Job ID = 10224063 SRX = SRX8952620 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 4896926 spots for SRR12458266/SRR12458266.sra Written 4896926 spots for SRR12458266/SRR12458266.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:05 4896926 reads; of these: 4896926 (100.00%) were paired; of these: 3714985 (75.86%) aligned concordantly 0 times 1039890 (21.24%) aligned concordantly exactly 1 time 142051 (2.90%) aligned concordantly >1 times ---- 3714985 pairs aligned concordantly 0 times; of these: 65063 (1.75%) aligned discordantly 1 time ---- 3649922 pairs aligned 0 times concordantly or discordantly; of these: 7299844 mates make up the pairs; of these: 6886968 (94.34%) aligned 0 times 324358 (4.44%) aligned exactly 1 time 88518 (1.21%) aligned >1 times 29.68% overall alignment rate Time searching: 00:01:05 Overall time: 00:01:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 202734 / 1238004 = 0.1638 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:18:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:18:32: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:18:32: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:18:37: 1000000 INFO @ Fri, 16 Oct 2020 09:18:42: 2000000 INFO @ Fri, 16 Oct 2020 09:18:44: #1 tag size is determined as 44 bps INFO @ Fri, 16 Oct 2020 09:18:44: #1 tag size = 44 INFO @ Fri, 16 Oct 2020 09:18:44: #1 total tags in treatment: 993068 INFO @ Fri, 16 Oct 2020 09:18:44: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:18:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:18:44: #1 tags after filtering in treatment: 868488 INFO @ Fri, 16 Oct 2020 09:18:44: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 16 Oct 2020 09:18:44: #1 finished! INFO @ Fri, 16 Oct 2020 09:18:44: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:18:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:18:44: #2 number of paired peaks: 190 WARNING @ Fri, 16 Oct 2020 09:18:44: Fewer paired peaks (190) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 190 pairs to build model! INFO @ Fri, 16 Oct 2020 09:18:44: start model_add_line... INFO @ Fri, 16 Oct 2020 09:18:44: start X-correlation... INFO @ Fri, 16 Oct 2020 09:18:44: end of X-cor INFO @ Fri, 16 Oct 2020 09:18:44: #2 finished! INFO @ Fri, 16 Oct 2020 09:18:44: #2 predicted fragment length is 245 bps INFO @ Fri, 16 Oct 2020 09:18:44: #2 alternative fragment length(s) may be 2,15,104,147,166,222,245,517,541,564 bps INFO @ Fri, 16 Oct 2020 09:18:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05_model.r INFO @ Fri, 16 Oct 2020 09:18:44: #3 Call peaks... INFO @ Fri, 16 Oct 2020 09:18:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 09:18:47: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 09:18:47: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05_peaks.xls INFO @ Fri, 16 Oct 2020 09:18:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 09:18:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.05_summits.bed INFO @ Fri, 16 Oct 2020 09:18:47: Done! pass1 - making usageList (9 chroms): 0 millis pass2 - checking and writing primary data (19 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:19:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:19:02: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:19:02: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:19:08: 1000000 INFO @ Fri, 16 Oct 2020 09:19:14: 2000000 INFO @ Fri, 16 Oct 2020 09:19:16: #1 tag size is determined as 44 bps INFO @ Fri, 16 Oct 2020 09:19:16: #1 tag size = 44 INFO @ Fri, 16 Oct 2020 09:19:16: #1 total tags in treatment: 993068 INFO @ Fri, 16 Oct 2020 09:19:16: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:19:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:19:16: #1 tags after filtering in treatment: 868488 INFO @ Fri, 16 Oct 2020 09:19:16: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 16 Oct 2020 09:19:16: #1 finished! INFO @ Fri, 16 Oct 2020 09:19:16: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:19:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:19:17: #2 number of paired peaks: 190 WARNING @ Fri, 16 Oct 2020 09:19:17: Fewer paired peaks (190) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 190 pairs to build model! INFO @ Fri, 16 Oct 2020 09:19:17: start model_add_line... INFO @ Fri, 16 Oct 2020 09:19:17: start X-correlation... INFO @ Fri, 16 Oct 2020 09:19:17: end of X-cor INFO @ Fri, 16 Oct 2020 09:19:17: #2 finished! INFO @ Fri, 16 Oct 2020 09:19:17: #2 predicted fragment length is 245 bps INFO @ Fri, 16 Oct 2020 09:19:17: #2 alternative fragment length(s) may be 2,15,104,147,166,222,245,517,541,564 bps INFO @ Fri, 16 Oct 2020 09:19:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10_model.r INFO @ Fri, 16 Oct 2020 09:19:17: #3 Call peaks... INFO @ Fri, 16 Oct 2020 09:19:17: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 09:19:19: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 09:19:20: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10_peaks.xls INFO @ Fri, 16 Oct 2020 09:19:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 09:19:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.10_summits.bed INFO @ Fri, 16 Oct 2020 09:19:20: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (18 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:19:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:19:32: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:19:32: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:19:37: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:19:42: 2000000 INFO @ Fri, 16 Oct 2020 09:19:44: #1 tag size is determined as 44 bps INFO @ Fri, 16 Oct 2020 09:19:44: #1 tag size = 44 INFO @ Fri, 16 Oct 2020 09:19:44: #1 total tags in treatment: 993068 INFO @ Fri, 16 Oct 2020 09:19:44: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:19:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:19:44: #1 tags after filtering in treatment: 868488 INFO @ Fri, 16 Oct 2020 09:19:44: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 16 Oct 2020 09:19:44: #1 finished! INFO @ Fri, 16 Oct 2020 09:19:44: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:19:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:19:44: #2 number of paired peaks: 190 WARNING @ Fri, 16 Oct 2020 09:19:44: Fewer paired peaks (190) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 190 pairs to build model! INFO @ Fri, 16 Oct 2020 09:19:44: start model_add_line... INFO @ Fri, 16 Oct 2020 09:19:44: start X-correlation... INFO @ Fri, 16 Oct 2020 09:19:44: end of X-cor INFO @ Fri, 16 Oct 2020 09:19:44: #2 finished! INFO @ Fri, 16 Oct 2020 09:19:44: #2 predicted fragment length is 245 bps INFO @ Fri, 16 Oct 2020 09:19:44: #2 alternative fragment length(s) may be 2,15,104,147,166,222,245,517,541,564 bps INFO @ Fri, 16 Oct 2020 09:19:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20_model.r INFO @ Fri, 16 Oct 2020 09:19:44: #3 Call peaks... INFO @ Fri, 16 Oct 2020 09:19:44: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:19:47: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 09:19:48: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20_peaks.xls INFO @ Fri, 16 Oct 2020 09:19:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 09:19:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX8952620/SRX8952620.20_summits.bed INFO @ Fri, 16 Oct 2020 09:19:48: Done! pass1 - making usageList (3 chroms): 0 millis pass2 - checking and writing primary data (5 records, 4 fields): 2 millis CompletedMACS2peakCalling