Job ID = 14521505 SRX = SRX8833553 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 33958016 spots for SRR12333589/SRR12333589.sra Written 33958016 spots for SRR12333589/SRR12333589.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:42 33958016 reads; of these: 33958016 (100.00%) were unpaired; of these: 5853596 (17.24%) aligned 0 times 7445043 (21.92%) aligned exactly 1 time 20659377 (60.84%) aligned >1 times 82.76% overall alignment rate Time searching: 00:03:42 Overall time: 00:03:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 23207301 / 28104420 = 0.8258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:09: 1000000 INFO @ Sat, 15 Jan 2022 21:14:14: 2000000 INFO @ Sat, 15 Jan 2022 21:14:20: 3000000 INFO @ Sat, 15 Jan 2022 21:14:26: 4000000 INFO @ Sat, 15 Jan 2022 21:14:30: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:14:30: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:14:30: #1 total tags in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:14:30: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:30: #1 tags after filtering in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:14:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:30: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:31: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:14:33: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:14:33: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:14:39: 1000000 INFO @ Sat, 15 Jan 2022 21:14:44: 2000000 INFO @ Sat, 15 Jan 2022 21:14:50: 3000000 INFO @ Sat, 15 Jan 2022 21:14:55: 4000000 INFO @ Sat, 15 Jan 2022 21:15:00: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:15:00: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:15:00: #1 total tags in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:15:00: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:00: #1 tags after filtering in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:15:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:00: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:00: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:00: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:15:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:15:03: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:15:03: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:15:10: 1000000 INFO @ Sat, 15 Jan 2022 21:15:17: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:15:24: 3000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:15:31: 4000000 INFO @ Sat, 15 Jan 2022 21:15:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:15:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:15:37: #1 total tags in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:15:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:15:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:15:37: #1 tags after filtering in treatment: 4897119 INFO @ Sat, 15 Jan 2022 21:15:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:15:37: #1 finished! INFO @ Sat, 15 Jan 2022 21:15:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:15:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:15:37: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:15:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:15:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833553/SRX8833553.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling