Job ID = 10224079 SRX = SRX8952635 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 6992357 spots for SRR12458251/SRR12458251.sra Written 6992357 spots for SRR12458251/SRR12458251.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:58 6992357 reads; of these: 6992357 (100.00%) were paired; of these: 5681604 (81.25%) aligned concordantly 0 times 1010411 (14.45%) aligned concordantly exactly 1 time 300342 (4.30%) aligned concordantly >1 times ---- 5681604 pairs aligned concordantly 0 times; of these: 413344 (7.28%) aligned discordantly 1 time ---- 5268260 pairs aligned 0 times concordantly or discordantly; of these: 10536520 mates make up the pairs; of these: 8657227 (82.16%) aligned 0 times 1461156 (13.87%) aligned exactly 1 time 418137 (3.97%) aligned >1 times 38.10% overall alignment rate Time searching: 00:01:58 Overall time: 00:01:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 552960 / 1710655 = 0.3232 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:24:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:24:27: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:24:27: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:24:32: 1000000 INFO @ Fri, 16 Oct 2020 09:24:36: 2000000 INFO @ Fri, 16 Oct 2020 09:24:40: 3000000 INFO @ Fri, 16 Oct 2020 09:24:45: 4000000 INFO @ Fri, 16 Oct 2020 09:24:46: #1 tag size is determined as 43 bps INFO @ Fri, 16 Oct 2020 09:24:46: #1 tag size = 43 INFO @ Fri, 16 Oct 2020 09:24:46: #1 total tags in treatment: 1018891 INFO @ Fri, 16 Oct 2020 09:24:46: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:24:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:24:46: #1 tags after filtering in treatment: 796762 INFO @ Fri, 16 Oct 2020 09:24:46: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 16 Oct 2020 09:24:46: #1 finished! INFO @ Fri, 16 Oct 2020 09:24:46: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:24:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:24:46: #2 number of paired peaks: 78 WARNING @ Fri, 16 Oct 2020 09:24:46: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:24:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:24:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:24:57: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:24:57: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:25:02: 1000000 INFO @ Fri, 16 Oct 2020 09:25:06: 2000000 INFO @ Fri, 16 Oct 2020 09:25:11: 3000000 INFO @ Fri, 16 Oct 2020 09:25:15: 4000000 INFO @ Fri, 16 Oct 2020 09:25:16: #1 tag size is determined as 43 bps INFO @ Fri, 16 Oct 2020 09:25:16: #1 tag size = 43 INFO @ Fri, 16 Oct 2020 09:25:16: #1 total tags in treatment: 1018891 INFO @ Fri, 16 Oct 2020 09:25:16: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:25:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:25:16: #1 tags after filtering in treatment: 796762 INFO @ Fri, 16 Oct 2020 09:25:16: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 16 Oct 2020 09:25:16: #1 finished! INFO @ Fri, 16 Oct 2020 09:25:16: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:25:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:25:16: #2 number of paired peaks: 78 WARNING @ Fri, 16 Oct 2020 09:25:16: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:25:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:25:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:25:27: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:25:27: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:25:31: 1000000 INFO @ Fri, 16 Oct 2020 09:25:35: 2000000 INFO @ Fri, 16 Oct 2020 09:25:40: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:25:44: 4000000 INFO @ Fri, 16 Oct 2020 09:25:45: #1 tag size is determined as 43 bps INFO @ Fri, 16 Oct 2020 09:25:45: #1 tag size = 43 INFO @ Fri, 16 Oct 2020 09:25:45: #1 total tags in treatment: 1018891 INFO @ Fri, 16 Oct 2020 09:25:45: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:25:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:25:45: #1 tags after filtering in treatment: 796762 INFO @ Fri, 16 Oct 2020 09:25:45: #1 Redundant rate of treatment: 0.22 INFO @ Fri, 16 Oct 2020 09:25:45: #1 finished! INFO @ Fri, 16 Oct 2020 09:25:45: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:25:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:25:45: #2 number of paired peaks: 78 WARNING @ Fri, 16 Oct 2020 09:25:45: Too few paired peaks (78) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:25:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8952635/SRX8952635.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。