Job ID = 14521503 SRX = SRX8833551 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 24873169 spots for SRR12333587/SRR12333587.sra Written 24873169 spots for SRR12333587/SRR12333587.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:12 24873169 reads; of these: 24873169 (100.00%) were unpaired; of these: 4378271 (17.60%) aligned 0 times 18691432 (75.15%) aligned exactly 1 time 1803466 (7.25%) aligned >1 times 82.40% overall alignment rate Time searching: 00:03:12 Overall time: 00:03:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12103045 / 20494898 = 0.5905 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:12:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:12:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:12:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:12:41: 1000000 INFO @ Sat, 15 Jan 2022 21:12:47: 2000000 INFO @ Sat, 15 Jan 2022 21:12:53: 3000000 INFO @ Sat, 15 Jan 2022 21:12:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:04: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:04: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:06: 5000000 INFO @ Sat, 15 Jan 2022 21:13:11: 1000000 INFO @ Sat, 15 Jan 2022 21:13:13: 6000000 INFO @ Sat, 15 Jan 2022 21:13:18: 2000000 INFO @ Sat, 15 Jan 2022 21:13:20: 7000000 INFO @ Sat, 15 Jan 2022 21:13:25: 3000000 INFO @ Sat, 15 Jan 2022 21:13:27: 8000000 INFO @ Sat, 15 Jan 2022 21:13:29: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:13:29: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:13:29: #1 total tags in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:13:29: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:13:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:13:30: #1 tags after filtering in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:13:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:13:30: #1 finished! INFO @ Sat, 15 Jan 2022 21:13:30: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:13:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:13:30: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:13:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:13:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:13:32: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:13:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:13:34: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:13:34: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:13:39: 5000000 INFO @ Sat, 15 Jan 2022 21:13:41: 1000000 INFO @ Sat, 15 Jan 2022 21:13:46: 6000000 INFO @ Sat, 15 Jan 2022 21:13:48: 2000000 INFO @ Sat, 15 Jan 2022 21:13:53: 7000000 INFO @ Sat, 15 Jan 2022 21:13:55: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:14:00: 8000000 INFO @ Sat, 15 Jan 2022 21:14:02: 4000000 INFO @ Sat, 15 Jan 2022 21:14:02: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:14:02: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:14:02: #1 total tags in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:14:02: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:03: #1 tags after filtering in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:14:03: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:03: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:03: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:03: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:14:09: 5000000 INFO @ Sat, 15 Jan 2022 21:14:15: 6000000 INFO @ Sat, 15 Jan 2022 21:14:22: 7000000 INFO @ Sat, 15 Jan 2022 21:14:29: 8000000 INFO @ Sat, 15 Jan 2022 21:14:31: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:14:31: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:14:31: #1 total tags in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:14:31: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:14:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:14:31: #1 tags after filtering in treatment: 8391853 INFO @ Sat, 15 Jan 2022 21:14:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:14:31: #1 finished! INFO @ Sat, 15 Jan 2022 21:14:31: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:14:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:14:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:14:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:14:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833551/SRX8833551.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling