Job ID = 14520502 SRX = SRX8960437 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 12992093 spots for SRR12466246/SRR12466246.sra Written 12992093 spots for SRR12466246/SRR12466246.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 12992093 reads; of these: 12992093 (100.00%) were unpaired; of these: 734105 (5.65%) aligned 0 times 10381605 (79.91%) aligned exactly 1 time 1876383 (14.44%) aligned >1 times 94.35% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3998726 / 12257988 = 0.3262 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:20:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:20:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:20:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:20:27: 1000000 INFO @ Sat, 15 Jan 2022 19:20:32: 2000000 INFO @ Sat, 15 Jan 2022 19:20:37: 3000000 INFO @ Sat, 15 Jan 2022 19:20:43: 4000000 INFO @ Sat, 15 Jan 2022 19:20:48: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:20:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:20:52: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:20:52: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:20:54: 6000000 INFO @ Sat, 15 Jan 2022 19:20:57: 1000000 INFO @ Sat, 15 Jan 2022 19:21:00: 7000000 INFO @ Sat, 15 Jan 2022 19:21:03: 2000000 INFO @ Sat, 15 Jan 2022 19:21:05: 8000000 INFO @ Sat, 15 Jan 2022 19:21:07: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:21:07: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:21:07: #1 total tags in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:21:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:07: #1 tags after filtering in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:21:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:21:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:08: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:21:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:21:09: 3000000 INFO @ Sat, 15 Jan 2022 19:21:14: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 19:21:20: 5000000 INFO @ Sat, 15 Jan 2022 19:21:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 19:21:22: #1 read tag files... INFO @ Sat, 15 Jan 2022 19:21:22: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 19:21:25: 6000000 INFO @ Sat, 15 Jan 2022 19:21:27: 1000000 INFO @ Sat, 15 Jan 2022 19:21:31: 7000000 INFO @ Sat, 15 Jan 2022 19:21:33: 2000000 INFO @ Sat, 15 Jan 2022 19:21:37: 8000000 INFO @ Sat, 15 Jan 2022 19:21:38: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:21:38: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:21:38: #1 total tags in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:21:38: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:21:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:21:39: #1 tags after filtering in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:21:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:21:39: #1 finished! INFO @ Sat, 15 Jan 2022 19:21:39: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:21:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:21:39: 3000000 INFO @ Sat, 15 Jan 2022 19:21:39: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:21:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:21:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 19:21:44: 4000000 INFO @ Sat, 15 Jan 2022 19:21:50: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 19:21:55: 6000000 INFO @ Sat, 15 Jan 2022 19:22:00: 7000000 INFO @ Sat, 15 Jan 2022 19:22:05: 8000000 INFO @ Sat, 15 Jan 2022 19:22:07: #1 tag size is determined as 51 bps INFO @ Sat, 15 Jan 2022 19:22:07: #1 tag size = 51 INFO @ Sat, 15 Jan 2022 19:22:07: #1 total tags in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:22:07: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 19:22:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 19:22:07: #1 tags after filtering in treatment: 8259262 INFO @ Sat, 15 Jan 2022 19:22:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 19:22:07: #1 finished! INFO @ Sat, 15 Jan 2022 19:22:07: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 19:22:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 19:22:07: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 19:22:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 19:22:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8960437/SRX8960437.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。