Job ID = 14521478 SRX = SRX8833548 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 38977593 spots for SRR12333584/SRR12333584.sra Written 38977593 spots for SRR12333584/SRR12333584.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:35 38977593 reads; of these: 38977593 (100.00%) were unpaired; of these: 8056048 (20.67%) aligned 0 times 21491269 (55.14%) aligned exactly 1 time 9430276 (24.19%) aligned >1 times 79.33% overall alignment rate Time searching: 00:04:35 Overall time: 00:04:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21793062 / 30921545 = 0.7048 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:15:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:15:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:15:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:15:45: 1000000 INFO @ Sat, 15 Jan 2022 21:15:50: 2000000 INFO @ Sat, 15 Jan 2022 21:15:54: 3000000 INFO @ Sat, 15 Jan 2022 21:15:59: 4000000 INFO @ Sat, 15 Jan 2022 21:16:03: 5000000 INFO @ Sat, 15 Jan 2022 21:16:08: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:16:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:16:11: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:16:11: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:16:12: 7000000 INFO @ Sat, 15 Jan 2022 21:16:16: 1000000 INFO @ Sat, 15 Jan 2022 21:16:17: 8000000 INFO @ Sat, 15 Jan 2022 21:16:20: 2000000 INFO @ Sat, 15 Jan 2022 21:16:22: 9000000 INFO @ Sat, 15 Jan 2022 21:16:22: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:16:22: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:16:22: #1 total tags in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:16:22: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:23: #1 tags after filtering in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:16:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:23: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:25: 3000000 INFO @ Sat, 15 Jan 2022 21:16:30: 4000000 INFO @ Sat, 15 Jan 2022 21:16:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 15 Jan 2022 21:16:39: 6000000 INFO @ Sat, 15 Jan 2022 21:16:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Jan 2022 21:16:41: #1 read tag files... INFO @ Sat, 15 Jan 2022 21:16:41: #1 read treatment tags... INFO @ Sat, 15 Jan 2022 21:16:44: 7000000 INFO @ Sat, 15 Jan 2022 21:16:45: 1000000 INFO @ Sat, 15 Jan 2022 21:16:48: 8000000 INFO @ Sat, 15 Jan 2022 21:16:50: 2000000 INFO @ Sat, 15 Jan 2022 21:16:53: 9000000 INFO @ Sat, 15 Jan 2022 21:16:54: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:16:54: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:16:54: #1 total tags in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:16:54: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:16:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:16:54: #1 tags after filtering in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:16:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:16:54: #1 finished! INFO @ Sat, 15 Jan 2022 21:16:54: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:16:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:16:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:16:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:16:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 15 Jan 2022 21:16:55: 3000000 INFO @ Sat, 15 Jan 2022 21:16:59: 4000000 INFO @ Sat, 15 Jan 2022 21:17:04: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 15 Jan 2022 21:17:09: 6000000 INFO @ Sat, 15 Jan 2022 21:17:13: 7000000 BigWig に変換しました。 INFO @ Sat, 15 Jan 2022 21:17:18: 8000000 INFO @ Sat, 15 Jan 2022 21:17:23: 9000000 INFO @ Sat, 15 Jan 2022 21:17:23: #1 tag size is determined as 50 bps INFO @ Sat, 15 Jan 2022 21:17:23: #1 tag size = 50 INFO @ Sat, 15 Jan 2022 21:17:23: #1 total tags in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:17:23: #1 user defined the maximum tags... INFO @ Sat, 15 Jan 2022 21:17:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Jan 2022 21:17:23: #1 tags after filtering in treatment: 9128483 INFO @ Sat, 15 Jan 2022 21:17:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Jan 2022 21:17:23: #1 finished! INFO @ Sat, 15 Jan 2022 21:17:23: #2 Build Peak Model... INFO @ Sat, 15 Jan 2022 21:17:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Jan 2022 21:17:24: #2 number of paired peaks: 0 WARNING @ Sat, 15 Jan 2022 21:17:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Jan 2022 21:17:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX8833548/SRX8833548.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling