Job ID = 14520503 SRX = SRX8960438 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12922529 spots for SRR12466247/SRR12466247.sra Written 12922529 spots for SRR12466247/SRR12466247.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:57 12922529 reads; of these: 12922529 (100.00%) were unpaired; of these: 497266 (3.85%) aligned 0 times 10915201 (84.47%) aligned exactly 1 time 1510062 (11.69%) aligned >1 times 96.15% overall alignment rate Time searching: 00:02:57 Overall time: 00:02:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5053442 / 12425263 = 0.4067 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:24:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:24:24: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:24:24: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:24:34: 1000000 INFO @ Sat, 15 Jan 2022 19:24:44: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:24:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:24:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:24:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:24:55: 3000000 INFO @ Sat, 15 Jan 2022 19:25:03: 1000000 INFO @ Sat, 15 Jan 2022 19:25:04: 4000000 INFO @ Sat, 15 Jan 2022 19:25:12: 2000000 INFO @ Sat, 15 Jan 2022 19:25:14: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:25:21: 3000000 INFO @ Sat, 15 Jan 2022 19:25:22: 6000000 INFO @ Sat, 15 Jan 2022 19:25:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:25:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:25:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:25:30: 4000000 INFO @ Sat, 15 Jan 2022 19:25:31: 7000000 INFO @ Sat, 15 Jan 2022 19:25:33: 1000000 INFO @ Sat, 15 Jan 2022 19:25:34: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:25:34: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:25:34: #1 total tags in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:25:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:25:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:25:35: #1 tags after filtering in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:25:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:25:35: #1 finished! INFO @ Sat, 15 Jan 2022 19:25:35: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:25:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:25:35: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:25:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:25:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:25:38: 5000000 INFO @ Sat, 15 Jan 2022 19:25:41: 2000000 INFO @ Sat, 15 Jan 2022 19:25:48: 6000000 INFO @ Sat, 15 Jan 2022 19:25:51: 3000000 INFO @ Sat, 15 Jan 2022 19:26:01: 7000000 INFO @ Sat, 15 Jan 2022 19:26:05: 4000000 INFO @ Sat, 15 Jan 2022 19:26:06: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:26:06: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:26:06: #1 total tags in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:26:06: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:26:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:26:06: #1 tags after filtering in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:26:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:26:06: #1 finished! INFO @ Sat, 15 Jan 2022 19:26:06: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:26:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:26:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:26:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:26:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:26:16: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 19:26:30: 6000000 INFO @ Sat, 15 Jan 2022 19:26:41: 7000000 INFO @ Sat, 15 Jan 2022 19:26:45: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:26:45: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:26:45: #1 total tags in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:26:45: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:26:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:26:45: #1 tags after filtering in treatment: 7371821 INFO @ Sat, 15 Jan 2022 19:26:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:26:45: #1 finished! INFO @ Sat, 15 Jan 2022 19:26:45: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:26:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:26:46: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:26:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:26:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960438/SRX8960438.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling