INFO @ Sat, 15 Jan 2022 19:29:23: 2000000 INFO @ Sat, 15 Jan 2022 19:29:29: 7000000 INFO @ Sat, 15 Jan 2022 19:29:31: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:29:37: 8000000 INFO @ Sat, 15 Jan 2022 19:29:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:29:38: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:29:38: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:29:39: 4000000 INFO @ Sat, 15 Jan 2022 19:29:45: 9000000 INFO @ Sat, 15 Jan 2022 19:29:46: 1000000 INFO @ Sat, 15 Jan 2022 19:29:47: 5000000 INFO @ Sat, 15 Jan 2022 19:29:52: 10000000 INFO @ Sat, 15 Jan 2022 19:29:53: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:29:53: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:29:53: #1 total tags in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:29:53: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:29:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:29:53: #1 tags after filtering in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:29:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:29:53: #1 finished! INFO @ Sat, 15 Jan 2022 19:29:53: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:29:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:29:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:29:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:29:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:29:54: 2000000 INFO @ Sat, 15 Jan 2022 19:29:55: 6000000 INFO @ Sat, 15 Jan 2022 19:30:02: 3000000 INFO @ Sat, 15 Jan 2022 19:30:02: 7000000 INFO @ Sat, 15 Jan 2022 19:30:09: 4000000 INFO @ Sat, 15 Jan 2022 19:30:10: 8000000 INFO @ Sat, 15 Jan 2022 19:30:17: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:30:18: 9000000 INFO @ Sat, 15 Jan 2022 19:30:25: 6000000 INFO @ Sat, 15 Jan 2022 19:30:25: 10000000 INFO @ Sat, 15 Jan 2022 19:30:25: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:30:25: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:30:25: #1 total tags in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:30:25: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:26: #1 tags after filtering in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:30:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:30:26: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:26: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:26: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:30:32: 7000000 INFO @ Sat, 15 Jan 2022 19:30:39: 8000000 INFO @ Sat, 15 Jan 2022 19:30:47: 9000000 INFO @ Sat, 15 Jan 2022 19:30:55: 10000000 INFO @ Sat, 15 Jan 2022 19:30:55: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:30:55: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:30:55: #1 total tags in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:30:55: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:30:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:30:56: #1 tags after filtering in treatment: 10060546 INFO @ Sat, 15 Jan 2022 19:30:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:30:56: #1 finished! INFO @ Sat, 15 Jan 2022 19:30:56: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:30:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:30:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:30:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:30:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960441/SRX8960441.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling