Job ID = 14521504 SRX = SRX8833552 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27956386 spots for SRR12333588/SRR12333588.sra Written 27956386 spots for SRR12333588/SRR12333588.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:22 27956386 reads; of these: 27956386 (100.00%) were unpaired; of these: 5306116 (18.98%) aligned 0 times 20355721 (72.81%) aligned exactly 1 time 2294549 (8.21%) aligned >1 times 81.02% overall alignment rate Time searching: 00:04:22 Overall time: 00:04:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14338223 / 22650270 = 0.6330 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:18:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:18:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:18:53: 1000000 INFO @ Sat, 15 Jan 2022 21:18:58: 2000000 INFO @ Sat, 15 Jan 2022 21:19:03: 3000000 INFO @ Sat, 15 Jan 2022 21:19:09: 4000000 INFO @ Sat, 15 Jan 2022 21:19:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:19:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:19:18: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:19:18: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:19:19: 6000000 INFO @ Sat, 15 Jan 2022 21:19:25: 1000000 INFO @ Sat, 15 Jan 2022 21:19:25: 7000000 INFO @ Sat, 15 Jan 2022 21:19:32: 8000000 INFO @ Sat, 15 Jan 2022 21:19:32: 2000000 INFO @ Sat, 15 Jan 2022 21:19:34: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:19:34: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:19:34: #1 total tags in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:19:34: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:19:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:19:34: #1 tags after filtering in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:19:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:19:34: #1 finished! INFO @ Sat, 15 Jan 2022 21:19:34: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:19:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:19:34: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:19:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:19:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:19:39: 3000000 INFO @ Sat, 15 Jan 2022 21:19:45: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:19:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:19:48: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:19:48: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:19:52: 5000000 INFO @ Sat, 15 Jan 2022 21:19:55: 1000000 INFO @ Sat, 15 Jan 2022 21:19:59: 6000000 INFO @ Sat, 15 Jan 2022 21:20:02: 2000000 INFO @ Sat, 15 Jan 2022 21:20:06: 7000000 INFO @ Sat, 15 Jan 2022 21:20:08: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:20:14: 8000000 INFO @ Sat, 15 Jan 2022 21:20:14: 4000000 INFO @ Sat, 15 Jan 2022 21:20:16: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:20:16: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:20:16: #1 total tags in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:20:16: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:20:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:20:16: #1 tags after filtering in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:20:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:20:16: #1 finished! INFO @ Sat, 15 Jan 2022 21:20:16: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:20:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:20:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:20:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:20:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:20:20: 5000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:20:25: 6000000 INFO @ Sat, 15 Jan 2022 21:20:30: 7000000 INFO @ Sat, 15 Jan 2022 21:20:36: 8000000 INFO @ Sat, 15 Jan 2022 21:20:37: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:20:37: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:20:37: #1 total tags in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:20:37: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:20:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:20:37: #1 tags after filtering in treatment: 8312047 INFO @ Sat, 15 Jan 2022 21:20:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:20:37: #1 finished! INFO @ Sat, 15 Jan 2022 21:20:37: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:20:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:20:38: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:20:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:20:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833552/SRX8833552.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling